##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546459_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1667992
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62574400836455	31.0	31.0	34.0	30.0	34.0
2	31.999627696056095	31.0	31.0	34.0	30.0	34.0
3	32.084096926124346	33.0	31.0	34.0	30.0	34.0
4	35.76909661437225	37.0	35.0	37.0	35.0	37.0
5	35.7155669811366	37.0	35.0	37.0	33.0	37.0
6	35.768058240087484	37.0	35.0	37.0	35.0	37.0
7	36.11786147655384	37.0	35.0	37.0	35.0	37.0
8	18.105847030441392	10.0	0.0	37.0	0.0	37.0
9	27.77949474577816	19.0	17.0	39.0	17.0	39.0
10	33.6506224250476	32.0	32.0	39.0	27.0	39.0
11	35.83487330874489	37.0	35.0	39.0	32.0	39.0
12	36.621861495738585	37.0	35.0	39.0	33.0	39.0
13	36.824975779260335	39.0	35.0	39.0	33.0	39.0
14	38.02139338797788	40.0	37.0	41.0	33.0	41.0
15	38.26326265353791	40.0	37.0	41.0	33.0	41.0
16	38.334048964263616	40.0	37.0	41.0	34.0	41.0
17	38.2921338951266	40.0	37.0	41.0	34.0	41.0
18	38.2427859366232	40.0	37.0	41.0	34.0	41.0
19	38.24308509872949	40.0	37.0	41.0	34.0	41.0
20	38.12420862929798	40.0	36.0	41.0	34.0	41.0
21	38.021295665686644	40.0	36.0	41.0	33.0	41.0
22	38.01189813860019	40.0	36.0	41.0	33.0	41.0
23	37.87550599763068	39.0	36.0	41.0	33.0	41.0
24	37.72580204221603	39.0	35.0	41.0	33.0	41.0
25	37.622836320557894	39.0	35.0	41.0	33.0	41.0
26	37.576994973596996	39.0	35.0	41.0	33.0	41.0
27	37.43892896368808	39.0	35.0	41.0	33.0	41.0
28	37.42850864992158	39.0	35.0	41.0	33.0	41.0
29	37.33975642569029	39.0	35.0	41.0	33.0	41.0
30	37.233222941117226	39.0	35.0	41.0	33.0	41.0
31	37.20291464227646	39.0	35.0	41.0	32.0	41.0
32	36.93956625691251	39.0	35.0	41.0	31.0	41.0
33	36.762178115962186	40.0	35.0	41.0	31.0	41.0
34	36.583220423119535	40.0	35.0	41.0	30.0	41.0
35	36.392064230523886	40.0	35.0	41.0	30.0	41.0
36	36.24314744914844	39.0	35.0	41.0	30.0	41.0
37	36.16140065419978	39.0	35.0	41.0	29.0	41.0
38	36.08720485469954	39.0	35.0	41.0	29.0	41.0
39	36.022239315296474	39.0	35.0	41.0	28.0	41.0
40	35.94977553849179	39.0	35.0	41.0	28.0	41.0
41	35.96557357589245	39.0	35.0	41.0	28.0	41.0
42	35.82194399013904	39.0	35.0	41.0	27.0	41.0
43	35.83997225406357	39.0	35.0	41.0	27.0	41.0
44	35.63720329593907	39.0	35.0	40.0	26.0	41.0
45	35.5709403882033	39.0	35.0	40.0	26.0	41.0
46	35.58528817884019	39.0	35.0	40.0	26.0	41.0
47	35.49191782694401	39.0	35.0	40.0	26.0	41.0
48	35.49092141928738	39.0	35.0	40.0	26.0	41.0
49	35.45701238375244	39.0	35.0	40.0	26.0	41.0
50	34.8406197391834	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	2.0
13	2.0
14	8.0
15	16.0
16	55.0
17	135.0
18	353.0
19	812.0
20	1698.0
21	2910.0
22	4779.0
23	7731.0
24	12003.0
25	18535.0
26	24885.0
27	27812.0
28	27641.0
29	28783.0
30	33867.0
31	42639.0
32	56115.0
33	75254.0
34	113171.0
35	173709.0
36	166123.0
37	263303.0
38	306103.0
39	279546.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.0655015131967	23.30460817557878	28.72657662626679	15.90331368495772
2	34.05130240432808	24.390104988513134	29.333653878435868	12.22493872872292
3	27.584125103717522	24.504673883327975	35.111019717120946	12.800181295833553
4	24.808152557086604	26.760739859663595	34.74543043371911	13.685677149530692
5	22.524808272461737	31.345234269708726	32.562925961275596	13.567031496553941
6	19.378270399378415	40.35594894939544	29.838212653298097	10.427567997928048
7	85.71426002043175	3.0468971074201794	9.72522649988729	1.5136163722607783
8	43.46064009899328	51.07074854076039	4.512251857323057	0.9563595029232754
9	81.8029702780349	4.843548410304126	10.785783145242902	2.5676981664180643
10	39.72770852617998	34.059036254370525	16.67328140662545	9.539973812824043
11	30.852905769332228	22.755444870239184	31.808126178063205	14.583523182365383
12	29.228857212744426	22.585719835586744	31.551470270840625	16.633952680828205
