##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546460_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1366975
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56324146381609	31.0	31.0	34.0	30.0	34.0
2	31.939452440607912	31.0	31.0	34.0	30.0	34.0
3	32.02982863622231	33.0	31.0	34.0	30.0	34.0
4	35.71466120448435	37.0	35.0	37.0	35.0	37.0
5	35.659356608570015	37.0	35.0	37.0	33.0	37.0
6	35.71406207136195	37.0	35.0	37.0	33.0	37.0
7	36.070260246163976	37.0	35.0	37.0	35.0	37.0
8	18.505070685272226	30.0	0.0	37.0	0.0	37.0
9	27.976854002450665	30.0	17.0	39.0	17.0	39.0
10	33.697800618153224	32.0	32.0	39.0	27.0	39.0
11	35.810132592037164	37.0	35.0	39.0	32.0	39.0
12	36.48234532453044	37.0	35.0	39.0	33.0	39.0
13	36.61314142541012	39.0	35.0	39.0	33.0	39.0
14	37.76902357394978	39.0	36.0	41.0	33.0	41.0
15	38.025497174417964	40.0	36.0	41.0	33.0	41.0
16	38.11030267561587	40.0	36.0	41.0	33.0	41.0
17	38.05962947383822	40.0	36.0	41.0	33.0	41.0
18	38.027583532983414	40.0	36.0	41.0	33.0	41.0
19	38.030018837213554	40.0	36.0	41.0	34.0	41.0
20	37.92678871230271	40.0	36.0	41.0	33.0	41.0
21	37.814109987380895	40.0	36.0	41.0	33.0	41.0
22	37.798819290769764	40.0	36.0	41.0	33.0	41.0
23	37.69073757749776	39.0	36.0	41.0	33.0	41.0
24	37.5646986960259	39.0	35.0	41.0	33.0	41.0
25	37.435014539402694	39.0	35.0	41.0	33.0	41.0
26	37.3911373653505	39.0	35.0	41.0	33.0	41.0
27	37.247281771795386	39.0	35.0	41.0	32.0	41.0
28	37.25465279174821	39.0	35.0	41.0	32.0	41.0
29	37.166985497174416	39.0	35.0	41.0	32.0	41.0
30	37.063206715558074	39.0	35.0	41.0	32.0	41.0
31	37.06655425300389	39.0	35.0	41.0	32.0	41.0
32	36.87275699994513	39.0	35.0	41.0	31.0	41.0
33	36.739442199016075	39.0	35.0	41.0	31.0	41.0
34	36.62005011064577	39.0	35.0	41.0	31.0	41.0
35	36.451673220066205	39.0	35.0	41.0	30.0	41.0
36	36.32298615556247	39.0	35.0	41.0	30.0	41.0
37	36.257319994879204	39.0	35.0	41.0	30.0	41.0
38	36.20084712595329	39.0	35.0	41.0	30.0	41.0
39	36.14132006803343	39.0	35.0	41.0	30.0	41.0
40	36.06635015270945	39.0	35.0	41.0	29.0	41.0
41	36.07200277986064	39.0	35.0	41.0	29.0	41.0
42	35.92722690612484	39.0	35.0	40.0	29.0	41.0
43	35.93418533623512	39.0	35.0	40.0	29.0	41.0
44	35.70048318367198	38.0	35.0	40.0	28.0	41.0
45	35.61027012198468	38.0	35.0	40.0	27.0	41.0
46	35.611002395800945	38.0	35.0	40.0	28.0	41.0
47	35.49231990343642	38.0	35.0	40.0	27.0	41.0
48	35.496886190310725	38.0	35.0	40.0	27.0	41.0
49	35.45560964904259	38.0	35.0	40.0	27.0	41.0
50	34.831829404341704	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	9.0
15	16.0
16	43.0
17	118.0
18	327.0
19	772.0
20	1505.0
21	2425.0
22	3991.0
23	6022.0
24	8902.0
25	13287.0
26	17905.0
27	20330.0
28	21848.0
29	24529.0
30	29124.0
31	37637.0
32	48976.0
33	65977.0
34	100560.0
35	155686.0
36	139087.0
37	215182.0
38	243254.0
39	209458.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.743082353371495	24.0858830629675	29.636240604253917	14.534793979407084
2	32.49240110462883	24.751513378079334	30.11393770917537	12.64214780811646
3	29.10228789846193	24.36789260959418	33.50185628852027	13.027963203423617
4	26.064412297225626	27.304449605881597	32.376305345745166	14.254832751147607
5	22.729749995427863	31.982516139651416	30.995519303571754	14.292214561348965
6	20.0844199784195	40.94186067777392	28.28910550668447	10.684613837122113
7	87.15923846449277	3.372921962727921	7.834232520711791	1.6336070520675212
8	45.12416101245451	50.04641635728525	3.654126812853198	1.1752958174070485
9	83.34431865981455	4.768850929973116	9.067539640446972	2.8192907697653578
10	43.57043837670769	32.29532361601346	13.657455330199895	10.476782677078953
11	35.89890085773332	22.342471515572708	26.577150277071638	15.181477349622341
12	34.094405530459596	21.60171180892116	27.38323670879131	16.920645951827943
