FastQCFastQC Report
Thu 26 May 2016
SRR1546461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546461_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1411654
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82600.5851292172161168No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76090.539013100943999No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAA207.857094E-444.031
CGACGTC454.802132E-1044.018
GCGAATG600.044.01
CGCATCG254.443359E-544.021
CGGTCTA1100.044.031
CGTTTTT95200.043.26051
CGACGGT1200.040.33333228
GAGCGAT8750.040.2285737
AGCGATA2150.039.906988
ACGTCGC501.3478711E-939.620
GTTTTTA56850.038.737032
GGTACGA404.1254316E-738.52
TGCGCGA750.038.133332
TGTACGA750.038.133332
TATAGCG1100.038.01
GCGCGAC3850.037.7142879
TATACGA700.037.7142872
TCGTAAG1250.036.961
CTTGCGA900.036.6666682
ACGAGAT3500.036.457145
Produced by FastQC (version 0.11.5)