13	21.22737998743399	30.797749629494625	31.77976872790757	16.195101655163814
14	16.663928843783424	33.564669374913066	32.87731595834992	16.894085822953585
15	16.298639322011137	26.097906944397813	41.92478141381973	15.678672319771318
16	18.912141065424773	23.66923822176605	39.923692679581194	17.49492803322798
17	18.84529422203464	23.68428625556957	32.64673931289838	24.823680209497407
18	20.938889395152973	24.977218116154035	35.07318979947146	19.01070268922153
19	23.773315459546566	26.570690986527513	30.882462266006073	18.773531287919845
20	25.123861505330964	25.780639235679786	30.665614703188027	18.429884555801227
21	22.02180825807318	27.24167741811711	32.103451335498015	18.633062988311693
22	21.08565268898172	24.337406894037862	32.29835634703284	22.278584069947577
23	18.648290879092947	28.421359335056763	31.908906037918648	21.021443747931645
24	18.54715130528204	25.641070220960295	37.457074134648124	18.35470433910954
25	18.019510884944292	27.460563360016117	34.80370409450405	19.716221660535542
26	17.94942661595499	31.043913879682876	31.527609245128275	19.47905025923386
27	18.38629921486434	29.274121218806805	32.838586755811775	19.500992810517076
28	17.04708415867702	27.84473786445019	37.0225996287752	18.08557834809759
29	18.014175127938266	24.911090700674823	36.738245746982	20.336488424404912
30	19.118496971208497	27.498453229991508	33.82144518678747	19.56160461201253
31	23.199631652909606	26.48969539422251	29.860155204581318	20.45051774828656
32	24.260488059894772	26.596050820387628	30.48293996613893	18.660521153578674
33	23.17858838651504	27.895397579844506	29.743428026033698	19.18258600760675
34	19.32539244792541	27.922376126504204	31.130544990623456	21.62168643494693
35	20.263466491446003	27.359423786205213	31.716099357790682	20.661010364558102
36	24.082069937985313	27.758706276768713	29.20865327891261	18.950570506333364
37	21.051419910886864	30.576585499211028	29.444685586021997	18.927309003880115
38	20.782174015223095	30.75308514669135	28.283289128485027	20.181451709600527
39	21.524743523949756	29.560633384332778	28.7139866378256	20.200636453891864
40	22.486678593182702	26.873270375397485	29.16884493450808	21.471206096911736
41	19.447215574175416	26.385138537834713	29.41626818354045	24.751377704449425
42	21.38247665456429	27.73130806382764	27.856728329632276	23.029486951975787
43	21.003218240854874	27.76356241516746	29.058172940877412	22.175046403100254
44	20.471740871658856	29.020223118576112	29.685274269900574	20.82276173986446
45	19.390620578515964	30.912798142916753	28.043299967865554	21.65328131070173
46	20.952978191741927	30.064232921980445	28.996601902167395	19.986186984110237
47	20.517604400980343	27.309783260351367	30.110336260605568	22.062276078062727
48	21.508376538976208	25.343406922814975	31.216876339934487	21.93134019827433
49	21.054237670204653	25.102398572655026	31.580067530299903	22.263296226840417
50	19.5964968656924	28.924179492467587	30.05613935798253	21.423184283857477
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	395.0
1	946.0
2	1497.0
3	14199.0
4	26901.0
5	19105.0
6	11309.0
7	11295.0
8	11281.0
9	11521.0
10	11761.0
11	11709.0
12	11657.0
13	11329.5
14	11002.0
15	10506.0
16	10010.0
17	9541.5
18	9073.0
19	9232.5
20	9392.0
21	10169.0
22	10946.0
23	12367.5
24	13789.0
25	15891.0
26	17993.0
27	21115.0
28	24237.0
29	28831.5
30	33426.0
31	39810.0
32	46194.0
33	52039.5
34	57885.0
35	63856.5
36	69828.0
37	77510.0
38	85192.0
39	97233.0
40	109274.0
41	122451.5
42	135629.0
43	141867.5
44	148106.0
45	150273.0
46	152440.0
47	149604.5
48	146769.0
49	137130.0
50	127491.0
51	112669.0
52	97847.0
53	87430.0
54	77013.0
55	68398.5
56	59784.0
57	53328.0
58	46872.0
59	41293.5
60	35715.0
61	30456.5
62	25198.0
63	20035.0
64	14872.0
65	11491.0
66	8110.0
67	6114.0
68	4118.0
