FastQCFastQC Report
Thu 26 May 2016
SRR1546457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546457_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1570342
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78040.49696180831946163No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71380.45455066475965106No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17140.10914819829056345No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGACA207.857363E-444.00000424
CTTCGCG254.4435867E-544.01
GCGTTAG1700.044.01
TCTAGCG302.5283116E-644.01
CTATGCG351.4465331E-743.9999961
CGTTTTT107250.043.1384621
CGACGGT3800.042.84210628
GAGCGAT9850.041.7664957
CGTTAGA1950.041.743592
CGGTCTA3900.041.7435931
TAGTGCG700.040.857141
AGTTGCG650.040.6153831
ACGTCGC1250.040.4820
AAGCGAC1200.040.33333215
TCACGAC4050.040.1975325
ACGGTCT4050.040.1975330
GTGATCG557.8216544E-1140.01
TACGTAG501.3478711E-939.6000021
GTTTTTA62200.038.8006442
GACCGAT5700.038.596499
Produced by FastQC (version 0.11.5)