##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546464_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1769319
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.535655243627634	31.0	31.0	34.0	30.0	34.0
2	31.910645282167884	31.0	31.0	34.0	30.0	34.0
3	31.985051310702026	33.0	31.0	34.0	30.0	34.0
4	35.68548011975229	37.0	35.0	37.0	33.0	37.0
5	35.63472160757896	37.0	35.0	37.0	33.0	37.0
6	35.68275082107862	37.0	35.0	37.0	33.0	37.0
7	36.05748482890875	37.0	35.0	37.0	35.0	37.0
8	18.242601814596462	26.0	0.0	37.0	0.0	37.0
9	27.829790444798252	25.0	17.0	39.0	17.0	39.0
10	33.61675650349089	32.0	32.0	39.0	27.0	39.0
11	35.80222051535082	37.0	35.0	39.0	32.0	39.0
12	36.53246701131904	37.0	35.0	39.0	33.0	39.0
13	36.70086117879252	39.0	35.0	39.0	33.0	39.0
14	37.86885914863289	40.0	36.0	41.0	33.0	41.0
15	38.121240432053234	40.0	37.0	41.0	33.0	41.0
16	38.1919105599386	40.0	37.0	41.0	33.0	41.0
17	38.147912841042235	40.0	37.0	41.0	33.0	41.0
18	38.095850437371666	40.0	37.0	41.0	33.0	41.0
19	38.07909144704827	40.0	37.0	41.0	34.0	41.0
20	37.95061941910984	40.0	36.0	41.0	33.0	41.0
21	37.82442962518348	40.0	36.0	41.0	33.0	41.0
22	37.83220380270601	40.0	36.0	41.0	33.0	41.0
23	37.66679835575156	39.0	36.0	41.0	33.0	41.0
24	37.513567649474176	39.0	35.0	41.0	33.0	41.0
25	37.37959350461957	39.0	35.0	41.0	33.0	41.0
26	37.33760729410581	39.0	35.0	41.0	33.0	41.0
27	37.17449368937992	39.0	35.0	41.0	32.0	41.0
28	37.16360192819949	39.0	35.0	41.0	32.0	41.0
29	37.087123916037754	39.0	35.0	41.0	32.0	41.0
30	36.96335934899246	39.0	35.0	41.0	32.0	41.0
31	36.892208245093165	39.0	35.0	41.0	31.0	41.0
32	36.58805902157836	39.0	35.0	41.0	30.0	41.0
33	36.38495149828832	39.0	35.0	41.0	30.0	41.0
34	36.199853163844395	39.0	35.0	41.0	30.0	41.0
35	35.984595203013136	39.0	35.0	41.0	28.0	41.0
36	35.832809120345175	39.0	35.0	41.0	27.0	41.0
37	35.75585182773711	39.0	35.0	41.0	27.0	41.0
38	35.68552363932112	39.0	35.0	41.0	26.0	41.0
39	35.593583180873544	39.0	35.0	41.0	25.0	41.0
40	35.53966243509508	39.0	35.0	41.0	25.0	41.0
41	35.53721290507817	39.0	35.0	40.0	25.0	41.0
42	35.3819932979864	39.0	35.0	40.0	24.0	41.0
43	35.39541428086173	39.0	35.0	40.0	25.0	41.0
44	35.16643861282222	38.0	35.0	40.0	24.0	41.0
45	35.08731664555685	38.0	34.0	40.0	23.0	41.0
46	35.097923551377676	38.0	34.0	40.0	24.0	41.0
47	34.99005436554968	38.0	34.0	40.0	23.0	41.0
48	34.98716568352005	38.0	34.0	40.0	23.0	41.0
49	34.96189381338244	38.0	34.0	40.0	24.0	41.0
50	34.35626418978149	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	5.0
14	14.0
15	19.0
16	70.0
17	209.0
18	544.0
19	1140.0
20	2398.0
21	3849.0
22	6535.0
23	9923.0
24	14857.0
25	22335.0
26	30007.0
27	33079.0
28	32864.0
29	34112.0
30	39217.0
31	49453.0
32	63486.0
33	83496.0
34	123370.0
35	190584.0
36	176834.0
37	272268.0
38	307726.0
39	270923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.388404239145117	23.052993835481335	29.049820863281294	16.50878106209225
2	34.388711136883735	24.04676601562522	29.394247165152244	12.170275682338797
