##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546465_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1285639
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64025049022315	31.0	31.0	34.0	30.0	34.0
2	32.012841085250216	31.0	31.0	34.0	30.0	34.0
3	32.11058236410065	33.0	31.0	34.0	30.0	34.0
4	35.78907920497122	37.0	35.0	37.0	35.0	37.0
5	35.73416332267456	37.0	35.0	37.0	35.0	37.0
6	35.794488966187245	37.0	35.0	37.0	35.0	37.0
7	36.1171044126695	37.0	35.0	37.0	35.0	37.0
8	18.00833826602958	0.0	0.0	37.0	0.0	37.0
9	27.744148240680314	19.0	17.0	39.0	17.0	39.0
10	33.70928542149079	32.0	32.0	39.0	27.0	39.0
11	35.87794707534541	37.0	35.0	39.0	32.0	39.0
12	36.561638998194674	37.0	35.0	39.0	33.0	39.0
13	36.701280841667064	39.0	35.0	39.0	33.0	39.0
14	37.87325057811719	40.0	36.0	41.0	33.0	41.0
15	38.14848024989908	40.0	37.0	41.0	33.0	41.0
16	38.240996111661204	40.0	37.0	41.0	34.0	41.0
17	38.21081189976346	40.0	37.0	41.0	34.0	41.0
18	38.17527470775233	40.0	36.0	41.0	34.0	41.0
19	38.181079603216766	40.0	37.0	41.0	34.0	41.0
20	38.04609692145307	40.0	36.0	41.0	34.0	41.0
21	37.9390062062523	40.0	36.0	41.0	33.0	41.0
22	37.93415414435934	40.0	36.0	41.0	33.0	41.0
23	37.81256013546571	39.0	36.0	41.0	33.0	41.0
24	37.68337379310989	39.0	35.0	41.0	33.0	41.0
25	37.577885393955846	39.0	35.0	41.0	33.0	41.0
26	37.526802624998155	39.0	35.0	41.0	33.0	41.0
27	37.356292085103206	39.0	35.0	41.0	33.0	41.0
28	37.3936890526812	39.0	35.0	41.0	33.0	41.0
29	37.30037358854235	39.0	35.0	41.0	33.0	41.0
30	37.23779847997766	39.0	35.0	41.0	33.0	41.0
31	37.191599663669194	39.0	35.0	41.0	32.0	41.0
32	36.92610833989946	39.0	35.0	41.0	31.0	41.0
33	36.79675009858911	39.0	35.0	41.0	31.0	41.0
34	36.671122297939	39.0	35.0	41.0	31.0	41.0
35	36.48323518499361	39.0	35.0	41.0	30.0	41.0
36	36.362335772328	39.0	35.0	41.0	30.0	41.0
37	36.26566400054759	39.0	35.0	41.0	30.0	41.0
38	36.2207501483698	39.0	35.0	41.0	30.0	41.0
39	36.174623669630435	39.0	35.0	41.0	30.0	41.0
40	36.10972520279799	39.0	35.0	41.0	29.0	41.0
41	36.138935579894515	39.0	35.0	41.0	30.0	41.0
42	35.987144136106636	39.0	35.0	41.0	29.0	41.0
43	36.00711397211815	39.0	35.0	41.0	29.0	41.0
44	35.79696400000311	39.0	35.0	40.0	28.0	41.0
45	35.72780306135704	39.0	35.0	40.0	28.0	41.0
46	35.72873800499207	39.0	35.0	40.0	28.0	41.0
47	35.61932470934687	38.0	35.0	40.0	27.0	41.0
48	35.63361954638899	38.0	35.0	40.0	28.0	41.0
49	35.60872453309211	38.0	35.0	40.0	28.0	41.0
50	34.98433386043827	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	4.0
14	0.0
15	18.0
16	26.0
17	100.0
18	227.0
19	554.0
20	1203.0
21	2191.0
22	3623.0
23	5755.0
24	8513.0
25	12700.0
26	17135.0
27	19230.0
28	19587.0
29	20932.0
30	25344.0
31	32751.0
32	43723.0
33	60048.0
34	92381.0
35	149348.0
36	128554.0
37	202154.0
38	236153.0
39	203380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.1244143962652	23.536700426791658	29.604655739286066	14.73422943765707
2	32.21713093644483	25.02350970995746	30.37656760568091	12.382791747916794
3	27.440984599876018	25.272413173526935	34.61819375423428	12.668408472362772
4	24.131346357725615	26.96954588340895	34.7566463058448	14.142461453020639
5	22.77381131095121	31.27542023849619	32.180962151894896	13.769806298657711
6	19.335832220397794	40.774976490290044	28.86214559452537	11.027045694786795
7	86.96072536691871	2.9510616899456226	8.625982876997353	1.4622300661383172
8	44.0552130108063	51.358740672926075	3.748252814359241	0.8377935019083895
9	84.04443237954044	4.2938958759029555	8.856140798466756	2.805530946089843
10	43.74455037533864	33.62009086532067	13.510946696545453	9.124412062795233
