##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546456_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1221691
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57306307405064	31.0	31.0	34.0	30.0	34.0
2	31.945784163098526	31.0	31.0	34.0	30.0	34.0
3	32.02472965749932	33.0	31.0	34.0	30.0	34.0
4	35.71428700055906	37.0	35.0	37.0	35.0	37.0
5	35.66168777538674	37.0	35.0	37.0	33.0	37.0
6	35.7110357692739	37.0	35.0	37.0	33.0	37.0
7	36.09002603768056	37.0	35.0	37.0	35.0	37.0
8	18.313846954753696	28.0	0.0	37.0	0.0	37.0
9	27.882814885269678	27.0	17.0	39.0	17.0	39.0
10	33.65969136221843	32.0	32.0	39.0	27.0	39.0
11	35.79017280146944	37.0	35.0	39.0	32.0	39.0
12	36.56492926607464	37.0	35.0	39.0	33.0	39.0
13	36.76141675759255	39.0	35.0	39.0	33.0	39.0
14	37.94384341048596	40.0	37.0	41.0	33.0	41.0
15	38.17834215034735	40.0	37.0	41.0	33.0	41.0
16	38.25236004849017	40.0	37.0	41.0	33.0	41.0
17	38.218483233485394	40.0	37.0	41.0	33.0	41.0
18	38.16157277085613	40.0	37.0	41.0	33.0	41.0
19	38.16346359267605	40.0	37.0	41.0	34.0	41.0
20	38.03564321911187	40.0	36.0	41.0	34.0	41.0
21	37.922542606927614	40.0	36.0	41.0	33.0	41.0
22	37.92292404544193	40.0	36.0	41.0	33.0	41.0
23	37.77057701169936	39.0	36.0	41.0	33.0	41.0
24	37.61775031493234	39.0	35.0	41.0	33.0	41.0
25	37.50691214063131	39.0	35.0	41.0	33.0	41.0
26	37.4416558687917	39.0	35.0	41.0	33.0	41.0
27	37.28522924372857	39.0	35.0	41.0	32.0	41.0
28	37.274259203022694	39.0	35.0	41.0	33.0	41.0
29	37.1912259319255	39.0	35.0	41.0	32.0	41.0
30	37.08885143624697	39.0	35.0	41.0	32.0	41.0
31	37.04154323802009	39.0	35.0	41.0	32.0	41.0
32	36.766849391540084	39.0	35.0	41.0	31.0	41.0
33	36.59419362179143	39.0	35.0	41.0	30.0	41.0
34	36.4382703973427	39.0	35.0	41.0	30.0	41.0
35	36.236327352824894	39.0	35.0	41.0	30.0	41.0
36	36.09433481952474	39.0	35.0	41.0	29.0	41.0
37	36.03467816329989	39.0	35.0	41.0	28.0	41.0
38	35.957726626454644	39.0	35.0	41.0	28.0	41.0
39	35.882300025129105	39.0	35.0	41.0	27.0	41.0
40	35.82869727287833	39.0	35.0	41.0	27.0	41.0
41	35.83324834184749	39.0	35.0	41.0	27.0	41.0
42	35.674436498263475	39.0	35.0	41.0	26.0	41.0
43	35.69983162681889	39.0	35.0	41.0	27.0	41.0
44	35.47634139892985	39.0	35.0	40.0	25.0	41.0
45	35.39707994902148	39.0	35.0	40.0	25.0	41.0
46	35.401086690497024	39.0	35.0	40.0	25.0	41.0
47	35.29890618822599	38.0	35.0	40.0	24.0	41.0
48	35.30526376964388	38.0	35.0	40.0	25.0	41.0
49	35.268937890186635	38.0	35.0	40.0	25.0	41.0
50	34.660254516076485	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	3.0
14	9.0
15	20.0
16	41.0
17	115.0
18	321.0
19	753.0
20	1472.0
21	2497.0
22	4036.0
23	6278.0
24	9479.0
25	14270.0
26	18985.0
27	21361.0
28	21451.0
29	22303.0
30	26072.0
31	32845.0
32	42359.0
33	55913.0
34	83054.0
35	126336.0
36	121314.0
37	190789.0
38	218889.0
39	200724.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.786106306750234	23.100276583849762	28.62303151942676	16.490585589973243
2	33.90521825895419	24.462896100568802	29.211560042596695	12.420325597880314
3	27.30232112702803	24.84367978482284	35.1913045115336	12.662694576615527
4	24.58788678970378	27.086636473543635	34.767383896582686	13.558092840169897
5	22.484327051603064	30.84233247195895	33.11778510277967	13.555555373658315
6	19.207311832533758	40.46023094219406	29.923032910940655	10.40942431433153
7	85.61829464242595	2.9310193821514603	9.820077253577214	1.6306087218453766
8	43.85789860120112	50.42199705162762	4.65281319089688	1.067291156274377
9	81.58552367169767	4.895673292182721	10.877136690046829	2.6416663460727796
10	39.545433337889854	34.77376848974086	16.187726683752274	9.493071488617007
