##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546463_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1581668
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.526656668782575	31.0	31.0	33.0	30.0	34.0
2	31.901006405895547	31.0	31.0	34.0	30.0	34.0
3	32.016904938330924	33.0	31.0	34.0	30.0	34.0
4	35.6846531636222	37.0	35.0	37.0	33.0	37.0
5	35.63633961109411	37.0	35.0	37.0	33.0	37.0
6	35.68744515283865	37.0	35.0	37.0	33.0	37.0
7	36.034499654794814	37.0	35.0	37.0	35.0	37.0
8	18.37579315001631	29.0	0.0	37.0	0.0	37.0
9	27.90850987691475	28.0	17.0	39.0	17.0	39.0
10	33.677695319118804	32.0	32.0	39.0	27.0	39.0
11	35.83039929997952	37.0	35.0	39.0	32.0	39.0
12	36.33346631530764	37.0	35.0	39.0	32.0	39.0
13	36.36594468624263	38.0	35.0	39.0	32.0	39.0
14	37.47467610143216	39.0	36.0	41.0	33.0	41.0
15	37.79559490360809	40.0	36.0	41.0	33.0	41.0
16	37.92196718906875	40.0	36.0	41.0	33.0	41.0
17	37.89019819582871	40.0	36.0	41.0	33.0	41.0
18	37.89426099535427	40.0	36.0	41.0	33.0	41.0
19	37.903189544202704	40.0	36.0	41.0	33.0	41.0
20	37.79056160964248	40.0	36.0	41.0	33.0	41.0
21	37.64015014528966	39.0	35.0	41.0	33.0	41.0
22	37.6362669030416	39.0	35.0	41.0	33.0	41.0
23	37.54090049239158	39.0	35.0	41.0	33.0	41.0
24	37.456416896592714	39.0	35.0	41.0	33.0	41.0
25	37.334773795764974	39.0	35.0	41.0	32.0	41.0
26	37.27488385678916	39.0	35.0	41.0	32.0	41.0
27	37.12457987390527	39.0	35.0	41.0	32.0	41.0
28	37.16320618486307	39.0	35.0	41.0	32.0	41.0
29	37.1024526006722	39.0	35.0	41.0	32.0	41.0
30	37.03418479731524	39.0	35.0	41.0	32.0	41.0
31	37.09735102436162	39.0	35.0	41.0	32.0	41.0
32	36.91219775578693	39.0	35.0	41.0	31.0	41.0
33	36.897797135682076	39.0	35.0	41.0	31.0	41.0
34	36.85777293338425	39.0	35.0	41.0	31.0	41.0
35	36.74531696917431	39.0	35.0	41.0	31.0	41.0
36	36.62611875564278	39.0	35.0	41.0	31.0	41.0
37	36.539385635923594	39.0	35.0	41.0	31.0	41.0
38	36.469590331219955	39.0	35.0	41.0	30.0	41.0
39	36.432944840510146	39.0	35.0	41.0	30.0	41.0
40	36.31966000450158	39.0	35.0	40.0	30.0	41.0
41	36.34532341806245	39.0	35.0	40.0	30.0	41.0
42	36.20915577731863	38.0	35.0	40.0	30.0	41.0
43	36.22086746396842	38.0	35.0	40.0	30.0	41.0
44	35.977243644051725	38.0	35.0	40.0	30.0	41.0
45	35.87997291466983	38.0	35.0	40.0	30.0	41.0
46	35.89622537725995	38.0	35.0	40.0	30.0	41.0
47	35.763937817544516	38.0	35.0	40.0	29.0	41.0
48	35.79948257156369	38.0	35.0	40.0	29.0	41.0
49	35.7838832169583	38.0	35.0	40.0	29.0	41.0
50	35.13879018858572	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	10.0
15	23.0
16	45.0
17	136.0
18	357.0
19	700.0
20	1359.0
21	2525.0
22	4028.0
23	5961.0
24	8280.0
25	11820.0
26	15665.0
27	19090.0
28	22276.0
29	26924.0
30	33877.0
31	44886.0
32	59445.0
33	80466.0
34	127935.0
35	206126.0
36	163220.0
37	250458.0
38	278927.0
39	217123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.72037115247954	23.30324695195199	29.07310510170276	12.903276793865718
2	30.758540983316347	25.239051431779615	31.230953651461625	12.771453933442418
3	30.51658122943627	25.29589016152568	30.98153341914991	13.205995189888142
4	26.624550790684264	27.51203160208084	30.26823581181386	15.595181795421038
5	22.611635311582457	32.46844470520994	29.452577911420097	15.467342071787504
6	20.82504040038744	41.6091113937944	26.785456872112224	10.780391333705936
7	88.51528892283336	3.701851463138914	5.875948682024293	1.9069109320034292
8	45.54767498615386	50.583434703110896	2.5358039740324774	1.333086336702772
9	85.09706208888338	4.635233184208063	6.666506498203163	3.601198228705392
10	49.444889825171906	28.38446500782718	11.311349790221463	10.85929537677945
11	44.4542091007721	20.311784774048665	20.271574059790044	14.962432065389198
12	39.140767847614036	21.199392034232215	23.174774984383575	16.48506513377017
