FastQCFastQC Report
Thu 26 May 2016
SRR1546464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546464_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1769319
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100750.5694281246061338No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93750.5298648802166257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG700.044.01
CGTTAGA1150.044.02
TCTACGA302.5284935E-644.02
TCGTATA254.443818E-544.012
TTAGTCG302.5284935E-644.01
CGATCGT207.857635E-444.010
TCGGTAC454.802132E-1044.036
CGTTTTT132500.043.219621
CGACGGT4450.043.0112428
CGGTCTA4450.042.51685331
GAGCGAT10400.041.038467
GTTTTTA74350.040.5971762
CGATTAC557.8216544E-1140.010
TGCGCGA1100.040.02
ACGGTCT4750.039.83157730
CACGACG4850.039.46391726
CCGGATA452.3521352E-839.1111120
GACGGTC5000.038.7229
ACGAGAT3950.038.430385
TCACGAC5100.038.3921625
Produced by FastQC (version 0.11.5)