##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546455_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1563559
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59644887081332	31.0	31.0	34.0	30.0	34.0
2	31.9665954402744	31.0	31.0	34.0	30.0	34.0
3	32.00470081397632	33.0	31.0	34.0	30.0	34.0
4	35.724066056989216	37.0	35.0	37.0	35.0	37.0
5	35.68156686124412	37.0	35.0	37.0	33.0	37.0
6	35.72678421473062	37.0	35.0	37.0	33.0	37.0
7	36.095059412532564	37.0	35.0	37.0	35.0	37.0
8	18.225791927263376	25.0	0.0	37.0	0.0	37.0
9	27.8232916058812	25.0	17.0	39.0	17.0	39.0
10	33.65489054138667	32.0	32.0	39.0	27.0	39.0
11	35.82745006744229	37.0	35.0	39.0	32.0	39.0
12	36.68901077605642	38.0	35.0	39.0	33.0	39.0
13	36.95859254431716	39.0	37.0	39.0	33.0	39.0
14	38.17944829712214	40.0	37.0	41.0	33.0	41.0
15	38.36777953374321	40.0	37.0	41.0	33.0	41.0
16	38.397801426105445	40.0	37.0	41.0	34.0	41.0
17	38.33880141395368	40.0	37.0	41.0	33.0	41.0
18	38.2355760159994	40.0	37.0	41.0	33.0	41.0
19	38.19696986170653	40.0	37.0	41.0	34.0	41.0
20	38.04535549985642	40.0	36.0	41.0	34.0	41.0
21	37.95014642875645	40.0	36.0	41.0	33.0	41.0
22	37.94754978865524	40.0	36.0	41.0	33.0	41.0
23	37.76019708882108	39.0	36.0	41.0	33.0	41.0
24	37.5072932968951	39.0	35.0	41.0	33.0	41.0
25	37.383010810593014	39.0	35.0	41.0	33.0	41.0
26	37.34463617938306	39.0	35.0	41.0	33.0	41.0
27	37.208672010458194	39.0	35.0	41.0	33.0	41.0
28	37.15231148936497	39.0	35.0	41.0	33.0	41.0
29	37.01177633846884	39.0	35.0	41.0	32.0	41.0
30	36.83606247030013	39.0	35.0	41.0	32.0	41.0
31	36.70072891397127	39.0	35.0	41.0	31.0	41.0
32	36.29146133916277	39.0	35.0	41.0	30.0	41.0
33	35.93137003464532	39.0	35.0	41.0	27.0	41.0
34	35.59666952126527	39.0	35.0	41.0	24.0	41.0
35	35.30279062062896	39.0	35.0	41.0	22.0	41.0
36	35.14079737317236	39.0	35.0	41.0	21.0	41.0
37	35.078572027022965	39.0	35.0	41.0	21.0	41.0
38	34.98840849625758	39.0	35.0	41.0	20.0	41.0
39	34.89837927446294	39.0	35.0	41.0	19.0	41.0
40	34.851216999166645	39.0	35.0	41.0	19.0	41.0
41	34.816539702051536	39.0	35.0	41.0	18.0	41.0
42	34.648124567093404	39.0	34.0	40.0	18.0	41.0
43	34.66073553988049	39.0	34.0	40.0	18.0	41.0
44	34.42731038611271	38.0	34.0	40.0	18.0	41.0
45	34.35224382322637	38.0	34.0	40.0	18.0	41.0
46	34.34861748101606	38.0	34.0	40.0	18.0	41.0
47	34.249895910547664	38.0	34.0	40.0	18.0	41.0
48	34.22091203465939	38.0	34.0	40.0	18.0	41.0
49	34.17489074604796	38.0	34.0	40.0	18.0	41.0
50	33.60016091493829	38.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	0.0
13	8.0
14	11.0
15	25.0
16	68.0
17	171.0
18	496.0
19	1260.0
20	2383.0
21	4197.0
22	6668.0
23	10598.0
24	16281.0
25	26320.0
26	35914.0
27	39101.0
28	36471.0
29	34263.0
30	35888.0
31	42456.0
32	53442.0
33	68940.0
34	99651.0
35	143892.0
36	151445.0
37	233020.0
38	266637.0
39	253948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.444709793490365	21.541240209035927	26.400538770842672	21.613511226631037
2	38.27050977929199	22.87607950835242	27.18854868924038	11.664862023115214
3	26.001257387792847	23.100183619550013	39.116400468418526	11.782158524238612
4	23.16107035295758	25.106120076057252	38.58121119829824	13.151598372686928
5	21.077938216594323	29.328666203194125	36.784540909553144	12.808854670658414
6	18.245617850045953	37.49477953822017	34.08307585450885	10.176526757225023
7	80.09400348819584	2.6211994558567984	15.472457387281196	1.8123396686661648
8	40.69222843525572	50.562594695818966	7.443786898991339	1.3013899699339775
9	75.39785834752638	4.583645388501489	16.987910273932737	3.0305859900393908
10	34.16231814725252	33.77339774194642	22.094401298575878	9.969882812225187
11	25.372947231284527	22.79734886883066	37.40191447844309	14.427789421441723
12	24.95102519316508	20.77817338520644	37.13009870430217	17.140702717326306
13	20.697843829366207	24.299498771712486	37.84526199523011	17.157395403691194
14	16.60615301373341	27.62211083815833	37.6889519359359	18.08278421217236
15	16.68494760990791	24.645120523114254	42.94203160865692	15.72790025832092
16	19.58282354551379	23.64515825753937	40.652639267210255	16.119378929736584
17	19.08607222368967	23.85282550898303	36.89262765268212	20.16847461464518