13	23.503794728097173	36.173520612416766	24.27759808000162	16.045086579484444
14	16.944832923217767	39.678238416658864	26.251842991070184	17.125085669053178
15	15.170187422392056	23.971908137485237	44.31928824506787	16.538616195054843
16	17.472566935665387	19.029341176530092	42.76308302374455	20.735008864059967
17	17.853430682039466	20.10687451475278	27.227268933809118	34.81242586939863
18	22.13764203359997	23.826492032461932	31.131185558536938	22.90468037540116
19	28.1070995935936	26.04541534633058	24.364215499080718	21.4832695609951
20	30.327793190479923	23.630433188254425	23.445944408055293	22.595829213210358
21	24.054479195381077	28.082758202100568	26.1237503698627	21.739012232655654
22	23.28978015613896	23.894142133494515	25.40444644514525	27.41163126522127
23	21.22803268448246	30.342461249769233	23.893130543198698	24.536375522549612
24	21.216272947293614	24.708661994805485	34.22070877074076	19.854356287160137
25	19.45762321802047	25.24341391493031	31.947349254078606	23.351613612970613
26	18.34038496068707	32.18374526133171	26.070136084184544	23.405733693796677
27	20.44683207853987	29.189121863753957	28.34615102537321	22.017895032332955
28	17.23655027477321	27.08975587797186	36.6499796417453	19.023714205509627
29	18.01142844136696	24.377619070500256	34.45716800238735	23.15378448574543
30	20.322406472154714	26.84001952369271	30.533082796136735	22.304491208015843
31	29.325560104901914	23.961412888166166	23.73652372052795	22.976503286403975
32	28.280777002506213	26.320441458005096	25.008977863875355	20.38980367561334
33	26.403202189081398	25.41677520187549	25.178672135998198	23.001350473044912
34	20.23275428218817	25.88526795762448	28.787267618741737	25.094710141445614
35	20.584408358770613	24.14033792173832	29.887372065439777	25.38788165405129
36	29.38461168842007	23.42754610955017	26.387206417528837	20.80063578450092
37	21.699370537938428	29.30368446475493	27.105498751950474	21.891446245356168
38	21.674396902510516	30.599974204447456	24.09178158753923	23.633847305502798
39	21.815956319531026	28.45875367017604	25.693129025813256	24.032160984479674
40	23.32177169924409	24.495026769208202	26.583265261736344	25.599936269811362
41	18.861353963031434	23.5788420831679	27.00484551751695	30.55495843628372
42	23.479200439030187	26.458902879744674	23.399917049595743	26.661979631629396
43	22.482910446440087	26.28535191961904	25.0	26.231737633940877
44	21.247695470857348	28.872873447525016	27.529797656651077	22.34963342496655
45	20.255009268696085	30.976918038425254	23.912919778360568	24.85515291451809
46	23.559242521186494	28.88147196503944	26.44252776183118	21.116757751942885
47	23.014185024923055	25.407860562393626	27.138438660957924	24.439515751725395
48	23.361666291535265	22.6544382259741	28.926487733203178	25.057407749287464
49	21.058211963572635	22.85896913890905	30.722439854634477	25.360379042883842
50	19.681437570969383	28.389459734912766	28.04994474188009	23.879157952237765
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	351.0
1	911.5
2	1472.0
3	4502.0
4	7532.0
5	5496.0
6	3460.0
7	3717.5
8	3975.0
9	4224.5
10	4474.0
11	4524.5
12	4575.0
13	4445.0
14	4315.0
15	4075.5
16	3836.0
17	3823.5
18	3811.0
19	3944.0
20	4077.0
21	4146.0
22	4215.0
23	4825.0
24	5435.0
25	6774.5
26	8114.0
27	10154.5
28	12195.0
29	14831.5
30	17468.0
31	19768.0
32	22068.0
33	26518.5
34	30969.0
35	34875.0
36	38781.0
37	44521.0
38	50261.0
39	67897.5
40	85534.0
41	106459.5
42	127385.0
43	137908.0
44	148431.0
45	151118.0
46	153805.0
47	152354.0
48	150903.0
49	147233.0
50	143563.0
51	130113.0
52	116663.0
53	104515.0
54	92367.0
55	84028.0
56	75689.0
57	70867.5
58	66046.0
59	62560.5
60	59075.0
61	52984.0
62	46893.0
63	39119.5
64	31346.0
65	25277.5
66	19209.0
67	16212.5
68	13216.0
69	11049.5
70	8883.0
71	7325.5
72	5768.0
73	4395.0
