##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546461_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1411654 Sequences flagged as poor quality 0 Sequence length 50 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.601313778022092 31.0 31.0 34.0 30.0 34.0 2 31.982234315207553 31.0 31.0 34.0 30.0 34.0 3 32.0679932901405 33.0 31.0 34.0 30.0 34.0 4 35.758340924900864 37.0 35.0 37.0 35.0 37.0 5 35.71681020986729 37.0 35.0 37.0 33.0 37.0 6 35.768740782089665 37.0 35.0 37.0 35.0 37.0 7 36.08599982715311 37.0 35.0 37.0 35.0 37.0 8 18.180969274340598 23.0 0.0 37.0 0.0 37.0 9 27.805406282275968 22.0 17.0 39.0 17.0 39.0 10 33.70399049625475 32.0 32.0 39.0 27.0 39.0 11 35.85726105688788 37.0 35.0 39.0 32.0 39.0 12 36.64493282348224 38.0 35.0 39.0 33.0 39.0 13 36.869513351005274 39.0 35.0 39.0 33.0 39.0 14 38.077439655893016 40.0 37.0 41.0 33.0 41.0 15 38.29917387688484 40.0 37.0 41.0 33.0 41.0 16 38.35214153043168 40.0 37.0 41.0 34.0 41.0 17 38.310899129673416 40.0 37.0 41.0 34.0 41.0 18 38.26008993705256 40.0 37.0 41.0 34.0 41.0 19 38.249518649754116 40.0 37.0 41.0 34.0 41.0 20 38.138067826818755 40.0 36.0 41.0 34.0 41.0 21 38.02424815145921 40.0 36.0 41.0 33.0 41.0 22 38.01896711233773 40.0 36.0 41.0 33.0 41.0 23 37.87471575896077 39.0 36.0 41.0 33.0 41.0 24 37.704803726692234 39.0 35.0 41.0 33.0 41.0 25 37.57787248150042 39.0 35.0 41.0 33.0 41.0 26 37.53586431235983 39.0 35.0 41.0 33.0 41.0 27 37.40677460624204 39.0 35.0 41.0 33.0 41.0 28 37.38009526413696 39.0 35.0 41.0 33.0 41.0 29 37.26905955708693 39.0 35.0 41.0 33.0 41.0 30 37.14704382235307 39.0 35.0 41.0 32.0 41.0 31 37.12537774837177 39.0 35.0 41.0 32.0 41.0 32 36.86865903401258 39.0 35.0 41.0 31.0 41.0 33 36.685888326742955 40.0 35.0 41.0 31.0 41.0 34 36.49711756563577 40.0 35.0 41.0 30.0 41.0 35 36.30219940580341 40.0 35.0 41.0 30.0 41.0 36 36.16835357672631 39.0 35.0 41.0 29.0 41.0 37 36.1176662269933 39.0 35.0 41.0 29.0 41.0 38 36.03507233358883 39.0 35.0 41.0 28.0 41.0 39 35.97346941956032 39.0 35.0 41.0 28.0 41.0 40 35.9017393780629 39.0 35.0 41.0 27.0 41.0 41 35.88228631095155 39.0 35.0 41.0 27.0 41.0 42 35.74817200248786 39.0 35.0 41.0 27.0 41.0 43 35.764402608571224 39.0 35.0 41.0 27.0 41.0 44 35.53883104500111 39.0 35.0 40.0 26.0 41.0 45 35.45810800663619 39.0 35.0 40.0 26.0 41.0 46 35.45001749720541 38.0 35.0 40.0 26.0 41.0 47 35.35164353304705 38.0 35.0 40.0 25.0 41.0 48 35.34142785696778 38.0 35.0 40.0 25.0 41.0 49 35.29803124561684 38.0 35.0 40.0 26.0 41.0 50 34.703022128651924 38.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 4.0 13 6.0 14 6.0 15 17.0 16 47.0 17 149.0 18 315.0 19 802.0 20 1465.0 21 2589.0 22 4137.0 23 6539.0 24 10166.0 25 15359.0 26 21296.0 27 24631.0 28 24962.0 29 25812.0 30 29494.0 31 36643.0 32 47746.0 33 63417.0 34 95686.0 35 142851.0 36 143261.0 37 223748.0 38 257138.0 39 233365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.464258238916905 22.941244809280462 27.827144611923316 16.76735233987932 2 34.819863790985615 23.762621718919792 28.79728318695658 12.620231303138022 3 28.293477013489145 23.832468862766657 35.22258287087346 12.651471252870747 4 25.275173661534627 26.833841720421574 34.01725918674123 13.873725431302573 5 21.865981324035495 31.46238384193294 32.82631579693041 13.84531903710116 6 20.185257860637236 39.913534052961985 29.581398841359142 10.319809245041633 7 85.08090509430781 3.172094578416524 9.980136775725496 1.7668635515501674 8 43.29694103512617 50.8417076705765 4.845663314098214 1.0156879801991139 9 81.22266504398387 4.905451335808916 11.19056085981409 2.6813227603931273 10 39.33733053566951 