11	30.64678384305033	23.13179028084843	32.258075077904316	13.963350798196924
12	29.09197170151863	22.347222006219248	31.68976443306859	16.87104185919353
13	21.268225762488225	29.653324776887118	32.63648500316364	16.44196445746101
14	16.639150161538392	33.19914773866714	32.81844590817155	17.34325619162292
15	16.145981266948844	25.581918832176058	42.50943978469187	15.762660116183225
16	18.613872083857537	23.799553242186445	40.507214999537524	17.07935967441849
17	18.79214956973572	23.503979320466467	32.76082086223112	24.943050247566692
18	20.348271371402422	25.06345712622914	35.45782034900806	19.130451153360383
19	24.002305001837616	26.605745642719803	30.305699231638773	19.08625012380381
20	24.93584711682414	25.942566491854326	30.56795867367444	18.553627717647096
21	22.21461891754953	27.00044446590832	32.43316026720341	18.351776349338746
22	21.560771095145988	24.428353814507926	31.72913609087732	22.28173899946877
23	18.395649963861565	28.613618337206383	32.00121798392556	20.989513715006495
24	18.37608691559486	25.689720232039033	37.27489193257542	18.659300919790685
25	18.217372477983385	27.29929253796582	34.716716420109506	19.76661856394129
26	18.243974949475767	31.121535641991304	31.15542309798468	19.479066310548248
27	18.890210372344562	29.020595224160612	33.06556240489616	19.023631998598663
28	17.334497839470046	27.465291960078286	36.93094243961853	18.26926776083314
29	17.984171120193242	25.268910059908766	36.392099147820524	20.354819672077472
30	20.151904204909425	26.795564508537755	33.05393917119795	19.998592115354867
31	23.568152667081936	26.022946882640536	29.616736146865286	20.79216430341224
32	24.63953651127822	26.708144694525863	29.8745754859453	18.777743308250614
33	23.01629462769227	26.79875680511684	29.788629039585295	20.396319527605588
34	19.402614900167066	27.270070746203416	31.043119741407605	22.28419461222191
35	19.651041057026696	26.87676343690835	32.27771997992946	21.194475526135495
36	23.635763871551806	27.116021972822914	29.664784303068455	19.583429852556826
37	20.494216622697557	29.028125769936914	30.914609340659787	19.56304826670574
38	19.91837543208553	30.65112209224755	28.500578296803365	20.92992417886356
39	20.62444595237257	29.2053391569554	29.897494538307967	20.27272035236406
40	21.875744357615797	27.11495787396322	29.41300214211286	21.596295626308127
41	19.12881407819162	26.263596932448547	29.94660679337083	24.660982195989
42	21.22574366185885	27.499261269830093	28.279327587745186	22.99566748056587
43	21.208063250036222	27.470530600618325	28.911811579196378	22.40959457014908
44	20.576561503686282	29.24413783845506	29.62254776371439	20.556752894144264
45	19.26845659008702	30.446815111186055	28.331959554420884	21.95276874430605
46	21.125636515289052	29.423315715676058	28.92679081699055	20.52425695204434
47	20.909706300529347	27.099323806101545	29.834467144310633	22.156502749058475
48	21.306778882712567	25.19155825818476	31.028058649854994	22.473604209247675
49	20.521310216740567	25.361159245668503	31.75475631726844	22.362774220322486
50	19.398604066003596	28.77691658529039	30.15418792476985	21.670291423936167
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	340.0
1	695.5
2	1051.0
3	10551.5
4	20052.0
5	14332.0
6	8612.0
7	8609.0
8	8606.0
9	8794.0
10	8982.0
11	8860.0
12	8738.0
13	8517.5
14	8297.0
15	7894.0
16	7491.0
17	7286.0
18	7081.0
19	7013.0
20	6945.0
21	7414.0
22	7883.0
23	8977.5
24	10072.0
25	11766.0
26	13460.0
27	15437.0
28	17414.0
29	20781.0
30	24148.0
31	27985.5
32	31823.0
33	36266.0
34	40709.0
35	45584.0
36	50459.0
37	55399.0
38	60339.0
39	69293.0
40	78247.0
41	87825.5
42	97404.0
43	102750.5
44	108097.0
45	109484.0
46	110871.0
47	109118.5
