##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546458_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1197094
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.666540806319304	31.0	31.0	34.0	30.0	34.0
2	32.038144038813996	33.0	31.0	34.0	30.0	34.0
3	32.08450965421262	33.0	31.0	34.0	30.0	34.0
4	35.783537466564866	37.0	35.0	37.0	35.0	37.0
5	35.739403087810985	37.0	35.0	37.0	35.0	37.0
6	35.783430540960026	37.0	35.0	37.0	35.0	37.0
7	36.1404451112444	37.0	35.0	37.0	35.0	37.0
8	18.3458491981415	28.0	0.0	37.0	0.0	37.0
9	27.91662058284479	27.0	17.0	39.0	17.0	39.0
10	33.75602083044439	32.0	32.0	39.0	27.0	39.0
11	35.914421925095276	37.0	35.0	39.0	32.0	39.0
12	36.71721686016303	38.0	35.0	39.0	33.0	39.0
13	36.93440698892485	39.0	37.0	39.0	33.0	39.0
14	38.152070764701854	40.0	37.0	41.0	33.0	41.0
15	38.37054483607803	40.0	37.0	41.0	34.0	41.0
16	38.42436266492022	40.0	37.0	41.0	34.0	41.0
17	38.38607243875585	40.0	37.0	41.0	34.0	41.0
18	38.307332590423144	40.0	37.0	41.0	34.0	41.0
19	38.27805001111024	40.0	37.0	41.0	34.0	41.0
20	38.14504875974652	40.0	36.0	41.0	34.0	41.0
21	38.04849577393254	40.0	36.0	41.0	34.0	41.0
22	38.04219718752245	40.0	36.0	41.0	34.0	41.0
23	37.87120476754541	39.0	36.0	41.0	33.0	41.0
24	37.67242171458549	39.0	35.0	41.0	33.0	41.0
25	37.554089319635715	39.0	35.0	41.0	33.0	41.0
26	37.513396608787616	39.0	35.0	41.0	33.0	41.0
27	37.36726606264838	39.0	35.0	41.0	33.0	41.0
28	37.34656426312387	39.0	35.0	41.0	33.0	41.0
29	37.24695053187135	39.0	35.0	41.0	33.0	41.0
30	37.112161618051715	39.0	35.0	41.0	32.0	41.0
31	37.01268822665555	39.0	35.0	41.0	32.0	41.0
32	36.66848384504475	39.0	35.0	41.0	31.0	41.0
33	36.392893958202116	39.0	35.0	41.0	30.0	41.0
34	36.131321349868934	39.0	35.0	41.0	29.0	41.0
35	35.88128751793928	39.0	35.0	41.0	26.0	41.0
36	35.72584358454724	39.0	35.0	41.0	25.0	41.0
37	35.653820836124815	39.0	35.0	41.0	25.0	41.0
38	35.58736657271693	39.0	35.0	41.0	24.0	41.0
39	35.50731354429978	39.0	35.0	41.0	24.0	41.0
40	35.45789637238178	39.0	35.0	41.0	24.0	41.0
41	35.44466015200143	39.0	35.0	41.0	23.0	41.0
42	35.29732752816404	39.0	35.0	41.0	23.0	41.0
43	35.30024542767736	39.0	35.0	41.0	23.0	41.0
44	35.084586506991094	39.0	35.0	40.0	23.0	41.0
45	35.013241232518084	39.0	35.0	40.0	23.0	41.0
46	35.006287726778346	39.0	35.0	40.0	23.0	41.0
47	34.901447171233	38.0	35.0	40.0	22.0	41.0
48	34.88262492335606	38.0	34.0	40.0	22.0	41.0
49	34.84776550546574	38.0	34.0	40.0	22.0	41.0
50	34.26331098476811	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	7.0
15	19.0
16	34.0
17	103.0
18	274.0
19	690.0
20	1456.0
21	2564.0
22	4202.0
23	6724.0
24	10667.0
25	16677.0
26	22421.0
27	24668.0
28	23454.0
29	22600.0
30	24605.0
31	30902.0
32	39259.0
33	52356.0
34	77794.0
35	116406.0
36	116833.0
37	184296.0
38	214794.0
39	203283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.48357606002536	22.643752286787837	26.720959256332417	19.151712396854382
2	36.50757584617415	23.75928707352973	27.87776064369214	11.855376436603976
3	26.76798981533614	23.796961642109977	37.20543248901089	12.229616053542996
4	23.984916806867297	26.39475262594249	36.2180413568191	13.402289210371116
5	21.886000598115103	30.245995719634383	34.628191269858505	13.239812412392011
6	18.91806324315384	38.43382391023595	32.26012326517383	10.38798958143638
7	82.6711185587765	2.8905833627100295	12.794734582246676	1.6435634962667929
8	42.485803119888665	50.42678352744229	6.031690076134373	1.0557232765346747
9	78.49124630146004	4.960846850790331	13.717886815905853	2.8300200318437816
10	35.6582691083574	35.343841001625606	18.963088947066815	10.034800942950179