13	22.9548455531374	30.586952943543228	28.916183543956546	17.542017959362827
14	17.508586477441064	33.898571663710015	30.385925126648257	18.20691673220066
15	17.06088260575358	24.4332193346623	41.37573840048282	17.130159659101302
16	20.02618921340917	21.42760474770936	39.69275224492036	18.85345379396112
17	19.491651273797984	22.170266464273304	30.325499734815924	28.012582527112784
18	22.05358547157044	24.447338100550486	32.6985497174418	20.80052671043728
19	26.509409462499313	26.12008266427696	27.170869986649354	20.19963788657437
20	28.217121746922952	24.699427568170595	27.1326103257192	19.950840359187254
21	23.966787980760436	27.27906508897383	28.58867206788712	20.16547486237861
22	22.49565646774813	23.81887013295781	28.97858409992867	24.706889299365386
23	20.87602187311399	28.41888110609192	28.411126757987525	22.29397026280656
24	21.249766820900163	24.6959893194828	34.555057700396866	19.499186159220177
25	20.333363814261414	25.823295963715502	32.16788895188281	21.67545127014027
26	19.49735730353518	30.371586898077872	28.81325554600486	21.317800252382085
27	20.40754220084493	28.007315422739993	30.98213207995757	20.60301029645751
28	18.45000822985058	27.244609447868466	35.43641983211105	18.8689624901699
29	20.03555295451636	24.896578211013367	33.74765449258399	21.32021434188628
30	21.682254613288464	26.70268293128989	30.861354450520313	20.753708004901334
31	26.977011284039577	25.631705042155122	26.413284807695824	20.977998866109477
32	28.494083651859032	26.481464547632545	26.142760474770938	18.88169132573749
33	26.33296146601072	26.951626767131803	26.685345379396114	20.030066387461364
34	22.12915378847455	26.621115967738984	29.086852356480552	22.162877887305914
35	22.16690136981291	25.935807165456577	29.874869694032448	22.022421770698074
36	26.333985625194316	27.18411090180874	26.69010040417711	19.79180306881984
37	22.389290221108652	29.84253552552168	28.13489639532544	19.63327785804422
38	21.847144241847875	30.464492766875768	25.50273413924907	22.185628852027286
39	22.196089906545474	29.289709029060518	26.989154885787965	21.525046178606047
40	23.395234002084894	25.66030834506849	27.40145211141389	23.543005541432727
41	19.073209093070464	25.43462755353975	27.986759084840617	27.505404268549167
42	22.093308217048595	27.366777007626332	25.10199528155233	25.43791949377275
43	21.741216920572796	27.460341264470824	26.46010351323177	24.33833830172461
44	21.569085023500794	28.336289983357414	28.00673018892079	22.087894804220998
45	20.693867846888203	30.32096417271713	25.86623749519925	23.118930485195413
46	22.737431189304854	29.222626602534795	27.101373470619432	20.938568737540923
47	22.352786261636094	26.381755335686464	27.99305034839701	23.272408054280437
48	22.51518864646391	24.56431171016295	29.733169955558807	23.187329687814334
49	21.80822619287112	24.07739717258911	30.321915177673333	23.79246145686644
50	20.121143400574258	28.220998920975145	28.717204045428772	22.940653633021817
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	360.0
1	806.0
2	1252.0
3	8374.0
4	15496.0
5	11132.0
6	6768.0
7	6749.0
8	6730.0
9	6934.5
10	7139.0
11	7099.5
12	7060.0
13	6854.5
14	6649.0
15	6287.0
16	5925.0
17	5822.0
18	5719.0
19	5714.0
20	5709.0
21	5983.5
22	6258.0
23	7001.5
24	7745.0
25	9280.0
26	10815.0
27	12892.0
28	14969.0
29	18208.0
30	21447.0
31	24478.5
32	27510.0
33	30635.5
34	33761.0
35	38027.5
36	42294.0
37	48194.5
38	54095.0
39	65912.0
40	77729.0
41	89349.0
42	100969.0
43	107607.0
44	114245.0
45	117379.5
46	120514.0
47	120725.0
48	120936.0
49	115642.0
50	110348.0
51	100621.5
52	90895.0
53	82809.5
54	74724.0
55	68658.5
56	62593.0
57	59176.0
58	55759.0
59	52492.5
60	49226.0
61	43779.5
62	38333.0
63	31693.0
64	25053.0
65	19973.0