48	107366.0
49	101400.5
50	95435.0
51	85132.0
52	74829.0
53	65883.5
54	56938.0
55	50270.0
56	43602.0
57	39549.5
58	35497.0
59	31591.5
60	27686.0
61	23114.0
62	18542.0
63	14973.5
64	11405.0
65	8661.0
66	5917.0
67	4637.5
68	3358.0
69	2624.0
70	1890.0
71	1495.5
72	1101.0
73	849.0
74	597.0
75	408.5
76	220.0
77	158.5
78	97.0
79	76.5
80	56.0
81	40.5
82	25.0
83	15.0
84	5.0
85	4.0
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1221691.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.24912614497808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.73368329925835	24.225668420152285
2	7.343933298848862	5.030465959051593
3	3.7586366520138372	3.8619006248388
4	2.6654749250100305	3.651607477717781
5	2.077705098614724	3.5579792007259905
6	1.6872724666299237	3.4672564530333947
7	1.4551992674327943	3.488751229346983
8	1.2583166151554286	3.4476995586224093
9	1.114973102160249	3.4368168981729506
>10	7.862798889970972	43.332625336980946
>50	0.030728259103931843	0.681149290957763
>100	0.010558245596210658	0.7271661282071611
>500	0.0	0.0
>1k	2.3996006824909501E-4	0.09007391221354004
>5k	4.7992013649819003E-4	1.0008395099785496
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6386	0.5227181013857023	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5792	0.4740969688734713	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02995847558834435	0.0
2	0.0	0.0	0.0	0.10133495294636696	0.0
3	0.0	0.0	0.0	0.16509903076964633	0.0
4	0.0	0.0	0.0	0.2656972998900704	0.0
5	0.0	0.0	0.0	0.4831827360601003	0.0
6	0.0	0.0	0.0	0.8221391497522696	0.0
7	0.0	0.0	0.0	1.0452724952545283	0.0
8	0.0	0.0	0.0	1.5703643556349356	0.0
9	0.0	0.0	0.0	2.0964384611166	0.0
10	0.0	0.0	0.0	2.746029888081356	0.0
11	0.0	0.0	0.0	3.1417928101295662	0.0
12	0.0	0.0	0.0	3.5161919012254326	0.0
13	0.0	0.0	0.0	3.702654762947423	0.0
14	0.0	0.0	0.0	3.7986692215953135	0.0
15	0.0	0.0	0.0	3.8986126606482325	0.0
16	0.0	0.0	0.0	4.065103205311327	0.0
17	0.0	0.0	0.0	4.27383028932848	0.0
18	0.0	0.0	0.0	4.555161657080228	0.0
19	0.0	0.0	0.0	4.711829750730749	0.0
20	0.0	0.0	0.0	4.891171335468625	0.0
21	0.0	0.0	0.0	5.079189418600939	0.0
22	0.0	0.0	0.0	5.29364626570876	0.0
23	0.0	0.0	0.0	5.522509374301685	0.0
24	0.0	0.0	0.0	5.69972276132017	0.0
25	0.0	0.0	0.0	5.8500881155709585	0.0
26	0.0	0.0	0.0	6.002417960024261	0.0
27	8.185375843809933E-5	0.0	0.0	6.1639972791810695	0.0
28	8.185375843809933E-5	0.0	0.0	6.3248399145119345	0.0
29	8.185375843809933E-5	0.0	0.0	6.510402384891106	0.0
30	8.185375843809933E-5	0.0	0.0	6.743849303956565	0.0
31	8.185375843809933E-5	0.0	0.0	6.942835790719585	0.0
32	1.6370751687619866E-4	0.0	0.0	7.129626067475328	0.0
33	1.6370751687619866E-4	0.0	0.0	7.317889711882955	0.0
34	1.6370751687619866E-4	0.0	0.0	7.514666147168146	0.0
35	1.6370751687619866E-4	0.0	0.0	7.735262026158824	0.0
36	1.6370751687619866E-4	0.0	0.0	7.940223837287824	0.0
37	1.6370751687619866E-4	0.0	0.0	8.14665901606871	0.0
38	1.6370751687619866E-4	0.0	0.0	8.365208551098437	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCGA	30	2.5278478E-6	44.000004	2
GGTCGTT	20	7.8566774E-4	44.0	9
CGTCGGT	20	7.8566774E-4	44.0	16
TGTAACG	35	1.4461875E-7	44.0	1
TAATGCG	35	1.4461875E-7	44.0	1
TTCTCGC	20	7.8566774E-4	44.0	14
CGTTTTT	8165	0.0	43.353336	1
CGACGGT	115	0.0	42.08696	28
CGAGATT	405	0.0	40.197533	6
TGCGTAG	165	0.0	40.000004	1
GCGAGAC	180	0.0	39.11111	5
GACGGTC	125	0.0	38.72	29
TACGCGA	40	4.1248313E-7	38.5	2
GAGCGAT	660	0.0	38.333336	7
ACGAGAT	310	0.0	38.32258	5
GTTTTTA	5045	0.0	38.15659	2
TGTTCGG	35	7.290586E-6	37.714287	2
GCTAGCG	70	0.0	37.714287	1
TCACGAC	135	0.0	37.48148	25
TGCGCGA	65	1.0913936E-11	37.230766	2
>>END_MODULE