FastQCFastQC Report
Thu 26 May 2016
SRR1546456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546456_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1221691
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63860.5227181013857023No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57920.4740969688734713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA302.5278478E-644.0000042
GGTCGTT207.8566774E-444.09
CGTCGGT207.8566774E-444.016
TGTAACG351.4461875E-744.01
TAATGCG351.4461875E-744.01
TTCTCGC207.8566774E-444.014
CGTTTTT81650.043.3533361
CGACGGT1150.042.0869628
CGAGATT4050.040.1975336
TGCGTAG1650.040.0000041
GCGAGAC1800.039.111115
GACGGTC1250.038.7229
TACGCGA404.1248313E-738.52
GAGCGAT6600.038.3333367
ACGAGAT3100.038.322585
GTTTTTA50450.038.156592
TGTTCGG357.290586E-637.7142872
GCTAGCG700.037.7142871
TCACGAC1350.037.4814825
TGCGCGA651.0913936E-1137.2307662
Produced by FastQC (version 0.11.5)