FastQCFastQC Report
Thu 26 May 2016
SRR1546463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546463_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1581668
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26710.16887235500749842No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21600.13656468993492946No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20970.13258155314516068No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCG302.5283225E-644.00000436
TAATCGG302.5283225E-644.00000417
ACGTTAG254.4436027E-544.01
CGTACGT207.8573823E-444.033
CACGTTA207.8573823E-444.020
TAAGACG254.4436027E-544.01
TAGTCCG207.8573823E-444.02
GTACGTA207.8573823E-444.011
CGTTTTT31800.041.7861631
TATGCGA950.041.684212
CGTAAGA850.041.4117622
AGCGATC800.041.258
ATTGCGA1400.040.8571432
CGGTCTA3800.040.52631431
GAGCGAT9150.040.1530047
AGGGGAA140700.039.653168
TCACGAC3950.039.54430425
AGACTTA11050.039.420828
CGACGGT3800.039.3684228
CACGACG3750.039.30666726
Produced by FastQC (version 0.11.5)