69	3240.5
70	2363.0
71	1950.5
72	1538.0
73	1080.5
74	623.0
75	436.5
76	250.0
77	182.5
78	115.0
79	91.5
80	68.0
81	43.0
82	18.0
83	13.0
84	8.0
85	4.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1667992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.00702264573233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.90842143324379	23.3358149587843
2	6.864298371217662	4.394115068292544
3	3.247200737869501	3.1179968265668347
4	2.2410667667967847	2.8691949902185128
5	1.6685789323712161	2.670312183729869
6	1.3797346304104043	2.6496718536388157
7	1.194916534834822	2.677200441315271
8	1.0437060789657342	2.6724739283955805
9	0.9148780357157194	2.6354269806512525
>10	8.442815163320907	49.36422446451628
>50	0.07972400122277701	1.6205245028999473
>100	0.013502008267140864	0.8219020234735213
>500	5.786528817800615E-4	0.10279130519589749
>1k	1.9288429392668718E-4	0.09550639587871076
>5k	3.8576858785337436E-4	0.9728440764426142
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8140	0.4880119329109492	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7618	0.45671681878570164	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030815495517964114	0.0
2	0.0	0.0	0.0	0.10641537849102393	0.0
3	0.0	0.0	0.0	0.1709240811706531	0.0
4	0.0	0.0	0.0	0.26031299910311323	0.0
5	0.0	0.0	0.0	0.4764411340102351	0.0
6	0.0	0.0	0.0	0.7857951357080849	0.0
7	0.0	0.0	0.0	0.9915515182327014	0.0
8	0.0016786651254922086	0.0	0.0	1.4502467637734473	0.0
9	0.0016786651254922086	0.0	0.0	1.9455129281195593	0.0
10	0.0016786651254922086	0.0	0.0	2.558285651250126	0.0
11	0.0016786651254922086	0.0	0.0	2.972436318639418	0.0
12	0.0016786651254922086	0.0	0.0	3.3608074858872223	0.0
13	0.0016786651254922086	0.0	0.0	3.553254452059722	0.0
14	0.0016786651254922086	0.0	0.0	3.646899985131823	0.0
15	0.0016786651254922086	0.0	0.0	3.7447421810176547	0.0
16	0.0016786651254922086	0.0	0.0	3.9264576808521863	0.0
17	0.0016786651254922086	0.0	0.0	4.149600237890829	0.0
18	0.0016786651254922086	0.0	0.0	4.469865562904379	0.0
19	0.0016786651254922086	0.0	0.0	4.638211694060883	0.0
20	0.0016786651254922086	0.0	0.0	4.818548290399474	0.0
21	0.0016786651254922086	0.0	0.0	5.011774636808809	0.0
22	0.0016786651254922086	0.0	0.0	5.230001103122797	0.0
23	0.0016786651254922086	0.0	0.0	5.4622564136998255	0.0
24	0.0016786651254922086	0.0	0.0	5.6457105309857605	0.0
25	0.0016786651254922086	0.0	0.0	5.803085386500655	0.0
26	0.0016786651254922086	0.0	0.0	5.9559638175722664	0.0
27	0.0016786651254922086	0.0	0.0	6.117355478923161	0.0
28	0.0016786651254922086	0.0	0.0	6.286900656597873	0.0
29	0.0016786651254922086	0.0	0.0	6.474191722742075	0.0
30	0.0016786651254922086	0.0	0.0	6.711602933347402	0.0
31	0.0016786651254922086	0.0	0.0	6.909505561177752	0.0
32	0.0016786651254922086	0.0	0.0	7.099674338965655	0.0
33	0.0016786651254922086	0.0	0.0	7.294699255152303	0.0
34	0.0016786651254922086	0.0	0.0	7.49505992834498	0.0
35	0.0016786651254922086	0.0	0.0	7.726535858685174	0.0
36	0.0016786651254922086	0.0	0.0	7.934870191223939	0.0
37	0.0016786651254922086	0.0	0.0	8.148240519139181	0.0
38	0.0016786651254922086	0.0	0.0	8.380076163434836	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCGA	35	1.4466059E-7	44.0	2
ACGTCGA	20	7.857506E-4	44.0	2
CCACGAC	20	7.857506E-4	44.0	18
GTTGCGA	50	2.7284841E-11	44.0	2
CGTTTTT	11365	0.0	43.07083	1
GAGCGAT	1055	0.0	42.123222	7
TATCGCG	70	0.0	40.857143	1
TATAGCG	125	0.0	40.48	1
TGCGCGA	60	3.6379788E-12	40.333332	2
TATTGCG	100	0.0	39.600002	1
ACGAGAT	345	0.0	39.536232	5
CGGTCTA	240	0.0	39.416664	31
TATGCGA	90	0.0	39.11111	2
GTTTTTA	6820	0.0	38.64516	2
CGACGGT	245	0.0	38.612244	28
TTTCGCG	40	4.1260319E-7	38.5	1
AGCGATA	240	0.0	38.5	8
GCGATGT	290	0.0	37.931038	9
TCACGAC	250	0.0	37.84	25
CGTATTG	35	7.292183E-6	37.714287	44
>>END_MODULE