11	28.663580303635307	22.77991536170092	34.626437021654105	13.930067313009673
12	27.339289980569614	22.4219651923742	33.3220281782383	16.916716648817886
13	21.023495230950953	28.21958843666412	34.67714314832419	16.079773184060734
14	16.7686915146179	31.23497402877301	35.20091154078126	16.795422915827828
15	16.46428768333982	25.322739901795515	42.505099850137086	15.707872564727582
16	18.927669840463658	24.24997535698951	40.36266157878997	16.45969322375686
17	19.11103054563802	23.90497321012385	34.35987483021383	22.624121414024295
18	20.170763532354187	24.736403323381456	36.70171264746127	18.391120496803094
19	22.77139472756525	25.982170155392975	32.73051239083982	18.515922726201953
20	24.533077602928426	25.48421427222925	32.20590864209494	17.77679948274739
21	21.717008021091075	26.864055788434328	33.75123423891524	17.66770195155936
22	20.612416401719496	23.288229662833494	34.695938664799925	21.403415270647084
23	18.262642699737867	27.364100062317583	34.22237518524025	20.1508820527043
24	18.661775934053633	24.6996476467178	38.69462214329034	17.943954275938232
25	17.93977749449918	27.166788907136784	35.916978950692254	18.97645464767178
26	18.171338257480198	30.198296875600413	33.174253650924655	18.456111215994735
27	18.694271293649454	27.782446491252983	34.660436022567986	18.862846192529574
28	17.15805108036629	26.805831455173944	38.35688759612862	17.67922986833114
29	18.09849518918314	24.401676058855863	37.953911722888925	19.54591702907207
30	19.84881721903209	26.401769618760095	35.30909018005269	18.44032298215512
31	23.93930635355285	25.362335789837726	31.69341755952331	19.004940297086108
32	24.16735862012507	26.43200951637883	32.086369157309285	17.314262706186817
33	23.852763442135704	26.605763624243377	31.275154666216686	18.26631826740423
34	20.781576050001085	27.57018245852038	32.040341025850935	19.607900465627594
35	21.70046796659243	27.088599558597735	31.96708027940997	19.24385219539986
36	24.354896106738487	27.8876178478883	29.65640125169786	18.10108479367535
37	21.915906353218713	29.907676423071205	30.412231620908635	17.76418560280145
38	21.282205073285805	30.494931893401855	28.92162186093991	19.30124117237243
39	21.002110109983008	29.98118777639851	29.33403726023186	19.682664853386616
40	22.266588922841482	27.441955268341502	29.59617206334674	20.69528374547028
41	19.39864371553111	26.66532452756425	30.64170399317013	23.29432776373451
42	21.19014881036911	28.23880163128376	28.89330328278314	21.67774627556399
43	21.43048081437214	27.247233717652918	29.932570040447953	21.389715427526994
44	20.75451050627603	29.064050108011568	30.19428716541892	19.987152220293478
45	19.806548190868885	29.77786205594548	29.14424431164136	21.271345441544273
46	21.477845515890984	29.146666844876005	29.414899748891898	19.96058789034111
47	21.283625178975086	26.95377305374515	30.27247651395797	21.490125253321793
48	21.47107912995972	25.617787742650115	31.526513373218812	21.384619754171354
49	20.907130100058975	25.44461838418704	31.97869173181053	21.66955978394345
50	20.124735400895837	27.9360685125813	31.267302317111273	20.671893769411593
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	388.0
1	723.5
2	1059.0
3	14237.0
4	27415.0
5	19353.0
6	11291.0
7	11221.0
8	11151.0
9	11387.5
10	11624.0
11	11633.5
12	11643.0
13	11289.0
14	10935.0
15	10425.5
16	9916.0
17	9308.5
18	8701.0
19	8473.0
20	8245.0
21	8259.5
22	8274.0
23	9082.0
24	9890.0
25	11499.0
26	13108.0
27	15823.5
28	18539.0
29	21424.5
30	24310.0
31	27604.0
32	30898.0
33	34749.0
34	38600.0
35	43072.5
