FastQCFastQC Report
Thu 26 May 2016
SRR1546459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546459_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1667992
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81400.4880119329109492No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76180.45671681878570164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA351.4466059E-744.02
ACGTCGA207.857506E-444.02
CCACGAC207.857506E-444.018
GTTGCGA502.7284841E-1144.02
CGTTTTT113650.043.070831
GAGCGAT10550.042.1232227
TATCGCG700.040.8571431
TATAGCG1250.040.481
TGCGCGA603.6379788E-1240.3333322
TATTGCG1000.039.6000021
ACGAGAT3450.039.5362325
CGGTCTA2400.039.41666431
TATGCGA900.039.111112
GTTTTTA68200.038.645162
CGACGGT2450.038.61224428
TTTCGCG404.1260319E-738.51
AGCGATA2400.038.58
GCGATGT2900.037.9310389
TCACGAC2500.037.8425
CGTATTG357.292183E-637.71428744
Produced by FastQC (version 0.11.5)