3	27.38833415568363	24.441381118950286	35.82926538402628	12.341019341339804
4	24.11978846098414	26.649518826169842	35.521689418358136	13.709003294487879
5	21.859257714408763	30.925514279787876	33.603324216831446	13.611903788971915
6	18.996913501748413	39.97470213115894	30.67332685626504	10.355057510827612
7	84.56784785558737	2.8560706124785864	10.821903794623807	1.7541777373102307
8	43.20588881937062	50.54419242657768	5.022779950930273	1.227138803121427
9	80.52058447346126	4.60860930109268	11.942504432496346	2.928301792949717
10	38.4200927023335	34.34016138412576	16.606558794654894	10.633187118885854
11	32.28575514081972	21.612326550497677	31.816422024519035	14.285496284163568
12	30.096381715224897	21.859088157647093	30.947556658804885	17.096973468323124
13	21.528848104835816	30.184833825895723	32.054592755743876	16.23172531352458
14	16.582199139895067	32.843879481314566	33.15411183624886	17.419809542541508
15	15.44272118255668	24.116510363591868	43.664087708321674	16.776680745529777
16	17.83641050596303	22.148069398452172	41.70604622456436	18.309473871020433
17	18.549622764464747	22.832457007470104	32.47339795706709	26.14452227099805
18	20.54728401153212	24.113967012166828	35.57249992793838	19.766249048362674
19	23.58398909410909	25.74888982710297	30.77506091326663	19.89206016552131
20	25.047942174362	24.66966103907775	30.463132990715636	19.819263795844616
21	21.819129280813694	27.156267467878887	31.950710979761137	19.073892271546285
22	21.475663800592205	23.11827318872402	31.777480488255648	23.628582522428122
23	18.598115998302173	28.17829910830099	31.306677880020505	21.91690701337633
24	18.41895102013826	25.018778411354877	37.2616809066087	19.300589661898165
25	18.153029498920205	26.74452713162522	34.85476615579214	20.24767721366243
26	18.3464937639849	30.03873241625733	31.284126830718485	20.330646989039288
27	18.935025283739108	28.284950311390993	32.72338114268823	20.056643262181666
28	16.992922135578716	26.713950395604186	37.62995819295447	18.663169275862636
29	18.298678757194153	23.92881102842393	36.302046154480905	21.470464059901012
30	20.0872199982027	26.437629393003746	33.38849579979642	20.086654808997135
31	24.268150627444797	24.728553754297558	29.24475462028046	21.75854099797719
32	25.65936385694157	25.211564449372894	29.72669145586522	19.402380237820314
33	23.1373765838721	25.445552780476554	30.018837756221462	21.398232879429884
34	19.48421963478604	25.78958344990361	32.00824724088759	22.71794967442276
35	19.86939607837818	25.614996504304766	32.29474164918819	22.220865768128867
36	24.950503555322697	24.762747701234204	29.95135416507707	20.335394578366024
37	20.39089615835245	28.696747166565217	30.73741931217604	20.17493736290629
38	20.502294950769194	29.303025627374147	28.611177520842766	21.583501901013893
39	20.522924356772297	28.63757185674262	29.678706892312807	21.160796894172275
40	22.61372878491668	25.47980324633376	29.242663420219873	22.66380454852969
41	19.36841236656589	24.8983365916491	29.766198181334175	25.967052860450828