34.64106643695977 15.925361313749686 10.096241713621042 11 30.72920134820572 23.150361207491354 31.04238007330408 15.078057370998843 12 29.87693868327508 22.133256449526584 30.82880082513137 17.161004042066967 13 21.939795445626196 27.95068763308856 31.976036620871685 18.133480300413556 14 16.953729454951425 31.556457885572524 32.793375713878895 18.69643694559715 15 17.1864352029605 25.708920174490352 40.05209491844319 17.052549704105964 16 19.55422504381385 23.63461584779273 39.00566286072933 17.805496247664088 17 19.380457250856086 23.35274791131538 32.34376128994782 24.923033547880713 18 21.08972878623232 25.06605726332373 33.8684975213473 19.97571642909665 19 23.887227323409277 26.64463104981816 29.992335232287797 19.475806394484767 20 26.332727424708885 24.96766204749889 29.700337334786003 18.999273193006218 21 23.256548701027306 26.638538905425836 31.24611271600548 18.85879967754138 22 21.79485908019954 23.685619847356364 31.857877355215937 22.66164371722816 23 20.40159982545298 27.64799306345606 31.233220038338004 20.717187072752953 24 20.445165741746916 24.623101694891243 35.4846867575199 19.44704580584194 25 19.88369671321726 26.659365538580982 32.81583164146455 20.641106106737205 26 19.083217275621365 30.103552286891833 30.471135278191397 20.342095159295408 27 19.67996407051586 27.7529762958912 32.075352742244206 20.49170689134873 28 18.62496050731978 27.196253472876496 35.42999913576556 18.748786884038157 29 19.440032755901942 25.313568338983917 34.651125559095924 20.595273346018217 30 20.717682944970935 26.46987151242443 32.99597493436777 19.816470608236862 31 24.636561083664976 25.587289803308742 29.313982038091485 20.462167074934793 32 24.617930456046594 27.57297468076455 29.06866696796807 18.740427895220783 33 23.960403895005435 26.721137049163605 29.546546108323994 19.771912947506966 34 20.543985990901454 27.457223937310417 30.659495882135424 21.339294189652705 35 20.68623047857336 26.43395619606504 31.18738727761902 21.69242604774258 36 24.272874231220964 27.130940017879734 28.574849077748514 20.021336673150785 37 21.67485800344844 28.483183556310543 29.586782596868638 20.255175843372385 38 20.905618515585264 29.22493755551998 28.207266086448946 21.66217784244581 39 21.28000204015998 27.471675070520114 28.794024598095568 22.454298291224337 40 22.41894968597121 25.70041950789641 29.775355717477513 22.105275088654867 41 19.04850622036278 26.013456555218205 30.302892918519692 24.63514430589932 42 21.330581006393917 27.47507533715769 27.363787443665373 23.83055621278302 43 21.476863310697947 27.178472911917513 28.544317516898616 22.800346260485927 44 21.29254052338604 28.32627541876409 29.230392149917755 21.150791907932113 45 20.55043232973519 28.943282135707477 27.78428708451221 22.721998450045124 46 21.611032165105616 29.1757753670517 28.355036007406913 20.858156460435772 47 21.584255065334705 26.582363667017557 29.303639560402196 22.52974170724554 48 21.646097414805613 25.25045088952392 30.573143277318664 22.530308418351805 49 21.193224402013524 25.266956350493817 30.76426659790572 22.77555264958694 50 19.40362156732457 27.60102688052455 30.57555179952028 22.419799752630603 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 382.0 1 899.0 2 1416.0 3 13889.0 4 26362.0 5 18425.0 6 10488.0 7 10437.0 8 10386.0 9 10496.0 10 10606.0 11 10332.0 12 10058.0 13 9692.5 14 9327.0 15 8893.5 16 8460.0 17 7952.5 18 7445.0 19 7283.0 20 7121.0 21 7475.0 22 7829.0 23 8751.5 24 9674.0 25 11613.5 26 13553.0 27 16520.5 28 19488.0 29 23024.5 30 26561.0 31 30370.5 32 34180.0 33 38134.0 34 42088.0 35 46832.0 36 51576.0 