66	14893.0
67	11967.5
68	9042.0
69	7425.0
70	5808.0
71	4813.0
72	3818.0
73	3020.0
74	2222.0
75	1697.0
76	1172.0
77	899.0
78	626.0
79	422.5
80	219.0
81	159.5
82	100.0
83	61.5
84	23.0
85	19.0
86	15.0
87	12.0
88	9.0
89	6.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1366975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.501955665616876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.1400847913791	22.004136680204876
2	6.689261467124469	4.080711134118998
3	3.2282756141273197	2.9540615897551086
4	2.1429465783981305	2.6145624611234064
5	1.7668647311839438	2.6946414848857367
6	1.3984924408062527	2.559405265690359
7	1.2094972639656847	2.582442234621636
8	1.0829418423802963	2.6425475251780206
9	0.9778150636830225	2.6842744549468707
>10	9.237968840825843	51.050079042783395
>50	0.10203793130556765	2.038737639109575
>100	0.021889117304917015	1.1528521396437512
>500	0.0012026984472097394	0.22040140553311358
>1k	7.216190683258436E-4	0.7211469424051176
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4540	0.332120192395618	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4245	0.31053969531264286	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02926169095996635	0.0
2	0.0	0.0	0.0	0.11602260465626657	0.0
3	0.0	0.0	0.0	0.1889573693739827	0.0
4	0.0	0.0	0.0	0.28427732767607305	0.0
5	0.0	0.0	0.0	0.48969439821503685	0.0
6	0.0	0.0	0.0	0.780848223266702	0.0
7	0.0	0.0	0.0	0.9923370946798589	0.0
8	0.0	0.0	0.0	1.4520382596609303	0.0
9	0.0	0.0	0.0	1.8671153459280527	0.0
10	0.0	0.0	0.0	2.4396203295597942	0.0
11	0.0	0.0	0.0	2.8553558038735165	0.0
12	0.0	0.0	0.0	3.285429506757622	0.0
13	7.315422739991587E-5	0.0	0.0	3.4666325280272132	0.0
14	7.315422739991587E-5	0.0	0.0	3.566414894200699	0.0
15	7.315422739991587E-5	0.0	0.0	3.6631247828233873	0.0
16	7.315422739991587E-5	0.0	0.0	3.837963386309186	0.0
17	7.315422739991587E-5	0.0	0.0	4.054646207867737	0.0
18	1.4630845479983174E-4	0.0	0.0	4.350042978108598	0.0
19	1.4630845479983174E-4	0.0	0.0	4.508129263519816	0.0
20	1.4630845479983174E-4	0.0	0.0	4.678871230271219	0.0
21	1.4630845479983174E-4	0.0	0.0	4.874266171656394	0.0
22	2.1946268219974761E-4	0.0	0.0	5.096362406042539	0.0
23	2.1946268219974761E-4	0.0	0.0	5.335430421185464	0.0
24	2.1946268219974761E-4	0.0	0.0	5.513926736041259	0.0
25	2.1946268219974761E-4	0.0	0.0	5.6704767826770786	0.0
26	2.1946268219974761E-4	0.0	0.0	5.822710729896304	0.0
27	2.1946268219974761E-4	0.0	0.0	5.979626547669123	0.0
28	2.1946268219974761E-4	0.0	0.0	6.145174564275133	0.0
29	2.1946268219974761E-4	0.0	0.0	6.319427933941733	0.0
30	2.1946268219974761E-4	0.0	0.0	6.538597999231881	0.0
31	2.1946268219974761E-4	0.0	0.0	6.722727189597469	0.0
32	2.1946268219974761E-4	0.0	0.0	6.902759743228662	0.0
33	2.1946268219974761E-4	0.0	0.0	7.091131878783445	0.0
34	2.1946268219974761E-4	0.0	0.0	7.279942939702628	0.0
35	2.1946268219974761E-4	0.0	0.0	7.501015014905174	0.0
36	2.1946268219974761E-4	0.0	0.0	7.700579747252144	0.0
37	2.1946268219974761E-4	0.0	0.0	7.915287404670897	0.0
38	2.1946268219974761E-4	0.0	0.0	8.141626584246238	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	20	7.857005E-4	44.000004	2
ACTACGC	20	7.857005E-4	44.000004	36
TCGACAG	40	8.314601E-9	44.000004	1
TAGTACG	20	7.857005E-4	44.000004	1
TTGTACG	45	4.802132E-10	44.0	1
TATGCGA	105	0.0	44.0	2
ACTCGAT	30	2.5280697E-6	44.0	12
CGTTTTT	6540	0.0	42.856266	1
CGACGGT	195	0.0	40.615387	28
TATAGCG	65	0.0	40.615387	1
GAGCGAT	820	0.0	40.512196	7
GCGTAAG	120	0.0	40.333332	1
GCGTAAC	60	3.6379788E-12	40.333332	32
AGCGATA	215	0.0	39.906975	8
CGGTCTA	200	0.0	39.6	31
TCGTAGA	45	2.3515895E-8	39.11111	2
CCGGTCA	45	2.3515895E-8	39.11111	32
GTTTTTA	3765	0.0	39.091633	2
GACCGAT	480	0.0	38.958332	9
GTATGCG	85	0.0	38.82353	1
>>END_MODULE