FastQCFastQC Report
Thu 26 May 2016
SRR1546460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546460_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1366975
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45400.332120192395618No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42450.31053969531264286No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA207.857005E-444.0000042
ACTACGC207.857005E-444.00000436
TCGACAG408.314601E-944.0000041
TAGTACG207.857005E-444.0000041
TTGTACG454.802132E-1044.01
TATGCGA1050.044.02
ACTCGAT302.5280697E-644.012
CGTTTTT65400.042.8562661
CGACGGT1950.040.61538728
TATAGCG650.040.6153871
GAGCGAT8200.040.5121967
GCGTAAG1200.040.3333321
GCGTAAC603.6379788E-1240.33333232
AGCGATA2150.039.9069758
CGGTCTA2000.039.631
TCGTAGA452.3515895E-839.111112
CCGGTCA452.3515895E-839.1111132
GTTTTTA37650.039.0916332
GACCGAT4800.038.9583329
GTATGCG850.038.823531