FastQCFastQC Report
Thu 26 May 2016
SRR1546455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546455_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1563559
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147880.9457909807049174No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142140.9090798620327087No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACG207.857353E-444.01
ACGTCGA207.857353E-444.02
ATCGCGA302.5283043E-644.02
CGTTTTT189650.043.4663851
CGACGGT1350.042.3703728
GAGCGAT8800.041.257
TATTGCG750.041.0666661
GTTTTTA106700.040.7835042
ACCCGCT2800.040.0714334
GCGCGAC2750.040.09
CGACCCG2900.039.44827732
ACGAGGT1850.039.243245
AGCGATC1300.038.9230778
TAGTTCG404.1258136E-738.51
TGCGCGA800.038.52
AGGTCGC404.1258136E-738.58
TAGTGCG1150.038.260871
CGGTCTA1500.038.1333331
ATTGCGA1100.038.02
CGTGGCG3150.037.71428727
Produced by FastQC (version 0.11.5)