##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546457_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1570342
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.546964291854895	31.0	31.0	34.0	30.0	34.0
2	31.92057972085062	31.0	31.0	34.0	30.0	34.0
3	32.00855418755914	33.0	31.0	34.0	30.0	34.0
4	35.70339645758695	37.0	35.0	37.0	35.0	37.0
5	35.644901556476235	37.0	35.0	37.0	33.0	37.0
6	35.692346635318934	37.0	35.0	37.0	33.0	37.0
7	36.07460222040804	37.0	35.0	37.0	35.0	37.0
8	18.35576645087503	29.0	0.0	37.0	0.0	37.0
9	27.903592338484227	27.0	17.0	39.0	17.0	39.0
10	33.60947551552464	32.0	32.0	39.0	27.0	39.0
11	35.73260410789497	37.0	35.0	39.0	32.0	39.0
12	36.486442443747926	37.0	35.0	39.0	32.0	39.0
13	36.68274808926972	39.0	35.0	39.0	33.0	39.0
14	37.8667990794362	40.0	36.0	41.0	33.0	41.0
15	38.11716301289783	40.0	37.0	41.0	33.0	41.0
16	38.191032908754906	40.0	37.0	41.0	33.0	41.0
17	38.16433044521512	40.0	37.0	41.0	33.0	41.0
18	38.11038487157575	40.0	37.0	41.0	33.0	41.0
19	38.110692447887146	40.0	37.0	41.0	34.0	41.0
20	37.99758969702141	40.0	36.0	41.0	34.0	41.0
21	37.87188013821193	40.0	36.0	41.0	33.0	41.0
22	37.877161790234226	40.0	36.0	41.0	33.0	41.0
23	37.727234576926556	39.0	36.0	41.0	33.0	41.0
24	37.57437551819922	39.0	35.0	41.0	33.0	41.0
25	37.45851922702188	39.0	35.0	41.0	33.0	41.0
26	37.40622807006372	39.0	35.0	41.0	33.0	41.0
27	37.24781034959263	39.0	35.0	41.0	32.0	41.0
28	37.25586146202547	39.0	35.0	41.0	33.0	41.0
29	37.17565154596897	39.0	35.0	41.0	32.0	41.0
30	37.073437505970034	39.0	35.0	41.0	32.0	41.0
31	37.0241667101816	39.0	35.0	41.0	32.0	41.0
32	36.73481827525469	39.0	35.0	41.0	31.0	41.0
33	36.556788266505	39.0	35.0	41.0	30.0	41.0
34	36.402040447240154	39.0	35.0	41.0	30.0	41.0
35	36.208988869940434	39.0	35.0	41.0	30.0	41.0
36	36.06881430923964	39.0	35.0	41.0	29.0	41.0
37	36.002119283570075	39.0	35.0	41.0	29.0	41.0
38	35.94127967028838	39.0	35.0	41.0	28.0	41.0
39	35.86910876738953	39.0	35.0	41.0	27.0	41.0
40	35.81699145791172	39.0	35.0	41.0	27.0	41.0
41	35.84260498668443	39.0	35.0	41.0	27.0	41.0
42	35.68325498521978	39.0	35.0	41.0	27.0	41.0
43	35.709369678706935	39.0	35.0	41.0	27.0	41.0
44	35.48292346507958	39.0	35.0	40.0	26.0	41.0
45	35.412449007923115	38.0	35.0	40.0	25.0	41.0
46	35.41393721877145	38.0	35.0	40.0	26.0	41.0
47	35.29181286624188	38.0	35.0	40.0	25.0	41.0
48	35.28642996239036	38.0	35.0	40.0	25.0	41.0
49	35.25283345920825	38.0	35.0	40.0	26.0	41.0
50	34.64376295100048	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	3.0
14	8.0
15	15.0
16	52.0
17	164.0
18	396.0
19	947.0
20	1916.0
21	3304.0
22	5133.0
23	7917.0
24	12255.0
25	18161.0
26	24634.0
27	26989.0
28	27099.0
29	28750.0
30	33702.0
31	42483.0
32	55802.0
33	73849.0
34	109279.0
35	167714.0
36	155601.0
37	243343.0
38	278038.0
39	252786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.43824720984346	24.112772886415826	28.634208344424334	15.814771559316378
2	32.842399935810164	24.71359742017981	29.48446898828408	12.959533655725949
3	27.500378898354626	24.805806633204742	34.51362824149135	13.180186226949289
4	24.732128415338824	26.94508584754149	34.40040449787371	13.922381239245974
5	22.092384970917163	31.51721090055542	33.17742249777437	13.212981630753045
6	19.072342203163387	40.08528078596892	30.110765680342244	10.731611330525451
7	85.20073971147686	3.1703285016894407	9.989352637833033	1.6395791490006635
8	43.73505898715057	50.4339818969371	4.6281001208653905	1.2028589950469388
9	81.43996658052832	4.650961382934418	11.140503151542786	2.7685688849944787
10	41.02329301515211	32.062697170425295	16.915423519207916	9.998586295214674
11	31.67150849942242	22.80388603246936	31.077879850376544	14.446725617731678