42	21.75198480319264	26.23342653303333	27.791483616012712	24.223105047761315
43	21.255918237468766	26.456732788151825	29.19756132161583	23.089787652763576
44	21.112134103573183	28.447611764752427	29.619757658172436	20.82049647350195
45	19.49134101877615	29.82503437763343	27.822625541239315	22.86099906235111
46	21.641094681060906	28.236231001871342	28.860708555099446	21.261965761968305
47	21.027864393023528	25.663772332744973	29.892065817413364	23.416297456818132
48	21.594579609442953	24.35569843538672	30.9542824103511	23.09543954481922
49	20.843612712009534	23.79881751114412	31.70287551312115	23.654694263725197
50	19.724142452548126	27.4078331832756	30.563171480100536	22.30485288407574
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	389.0
1	897.5
2	1406.0
3	16215.0
4	31024.0
5	21934.0
6	12844.0
7	12932.0
8	13020.0
9	13495.0
10	13970.0
11	13949.5
12	13929.0
13	13498.0
14	13067.0
15	12679.5
16	12292.0
17	11618.0
18	10944.0
19	10631.0
20	10318.0
21	10323.5
22	10329.0
23	10905.5
24	11482.0
25	13417.0
26	15352.0
27	18086.0
28	20820.0
29	25035.5
30	29251.0
31	34048.0
32	38845.0
33	43324.0
34	47803.0
35	53758.5
36	59714.0
37	67825.5
38	75937.0
39	89961.5
40	103986.0
41	119035.5
42	134085.0
43	142898.5
44	151712.0
45	158175.5
46	164639.0
47	165821.5
48	167004.0
49	159159.0
50	151314.0
51	134375.5
52	117437.0
53	103675.0
54	89913.0
55	80123.0
56	70333.0
57	62369.0
58	54405.0
59	48953.5
60	43502.0
61	37670.5
62	31839.0
63	25700.0
64	19561.0
65	15335.0
66	11109.0
67	8755.0
68	6401.0
69	5250.5
70	4100.0
71	3385.5
72	2671.0
73	1990.5
74	1310.0
75	990.5
76	671.0
77	486.5
78	302.0
79	242.0
80	182.0
81	128.0
82	74.0
83	50.0
84	26.0
85	15.0
86	4.0
87	2.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1769319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.88093705948357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.33124995202957	25.18412401177455
2	7.1458691597204655	4.8421748647158775
3	3.2864092697447314	3.3403987685977388
4	2.168879250824483	2.9393464554721676
5	1.59840452722452	2.7077721591243784
6	1.3007071288420353	2.644150581907116
7	1.0511411085846623	2.4929552018414913
8	0.9078608687165644	2.4607341561403198
9	0.8054614598759724	2.4560810123307815
>10	7.282034086531652	46.04987403075761
>50	0.09945559456610682	2.1465320023269445
>100	0.020257408016128665	1.174816829235348
>500	0.0017462964023921624	0.3331054723361477
>1k	1.7462964023921623E-4	0.0771526706796335
>5k	1.7462964023921623E-4	0.5546827359061073
>10k+	1.7462964023921623E-4	0.5960990468537635
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10075	0.5694281246061338	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9375	0.5298648802166257	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033459200969412524	0.0
2	0.0	0.0	0.0	0.11128575457563052	0.0
3	0.0	0.0	0.0	0.19459464347582317	0.0
4	0.0	0.0	0.0	0.30876286299983213	0.0
5	0.0	1.1303784111288016E-4	0.0	0.5504377672991699	0.0