11	35.27133199910706	22.264648163286896	28.83523290752692	13.628786930079128
12	32.99184296680483	22.797923833984502	28.24315379356102	15.967079405649642
13	21.81553297620872	34.40265891124958	28.722681872594098	15.059126239947606
14	16.030238659530397	36.61929981900051	30.522798390527978	16.82766313094111
15	14.840791232997756	24.7002463366466	44.35381938475731	16.105143045598336
16	17.204518531251775	22.397500386967103	41.767401268940965	18.63057981284015
17	18.009876800563767	21.96767521831556	30.93310019375579	29.089347787364883
18	20.751003975455006	23.855452424825323	35.040940730640564	20.35260286907911
19	25.12190436039977	25.5353174569222	28.650499868158946	20.692278314519083
20	27.37766978133053	24.607996490461165	28.545727066462668	19.46860666174564
21	21.870213955861637	27.610005608106164	30.939556127342122	19.580224308690074
22	21.9029603177875	23.68736480458356	30.578334975836917	23.831339901792028
23	18.599311315229237	28.802953239595254	30.06644944653981	22.531285998635695
24	18.61743459867039	24.842976916537225	38.339067187600875	18.200521297191514
25	17.081933575443806	27.4892096459426	35.17947106458345	20.249385714030144
26	18.143662412232363	32.220786706066015	29.783632886058996	19.851917995642633
27	18.41520053452019	30.150298800829784	31.754092711873238	19.68040795277679
28	15.989869629032722	27.332478246226195	38.34723433249925	18.330417792241835
29	16.5486579047462	24.509912969348317	37.6273588464569	21.314070279448586
30	19.465962062445215	28.357493822138252	32.85883517845989	19.31770893695664
31	25.822023134021293	25.6666140339551	28.125235777694986	20.386127054328625
32	26.17686613427253	27.00781479093276	28.089689251803968	18.725629822990747
33	24.703824323935414	27.478942378070357	28.344037478639027	19.473195819355198
34	19.77009098199417	27.64881899195653	30.245037681650917	22.33605234439839
35	20.291310391175127	26.322396878128306	32.02415296984613	21.36213976085044
36	25.831590360902247	26.5057298355137	28.478756478296006	19.183923325288045
37	20.742214571897712	29.82874663883096	29.84305858798621	19.585980201285118
38	19.89283150246687	30.72114333805991	27.74643581907518	21.639589340398043
39	20.365281389254683	30.188256579024124	28.405329956543014	21.04113207517818
40	22.07602600730065	27.38350345625794	27.681643136214756	22.85882740022666
41	18.90608483407862	25.297381302216255	28.440254223775103	27.356279639930026
42	21.571374234913534	27.219382734966814	26.250914914684447	24.958328115435204
43	21.52804947578597	27.184536250067087	27.886988493659576	23.400425780487367
44	20.40673937240547	29.46223628872491	29.105448730164536	21.02557560870509
45	18.681138328877704	32.18990712011692	26.341920243552043	22.787034307453336
46	21.8187220518357	30.330442682588192	27.96928220130223	19.881553064273874
47	20.937681573132117	27.12083251985977	28.993675518555364	22.947810388452748
48	21.803554497024436	24.879767959746086	30.908520976728305	22.408156566501173
49	20.773094157846796	24.186882943034554	31.940070268559058	23.09995263055959
50	19.04212613338581	29.950009294988718	29.4714923862764	21.536372185349077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	306.0
1	657.5
2	1009.0
3	8913.0
4	16817.0
5	11810.5
6	6804.0
7	6968.5
8	7133.0
9	7473.0
10	7813.0
11	7767.5
12	7722.0
13	7511.0
14	7300.0
15	6820.0
16	6340.0
17	6156.0
18	5972.0
19	5998.0
20	6024.0
21	6365.0
22	6706.0
23	7354.5
24	8003.0
25	9540.5
26	11078.0
27	13694.0
28	16310.0
29	19751.5
30	23193.0
31	27065.0
32	30937.0
33	34548.0
34	38159.0
35	42616.5
36	47074.0
37	52376.0
38	57678.0
39	70593.0
40	83508.0
41	96715.0