74	3022.0
75	2137.0
76	1252.0
77	911.5
78	571.0
79	489.5
80	408.0
81	294.5
82	181.0
83	116.5
84	52.0
85	34.0
86	16.0
87	10.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1581668.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.588634018392845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.84293342205727	17.89031199374244
2	6.596576435550217	3.1120845462509696
3	2.3881909328724404	1.6900248564471656
4	1.4271421063444119	1.3465733135518647
5	1.0615776226037443	1.2520583010857642
6	0.7942830704786911	1.1241631593916308
7	0.6721921327562256	1.1099265946740677
8	0.582780029324328	1.0997587859967861
9	0.5007051066276086	1.0629854560241123
>10	9.282198967213219	55.0635372125617
>50	0.7764837107545003	11.343220580143571
>100	0.0692553597417802	2.621225355389826
>500	0.004328459943300546	0.6891277738581666
>1k	0.0013526437322814206	0.5950020708818915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2671	0.16887235500749842	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2160	0.13656468993492946	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2097	0.13258155314516068	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03597467989489577	0.0
2	0.0	0.0	0.0	0.12606944061585618	0.0
3	0.0	0.0	0.0	0.18550037049494583	0.0
4	0.0	0.0	0.0	0.309799528093127	0.0
5	0.0	0.0	0.0	0.5698414585108885	0.0
6	0.0	0.0	0.0	0.8461952824486555	0.0
7	6.322439348839327E-5	0.0	0.0	1.0266377014645298	0.0
8	6.322439348839327E-5	0.0	0.0	1.5627805582461047	0.0
9	6.322439348839327E-5	0.0	0.0	1.984676935994153	0.0
10	6.322439348839327E-5	0.0	0.0	2.6173634416325045	0.0
11	6.322439348839327E-5	0.0	0.0	3.049122824764742	0.0
12	6.322439348839327E-5	0.0	0.0	3.5152762779546656	0.0
13	6.322439348839327E-5	0.0	0.0	3.6861718135537926	0.0
14	6.322439348839327E-5	0.0	0.0	3.7444647043500914	0.0
15	6.322439348839327E-5	0.0	0.0	3.828995718444073	0.0
16	6.322439348839327E-5	0.0	0.0	4.008742669131575	0.0
17	6.322439348839327E-5	0.0	0.0	4.225728787583741	0.0
18	6.322439348839327E-5	0.0	0.0	4.54804674558757	0.0
19	6.322439348839327E-5	0.0	0.0	4.70579160734111	0.0
20	6.322439348839327E-5	0.0	0.0	4.885917904389543	0.0
21	6.322439348839327E-5	0.0	0.0	5.08709792446961	0.0
22	6.322439348839327E-5	0.0	0.0	5.299531886590612	0.0
23	6.322439348839327E-5	0.0	0.0	5.529542230101387	0.0
24	6.322439348839327E-5	0.0	0.0	5.68836190654423	0.0
25	6.322439348839327E-5	0.0	0.0	5.829162630842882	0.0
26	6.322439348839327E-5	0.0	0.0	5.972618779668046	0.0
27	6.322439348839327E-5	0.0	0.0	6.108298328094139	0.0
28	6.322439348839327E-5	0.0	0.0	6.255927286889537	0.0
29	6.322439348839327E-5	0.0	0.0	6.411143172903542	0.0
30	6.322439348839327E-5	0.0	0.0	6.61270253934454	0.0
31	6.322439348839327E-5	0.0	0.0	6.803007963744604	0.0
32	6.322439348839327E-5	0.0	0.0	6.965494655009774	0.0
33	6.322439348839327E-5	0.0	0.0	7.145684176451695	0.0
34	6.322439348839327E-5	0.0	0.0	7.321447990349428	0.0
35	6.322439348839327E-5	0.0	0.0	7.545578465265783	0.0
36	6.322439348839327E-5	0.0	0.0	7.727601494118867	0.0
37	6.322439348839327E-5	0.0	0.0	7.911521254776603	0.0
38	6.322439348839327E-5	0.0	0.0	8.10283826947248	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTCG	30	2.5283225E-6	44.000004	36
TAATCGG	30	2.5283225E-6	44.000004	17
ACGTTAG	25	4.4436027E-5	44.0	1
CGTACGT	20	7.8573823E-4	44.0	33
CACGTTA	20	7.8573823E-4	44.0	20
TAAGACG	25	4.4436027E-5	44.0	1
TAGTCCG	20	7.8573823E-4	44.0	2
GTACGTA	20	7.8573823E-4	44.0	11
CGTTTTT	3180	0.0	41.786163	1
TATGCGA	95	0.0	41.68421	2
CGTAAGA	85	0.0	41.411762	2
AGCGATC	80	0.0	41.25	8
ATTGCGA	140	0.0	40.857143	2
CGGTCTA	380	0.0	40.526314	31
GAGCGAT	915	0.0	40.153004	7
AGGGGAA	14070	0.0	39.65316	8
TCACGAC	395	0.0	39.544304	25
AGACTTA	1105	0.0	39.42082	8
CGACGGT	380	0.0	39.36842	28
CACGACG	375	0.0	39.306667	26
>>END_MODULE