6	0.0	1.1303784111288016E-4	0.0	0.9551697574038372	0.0
7	0.0	1.1303784111288016E-4	0.0	1.2403077116110774	0.0
8	1.1303784111288016E-4	1.1303784111288016E-4	0.0	1.9215302610778497	0.0
9	1.1303784111288016E-4	1.1303784111288016E-4	0.0	2.6061439457780082	0.0
10	1.1303784111288016E-4	1.1303784111288016E-4	0.0	3.4739354520015895	0.0
11	1.1303784111288016E-4	1.1303784111288016E-4	0.0	3.960167725548643	0.0
12	1.1303784111288016E-4	1.1303784111288016E-4	0.0	4.432270268956588	0.0
13	1.1303784111288016E-4	1.1303784111288016E-4	0.0	4.676432005760408	0.0
14	1.1303784111288016E-4	1.1303784111288016E-4	0.0	4.80738634468968	0.0
15	1.6955676166932024E-4	1.1303784111288016E-4	0.0	4.927658607633784	0.0
16	1.6955676166932024E-4	1.1303784111288016E-4	0.0	5.125022678216873	0.0
17	1.6955676166932024E-4	1.1303784111288016E-4	0.0	5.363419485123938	0.0
18	1.6955676166932024E-4	1.1303784111288016E-4	0.0	5.718471344059494	0.0
19	1.6955676166932024E-4	1.1303784111288016E-4	0.0	5.91413984702589	0.0
20	1.6955676166932024E-4	1.1303784111288016E-4	0.0	6.1261423180330965	0.0
21	1.6955676166932024E-4	1.1303784111288016E-4	0.0	6.352387557020526	0.0
22	1.6955676166932024E-4	1.1303784111288016E-4	0.0	6.598470937123266	0.0
23	1.6955676166932024E-4	1.1303784111288016E-4	0.0	6.871061690966976	0.0
24	1.6955676166932024E-4	1.1303784111288016E-4	0.0	7.086059664763675	0.0
25	1.6955676166932024E-4	1.1303784111288016E-4	0.0	7.272119951235475	0.0
26	1.6955676166932024E-4	1.1303784111288016E-4	0.0	7.445633037343747	0.0
27	1.6955676166932024E-4	1.1303784111288016E-4	0.0	7.639888567296231	0.0
28	1.6955676166932024E-4	1.1303784111288016E-4	0.0	7.836800486514868	0.0
29	1.6955676166932024E-4	1.1303784111288016E-4	0.0	8.041964168134745	0.0
30	1.6955676166932024E-4	1.1303784111288016E-4	0.0	8.31958510590798	0.0
31	1.6955676166932024E-4	1.1303784111288016E-4	0.0	8.544021739437603	0.0
32	1.6955676166932024E-4	1.1303784111288016E-4	0.0	8.762071734944348	0.0
33	1.6955676166932024E-4	1.1303784111288016E-4	0.0	8.97814356823162	0.0
34	1.6955676166932024E-4	1.1303784111288016E-4	0.0	9.209362472228015	0.0
35	1.6955676166932024E-4	1.1303784111288016E-4	0.0	9.463528057970326	0.0
36	1.6955676166932024E-4	1.1303784111288016E-4	0.0	9.689151588831635	0.0
37	1.6955676166932024E-4	1.1303784111288016E-4	0.0	9.923309476696966	0.0
38	1.6955676166932024E-4	1.1303784111288016E-4	0.0	10.166227797248546	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	70	0.0	44.0	1
CGTTAGA	115	0.0	44.0	2
TCTACGA	30	2.5284935E-6	44.0	2
TCGTATA	25	4.443818E-5	44.0	12
TTAGTCG	30	2.5284935E-6	44.0	1
CGATCGT	20	7.857635E-4	44.0	10
TCGGTAC	45	4.802132E-10	44.0	36
CGTTTTT	13250	0.0	43.21962	1
CGACGGT	445	0.0	43.01124	28
CGGTCTA	445	0.0	42.516853	31
GAGCGAT	1040	0.0	41.03846	7
GTTTTTA	7435	0.0	40.597176	2
CGATTAC	55	7.8216544E-11	40.0	10
TGCGCGA	110	0.0	40.0	2
ACGGTCT	475	0.0	39.831577	30
CACGACG	485	0.0	39.463917	26
CCGGATA	45	2.3521352E-8	39.11111	20
GACGGTC	500	0.0	38.72	29
ACGAGAT	395	0.0	38.43038	5
TCACGAC	510	0.0	38.39216	25
>>END_MODULE