42	109922.0
43	116059.5
44	122197.0
45	124784.0
46	127371.0
47	123758.0
48	120145.0
49	113352.5
50	106560.0
51	95071.0
52	83582.0
53	74251.5
54	64921.0
55	57101.5
56	49282.0
57	42576.5
58	35871.0
59	31171.5
60	26472.0
61	22352.5
62	18233.0
63	14732.5
64	11232.0
65	8664.5
66	6097.0
67	4749.5
68	3402.0
69	2686.5
70	1971.0
71	1614.0
72	1257.0
73	955.5
74	654.0
75	501.0
76	348.0
77	246.0
78	144.0
79	98.5
80	53.0
81	39.0
82	25.0
83	16.0
84	7.0
85	4.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1285639.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.211073663801525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.49723947178988	22.627166815762937
2	6.924700730040227	4.322546891701315
3	3.2450360077560574	3.038431736392882
4	2.1970166365997637	2.7428499234205073
5	1.6062481393159456	2.5066364499267055
6	1.3793077933153122	2.582980628533184
7	1.2062886999486473	2.635469583173602
8	1.013891329462632	2.5315709576758287
9	0.9257450233992305	2.6004146507289945
>10	8.906287328766204	50.80816596792881
>50	0.08270457795233757	1.6694074302190214
>100	0.013780393604607066	0.771522736597798
>500	0.0010022103138147454	0.22279222685921457
>1k	2.5055257845368634E-4	0.12472923897523597
>5k	5.011051569073727E-4	0.8153147621039563
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5377	0.4182356011290883	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5049	0.39272299611321687	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1595	0.12406282012291164	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04604714076035341	0.0
2	0.0	0.0	0.0	0.13082988303870682	0.0
3	0.0	0.0	0.0	0.2016118054912771	0.0
4	0.0	0.0	0.0	0.2900503173908072	0.0
5	0.0	0.0	0.0	0.5054295957107711	0.0
6	0.0	0.0	0.0	0.7569776585806747	0.0
7	0.0	0.0	0.0	0.9331546413884457	0.0
8	0.0	0.0	0.0	1.295775874876229	0.0
9	0.0	0.0	0.0	1.6422961655643613	0.0
10	0.0	0.0	0.0	2.1793831705478754	0.0
11	0.0	0.0	0.0	2.609286121531783	0.0
12	0.0	0.0	0.0	3.0472784350816986	0.0
13	0.0	0.0	0.0	3.2383118433712728	0.0
14	0.0	0.0	0.0	3.3246502322969356	0.0
15	0.0	0.0	0.0	3.4359567499119117	0.0
16	0.0	0.0	0.0	3.637646337735554	0.0
17	0.0	0.0	0.0	3.8900500062614776	0.0
18	0.0	0.0	0.0	4.227703111059948	0.0
19	0.0	0.0	0.0	4.423636806288546	0.0
20	0.0	0.0	0.0	4.610469968630385	0.0
21	0.0	0.0	0.0	4.829193887242064	0.0
22	0.0	0.0	0.0	5.066274436291992	0.0
23	0.0	0.0	0.0	5.340223810883148	0.0
24	0.0	0.0	0.0	5.544946909669044	0.0
25	0.0	0.0	0.0	5.7194126811647745	0.0
26	0.0	0.0	0.0	5.8922450236808315	0.0
27	0.0	0.0	0.0	6.076978063048803	0.0
28	0.0	0.0	0.0	6.26762255967655	0.0
29	0.0	0.0	0.0	6.466667548199767	0.0
30	0.0	0.0	0.0	6.726693885297506	0.0
31	0.0	0.0	0.0	6.953118254813365	0.0
32	0.0	0.0	0.0	7.15340776065443	0.0
33	0.0	0.0	0.0	7.358441988769787	0.0
34	0.0	0.0	0.0	7.566431945515032	0.0
35	0.0	0.0	0.0	7.813313068443008	0.0
36	0.0	0.0	0.0	8.032425898716514	0.0
37	0.0	0.0	0.0	8.257450186249795	0.0
38	0.0	0.0	0.0	8.487141413725004	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGA	25	4.4431363E-5	44.0	2
TAATGCG	50	2.7284841E-11	44.0	1
CGGCGAA	35	1.4462603E-7	43.999996	31
AAACGCG	35	1.4462603E-7	43.999996	1
TATAGCG	70	0.0	43.999996	1
CGACGGT	235	0.0	43.06383	28
CGTTTTT	7030	0.0	42.779514	1
TAAGCGA	130	0.0	42.30769	2
AATGCGG	100	0.0	41.8	2
ATTGCGA	90	0.0	41.555553	2
AACGCGA	65	0.0	40.615383	2
GCGTATG	125	0.0	40.48	1
GTAAGCG	60	3.6379788E-12	40.333332	1
GACCGAT	415	0.0	40.289154	9
GAGCGAT	765	0.0	40.261436	7
CGGGTAT	55	7.8216544E-11	40.0	6
TTTGCGA	160	0.0	39.875	2
CTCACGA	250	0.0	39.6	24
TAGCGCG	50	1.3478711E-9	39.6	1
GTTTTTA	4145	0.0	39.276234	2
>>END_MODULE