FastQCFastQC Report
Thu 26 May 2016
SRR1546458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546458_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1197094
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86320.721079547637863No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80740.6744666667780475No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA408.312782E-944.02
ATTCGCG207.856613E-444.014
TCGGACG254.4429507E-544.01
CTTGCGA254.4429507E-544.02
CGGTCCG254.4429507E-544.029
TCGACGA207.856613E-444.02
TCGCGGA254.4429507E-544.02
AATCGCG207.856613E-444.01
CGGACGG207.856613E-444.02
CGACGGT650.043.99999628
CGTTTTT114450.043.442551
GCGCGAC950.041.684219
GAGCGAT6350.040.535437
GTTTTTA61100.040.2913252
CTAAACG557.8216544E-1140.0000041
TGTAACG557.8216544E-1140.0000041
TTAGCGA1650.039.9999962
GCGAGGT2050.039.7073175
GCGATAC501.3478711E-939.6000029
CGAGATT3000.038.8666656
Produced by FastQC (version 0.11.5)