12	29.47638157802568	22.539867111750176	30.838887325181393	17.14486398504275
13	20.84195672025584	31.445315733770094	31.588596624174865	16.1241309217992
14	16.305174286875086	34.0369804794115	32.4246565397856	17.23318869392782
15	15.589470319204352	25.039832087532528	43.13041362964246	16.240283963620662
16	17.403087989750006	23.593522939588958	40.84416006194829	18.159229008712753
17	18.394082308185094	23.510037940779778	32.18400832430133	25.911871426733796
18	20.088999721079865	24.979463072375317	35.385285498318204	19.546251708226617
19	23.5397130051925	26.736532551507885	30.37261946760642	19.351134975693192
20	25.18846213117907	25.826157614073875	29.769693480783165	19.21568677396389
21	21.093112201036462	27.59621789393648	32.067091117731046	19.243578787296016
22	20.734145810275724	24.15754020461785	31.624321326182447	23.48399265892398
23	18.386822743071253	28.765198918452157	31.329162691948632	21.51881564652795
24	18.01868637532461	25.274048583047517	37.97236525546664	18.73489978616123
25	17.611577605387872	27.649136302792638	34.86075007864529	19.8785360131742
26	17.817583685592055	30.96159944776361	31.302225884552538	19.9185909820918
27	18.856784063598887	29.522931947308294	32.31264272368694	19.30764126540588
28	16.853717215740264	27.264634073341988	37.28219712648582	18.599451584431925
29	17.613997460425818	24.57655720855712	36.2742638227851	21.535181508231965
30	19.926359990371527	27.522221274091883	32.742612755692704	19.80880597984388
31	24.673733492449415	25.874936797207233	28.640512703602145	20.810817006741207
32	24.767279993784793	27.14166722917683	28.820537182346268	19.27051559469211
33	23.83060505291204	27.67683727493756	28.501689440898865	19.990868231251536
34	20.15923919757607	27.21165198409009	30.311549968096124	22.317558850237717
35	19.82115997661656	27.82030920652953	30.73005752886951	21.628473287984402
36	24.02553074425826	28.170360341887307	28.526397434444217	19.27771147941022
37	19.935020524191547	31.198235798316542	29.19510527006219	19.67163840742972
38	19.641835982225526	31.980485779530827	28.075349191450016	20.30232904679363
39	19.967306484829418	31.112394624865157	28.43144996440266	20.488848925902765
40	21.309880268119937	27.659516207297518	28.734887050082087	22.29571647450046
41	18.332630726300387	26.904712476645216	28.90994445795884	25.85271233909556
42	20.703897622301383	27.987470245335093	27.47318736937559	23.835444762987933
43	20.785790611217177	27.768473364400876	28.714445643051008	22.731290381330947
44	20.134785925613656	29.44976317260826	29.33959608798593	21.075854813792155
45	18.77590996101486	31.37736875152037	27.63780119235173	22.208920095113037
46	21.359933059168004	29.996968813162994	28.394706376063304	20.248391751605702
47	20.674349918680136	26.938463086385006	29.16810478227036	23.219082212664503
48	21.17767976657314	25.327285393882352	30.886711302378718	22.608323537165788
49	20.208527823875308	25.192091913735986	31.74760657232628	22.85177369006242
50	19.023690380821503	29.216119800654887	30.02925477380087	21.73093504472274
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	392.0
1	816.0
2	1240.0
3	12695.5
4	24151.0
5	17145.5
6	10140.0
7	10176.0
8	10212.0
9	10558.0
10	10904.0
11	10994.0
12	11084.0
13	10643.5
14	10203.0
15	9840.5
16	9478.0
17	9160.5
18	8843.0
19	8762.5
20	8682.0
21	9270.0
22	9858.0
23	11183.0
24	12508.0
25	14323.5
26	16139.0
27	19031.0
28	21923.0
29	25852.0
30	29781.0
31	35269.5
32	40758.0
33	45734.5
34	50711.0
35	56374.0
36	62037.0
37	68606.0
38	75175.0
39	86924.0
40	98673.0
41	113376.5
42	128080.0
43	135966.5
44	143853.0
45	147800.0
46	151747.0
47	149163.0
48	146579.0
49	137065.0
50	127551.0
51	112340.5
52	97130.0
53	84718.0
54	72306.0
55	63035.5
56	53765.0
57	47836.0
58	41907.0
59	37340.5