37 57092.0 38 62608.0 39 71846.5 40 81085.0 41 91073.5 42 101062.0 43 106380.5 44 111699.0 45 114539.0 46 117379.0 47 117962.0 48 118545.0 49 114000.5 50 109456.0 51 98245.5 52 87035.0 53 78190.0 54 69345.0 55 64047.5 56 58750.0 57 55686.5 58 52623.0 59 48967.0 60 45311.0 61 40056.0 62 34801.0 63 28432.5 64 22064.0 65 17704.0 66 13344.0 67 10710.0 68 8076.0 69 6532.0 70 4988.0 71 4158.5 72 3329.0 73 2449.5 74 1570.0 75 1235.0 76 900.0 77 675.5 78 451.0 79 293.0 80 135.0 81 99.0 82 63.0 83 40.0 84 17.0 85 11.5 86 6.0 87 5.5 88 5.0 89 5.5 90 6.0 91 3.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1411654.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.58681784950925 #Duplication Level Percentage of deduplicated Percentage of total 1 69.7964801480893 23.44241665270764 2 7.073089145412427 4.751251135206165 3 3.928321604211034 3.9581946652478397 4 2.718709139880072 3.652511546677918 5 2.236972773331452 3.7566398536097507 6 1.8472487235428225 3.7225923840222754 7 1.6134851370216223 3.7934281980024998 8 1.4154351536344125 3.8031970146328926 9 1.2216690345928773 3.692877780353111 >10 8.097192564893021 42.32353552420577 >50 0.03256958134366133 0.708304094832919 >100 0.017543338351070404 1.0655618775608486 >500 8.557704641587292E-4 0.1891982086072868 >1k 0.0 0.0 >5k 4.278852320793646E-4 1.1402910643330932 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8260 0.5851292172161168 No Hit CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7609 0.539013100943999 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.030956594179593584 0.0 2 0.0 0.0 0.0 0.09095713255514454 0.0 3 0.0 0.0 0.0 0.16321279860362384 0.0 4 0.0 0.0 0.0 0.23419336466301233 0.0 5 0.0 0.0 0.0 0.3981145521494644 0.0 6 0.0 0.0 0.0 0.7151185772150966 0.0 7 0.0 0.0 0.0 0.9147425643960914 0.0 8 0.0 0.0 0.0 1.3258206331013123 0.0 9 0.0 0.0 0.0 1.763463284912592 0.0 10 0.0 0.0 0.0 2.286466797104673 0.0 11 0.0 0.0 0.0 2.646470027357979 0.0 12 0.0 0.0 0.0 2.971408007911287 0.0 13 0.0 0.0 0.0 3.1480093564003644 0.0 14 0.0 0.0 0.0 3.2522841999526797 0.0 15 0.0 0.0 0.0 3.3354490547967135 0.0 16 0.0 0.0 0.0 3.4830772979781166 0.0 17 0.0 0.0 0.0 3.6418272466199224 0.0 18 0.0 0.0 0.0 3.883529533440914 0.0 19 0.0 0.0 0.0 4.005443260175652 0.0 20 0.0 0.0 0.0 4.137416109046551 0.0 21 0.0 0.0 0.0 4.282848346691186 0.0 22 0.0 0.0 0.0 4.46086647294592 0.0 23 0.0 0.0 0.0 4.643914160268735 0.0 24 0.0 0.0 0.0 4.792179953444682 0.0 25 0.0 0.0 0.0 4.915510457945077 0.0 26 0.0 0.0 0.0 5.044933106837795 0.0 27 0.0 0.0 0.0 5.184202361201824 0.0 28 0.0 0.0 0.0 5.331476409941813 0.0 29 0.0 0.0 0.0 5.480733947553721 0.0 30 0.0 0.0 0.0 5.678091090309665 0.0 31 0.0 0.0 0.0 5.837266072281168 0.0 32 0.0 0.0 0.0 6.0067126930536805 0.0 33 0.0 0.0 0.0 6.1705630416518495 0.0 34 0.0 0.0 0.0 6.341851473519715 0.0 35 0.0 0.0 0.0 6.525890905278489 0.0 36 0.0 0.0 0.0 6.695550042715849 0.0 37 7.083888828282284E-5 0.0 0.0 6.880793735575431 0.0 38 7.083888828282284E-5 0.0 0.0 7.0791426227673355 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCGAA 20 7.857094E-4 44.0 31 CGACGTC 45 4.802132E-10 44.0 18 GCGAATG 60 0.0 44.0 1 CGCATCG 25 4.443359E-5 44.0 21 CGGTCTA 110 0.0 44.0 31 CGTTTTT 9520 0.0 43.2605 1 CGACGGT 120 0.0 40.333332 28 GAGCGAT 875 0.0 40.228573 7 AGCGATA 215 0.0 39.90698 8 ACGTCGC 50 1.3478711E-9 39.6 20 GTTTTTA 5685 0.0 38.73703 2 GGTACGA 40 4.1254316E-7 38.5 2 TGCGCGA 75 0.0 38.13333 2 TGTACGA 75 0.0 38.13333 2 TATAGCG 110 0.0 38.0 1 GCGCGAC 385 0.0 37.714287 9 TATACGA 70 0.0 37.714287 2 TCGTAAG 125 0.0 36.96 1 CTTGCGA 90 0.0 36.666668 2 ACGAGAT 350 0.0 36.45714 5 >>END_MODULE