36	47545.0
37	52408.0
38	57271.0
39	64831.5
40	72392.0
41	81201.5
42	90011.0
43	95290.0
44	100569.0
45	104031.0
46	107493.0
47	106471.5
48	105450.0
49	99017.5
50	92585.0
51	81943.5
52	71302.0
53	63054.0
54	54806.0
55	48690.0
56	42574.0
57	37914.5
58	33255.0
59	29436.0
60	25617.0
61	21512.5
62	17408.0
63	14031.5
64	10655.0
65	8087.0
66	5519.0
67	4261.0
68	3003.0
69	2364.5
70	1726.0
71	1349.5
72	973.0
73	749.0
74	525.0
75	390.0
76	255.0
77	163.5
78	72.0
79	59.5
80	47.0
81	40.5
82	34.0
83	21.5
84	9.0
85	6.0
86	3.0
87	3.5
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1197094.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.84066767395185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.31423197849695	26.228968173735094
2	8.399627841239706	6.356950514504506
3	4.533241871076975	5.146226973872025
4	3.1983880055707807	4.841165504446303
5	2.436919278567812	4.610732628426554
6	2.0141039941066996	4.572902394106242
7	1.645049080424657	4.357482889178268
8	1.3960829018017107	4.226296730589191
9	1.1954207734619373	4.07119681972005
>10	5.8323100745158385	32.48893900963504
>50	0.02308808673959289	0.5624549381097833
>100	0.009983175838151524	0.8584613407450222
>500	0.0011092415422798863	0.27577207455511626
>1k	0.0	0.0
>5k	4.4369661691195445E-4	1.4024500083767952
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8632	0.721079547637863	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8074	0.6744666667780475	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03291303773972637	0.0
2	0.0	0.0	0.0	0.11110238627877175	0.0
3	0.0	0.0	0.0	0.20691775249061478	0.0
4	0.0	0.0	0.0	0.31409396421667807	0.0
5	0.0	0.0	0.0	0.5682093469685756	0.0
6	0.0	0.0	0.0	0.9929044836913392	0.0
7	0.0	0.0	0.0	1.2733335895092617	0.0
8	0.0	0.0	0.0	1.809047576882016	0.0
9	0.0	0.0	0.0	2.4035706469166165	0.0
10	0.0	0.0	0.0	3.057069870870625	0.0
11	0.0	0.0	0.0	3.4380758737409094	0.0
12	0.0	0.0	0.0	3.806551532294039	0.0
13	0.0	0.0	0.0	4.0230758820944725	0.0
14	0.0	0.0	0.0	4.13710201538058	0.0
15	0.0	0.0	0.0	4.243526406447614	0.0
16	0.0	0.0	0.0	4.40032278166961	0.0
17	0.0	0.0	0.0	4.582430452412258	0.0
18	0.0	0.0	0.0	4.849159715110091	0.0
19	0.0	0.0	0.0	5.001528702006693	0.0
20	0.0	0.0	0.0	5.159327504774061	0.0
21	0.0	0.0	0.0	5.324811585389284	0.0
22	0.0	0.0	0.0	5.518864851047621	0.0
23	0.0	0.0	0.0	5.716927826887446	0.0
24	0.0	0.0	0.0	5.875812592828967	0.0
25	0.0	0.0	0.0	6.020830444392838	0.0
26	0.0	0.0	0.0	6.155072199843955	0.0
27	0.0	0.0	0.0	6.307858864884462	0.0
28	0.0	0.0	0.0	6.460561994296187	0.0
29	0.0	0.0	0.0	6.627549716229469	0.0
30	0.0	0.0	0.0	6.845995385491866	0.0
31	0.0	0.0	0.0	7.021169599045689	0.0
32	0.0	0.0	0.0	7.193169458705833	0.0
33	0.0	0.0	0.0	7.361243143813268	0.0
34	0.0	0.0	0.0	7.540092925033456	0.0
35	0.0	0.0	0.0	7.741497326024523	0.0
36	0.0	0.0	0.0	7.9289512770091575	0.0
37	0.0	0.0	0.0	8.120999687576749	0.0
38	0.0	0.0	0.0	8.3259961206054	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCGA	40	8.312782E-9	44.0	2
ATTCGCG	20	7.856613E-4	44.0	14
TCGGACG	25	4.4429507E-5	44.0	1
CTTGCGA	25	4.4429507E-5	44.0	2
CGGTCCG	25	4.4429507E-5	44.0	29
TCGACGA	20	7.856613E-4	44.0	2
TCGCGGA	25	4.4429507E-5	44.0	2
AATCGCG	20	7.856613E-4	44.0	1
CGGACGG	20	7.856613E-4	44.0	2
CGACGGT	65	0.0	43.999996	28
CGTTTTT	11445	0.0	43.44255	1
GCGCGAC	95	0.0	41.68421	9
GAGCGAT	635	0.0	40.53543	7
GTTTTTA	6110	0.0	40.291325	2
CTAAACG	55	7.8216544E-11	40.000004	1
TGTAACG	55	7.8216544E-11	40.000004	1
TTAGCGA	165	0.0	39.999996	2
GCGAGGT	205	0.0	39.707317	5
GCGATAC	50	1.3478711E-9	39.600002	9
CGAGATT	300	0.0	38.866665	6
>>END_MODULE