60	32774.0
61	27411.0
62	22048.0
63	17548.0
64	13048.0
65	10009.0
66	6970.0
67	5417.5
68	3865.0
69	3182.5
70	2500.0
71	2050.0
72	1600.0
73	1228.5
74	857.0
75	664.0
76	471.0
77	346.5
78	222.0
79	168.0
80	114.0
81	81.5
82	49.0
83	27.0
84	5.0
85	4.0
86	3.0
87	2.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1570342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.94094275324756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.92623009846363	24.35199713636898
2	6.946254123961679	4.576323188938629
3	3.163793339472924	3.1265500583605057
4	2.096321195356697	2.7621918595465784
5	1.642006790925023	2.7044625850152464
6	1.3172792041064811	2.6035451311509066
7	1.1213445239601751	2.5856702039307837
8	0.9470480366928112	2.495732412901872
9	0.839288954907284	2.488227247533044
>10	7.887250161778901	47.95300355272895
>50	0.094089811239532	1.9753285774833744
>100	0.017125337694015426	1.016136169661673
>500	0.0013778950085848699	0.2808292207842631
>1k	1.9684214408355284E-4	0.1111386018065636
>5k	3.936842881671057E-4	0.9688640537886074
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7804	0.49696180831946163	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7138	0.45455066475965106	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1714	0.10914819829056345	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.040628092479217906	0.0
2	0.0	0.0	0.0	0.13621236647813023	0.0
3	0.0	0.0	0.0	0.21613126312612158	0.0
4	0.0	0.0	0.0	0.3398622720401034	0.0
5	0.0	0.0	0.0	0.6367402769587771	0.0
6	0.0	0.0	0.0	1.0467783450993478	0.0
7	0.0	0.0	0.0	1.340217608648307	0.0
8	0.0	0.0	0.0	1.993578468894037	0.0
9	0.0	0.0	0.0	2.603509299248189	0.0
10	0.0	0.0	0.0	3.379900684054811	0.0
11	0.0	0.0	0.0	3.872404864672791	0.0
12	0.0	0.0	0.0	4.389489678044655	0.0
13	0.0	0.0	0.0	4.624024575538322	0.0
14	0.0	0.0	0.0	4.738967689840812	0.0
15	0.0	0.0	0.0	4.856903782742868	0.0
16	0.0	0.0	0.0	5.069277902520597	0.0
17	0.0	0.0	0.0	5.335334595903313	0.0
18	0.0	0.0	0.0	5.703343602858485	0.0
19	0.0	0.0	0.0	5.90081651003412	0.0
20	6.368039573545126E-5	0.0	0.0	6.111853341501406	0.0
21	6.368039573545126E-5	0.0	0.0	6.357723349435982	0.0
22	6.368039573545126E-5	0.0	0.0	6.618558250368391	0.0
23	6.368039573545126E-5	0.0	0.0	6.902954897722917	0.0
24	6.368039573545126E-5	0.0	0.0	7.122270180635811	0.0
25	6.368039573545126E-5	0.0	0.0	7.3061791635197935	0.0
26	6.368039573545126E-5	0.0	0.0	7.481618653770962	0.0
27	6.368039573545126E-5	0.0	0.0	7.670813109500988	0.0
28	6.368039573545126E-5	0.0	0.0	7.873507809126929	0.0
29	6.368039573545126E-5	0.0	0.0	8.091676844916584	0.0
30	6.368039573545126E-5	0.0	0.0	8.371743225361099	0.0
31	6.368039573545126E-5	0.0	0.0	8.607424369978006	0.0
32	6.368039573545126E-5	0.0	0.0	8.821135778066179	0.0
33	6.368039573545126E-5	0.0	0.0	9.037203360796566	0.0
34	6.368039573545126E-5	0.0	0.0	9.262313559721386	0.0
35	6.368039573545126E-5	0.0	0.0	9.521110687990259	0.0
36	6.368039573545126E-5	0.0	0.0	9.759020646457905	0.0
37	6.368039573545126E-5	0.0	0.0	10.0043812112266	0.0
38	6.368039573545126E-5	0.0	0.0	10.253180517365006	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGACA	20	7.857363E-4	44.000004	24
CTTCGCG	25	4.4435867E-5	44.0	1
GCGTTAG	170	0.0	44.0	1
TCTAGCG	30	2.5283116E-6	44.0	1
CTATGCG	35	1.4465331E-7	43.999996	1
CGTTTTT	10725	0.0	43.138462	1
CGACGGT	380	0.0	42.842106	28
GAGCGAT	985	0.0	41.766495	7
CGTTAGA	195	0.0	41.74359	2
CGGTCTA	390	0.0	41.74359	31
TAGTGCG	70	0.0	40.85714	1
AGTTGCG	65	0.0	40.615383	1
ACGTCGC	125	0.0	40.48	20
AAGCGAC	120	0.0	40.333332	15
TCACGAC	405	0.0	40.19753	25
ACGGTCT	405	0.0	40.19753	30
GTGATCG	55	7.8216544E-11	40.0	1
TACGTAG	50	1.3478711E-9	39.600002	1
GTTTTTA	6220	0.0	38.800644	2
GACCGAT	570	0.0	38.59649	9
>>END_MODULE