18	19.844534168521943	24.156235869577035	38.518469721961246	17.480760239939777
19	21.421065658539266	26.965339971181134	34.49578813463387	17.11780623564573
20	22.99804484512577	24.97161923534705	34.58775780127261	17.442578118254573
21	21.510541015721184	26.380584295188093	35.46063819785502	16.6482364912357
22	19.635651740676238	23.230015624610264	36.731392931127	20.402939703586497
23	18.258153353982802	26.120728415109372	36.293737556433754	19.327380674474067
24	18.790912271299003	24.152142643801735	38.95043295456072	18.106512130338544
25	18.558493795245333	26.048905094083434	36.39382971797035	18.998771392700885
26	17.867953815621924	27.949440986876734	35.39642571850503	18.786179478996313
27	18.03871807843516	26.04174194897666	36.22453645817011	19.69500351441807
28	17.482998722785645	26.128786953354492	38.121298908451806	18.266915415408054
29	18.570645559265753	24.54541210149409	37.32580606168363	19.558136277556525
30	20.15363667120972	25.406972170541692	36.034585199535165	18.40480595871342
31	22.43983118001943	24.59421102753398	33.585173312935424	19.380784479511167
32	22.880236690780457	25.989745190299825	32.733142785145944	18.39687533377378
33	22.581367252530924	26.65265589593997	32.510637590266825	18.25533926126229
34	19.302757363169537	26.994312334871918	33.64190286391495	20.061027438043592
35	20.07177215570375	27.154523749983213	33.402193329449034	19.371510764864006
36	21.657321533757283	29.365761061782763	30.715246434576503	18.26167096988345
37	21.69396869577675	29.61186626152259	30.850450798466834	17.843714244233826
38	21.361522014839224	29.621203932822493	30.324855026257403	18.692419026080884
39	21.146243921719616	29.39805917141598	29.997908617455433	19.45778828940897
40	22.320999719230294	26.75773667639021	31.036053004715527	19.88521059966397
41	19.391017543949413	27.15561101307977	30.930908267612544	22.522463175358272
42	20.58655925360028	28.249013948306395	29.165129042140396	21.999297755952927
43	20.230512567802048	27.108347046705628	30.91664593405174	21.74449445144059
44	20.525032953665324	28.042433959959297	31.155651945337524	20.27688114103785
45	19.938294621437375	28.681936530696955	30.53207458113189	20.84769426673378
46	20.96428724467705	28.561314283631127	30.167457703866628	20.3069407678252
47	20.443680091381268	27.003138352949907	30.74511419140563	21.8080673642632
48	20.583681204227023	25.423281116990147	32.0261659457686	21.96687173301423
49	21.074868297262846	24.84434549639636	32.37031669415737	21.710469512183423
50	19.530890743489692	26.549813598335593	32.69393735701691	21.225358301157808
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	387.0
1	1031.5
2	1676.0
3	25436.0
4	49196.0
5	34291.5
6	19387.0
7	19235.5
8	19084.0
9	19210.0
10	19336.0
11	19027.0
12	18718.0
13	18003.5
14	17289.0
15	16433.5
16	15578.0
17	14782.5
18	13987.0
19	13186.5
20	12386.0
21	12321.0
22	12256.0
23	13108.0
24	13960.0
25	15823.5
26	17687.0
27	20203.0
28	22719.0
29	26895.0
30	31071.0
31	36412.5
32	41754.0
33	46707.0
34	51660.0
35	56390.0
36	61120.0
37	66477.0
38	71834.0
39	79147.0
40	86460.0
41	94340.0
42	102220.0
43	108843.0
44	115466.0
45	122199.0
46	128932.0
47	131508.5
48	134085.0
49	125959.0
50	117833.0
51	103794.0
52	89755.0
53	79511.5
54	69268.0
55	62855.5
56	56443.0
57	51938.5
58	47434.0
59	43193.5
60	38953.0
61	33895.5
62	28838.0
63	23259.0
64	17680.0
65	13222.0
66	8764.0
67	6619.0
68	4474.0
69	3606.0
70	2738.0
71	2216.0
72	1694.0
73	1255.0
74	816.0
75	572.5
76	329.0
77	247.5
78	166.0
79	115.0
80	64.0
81	44.0
82	24.0
83	21.5
84	19.0
85	16.5
86	14.0
87	8.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1563559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.41334667839545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.15745564024492	31.15927399424348
2	9.641328566149694	8.564073360974474
3	5.298001678544876	7.059059557558037
4	3.644672138909957	6.474883489379879
5	2.6516151196897786	5.888355078422858
6	1.9705635925448395	5.251159439251101
7	1.4590523691045658	4.536097908368269
8	1.1222686152432908	3.987496406006646
9	0.8909354596805342	3.561248289498046
>10	3.1271371130154666	19.326899770957766
>50	0.0235860219260064	0.6946367363439055
>100	0.011879940279071199	1.1482077752109572
>500	0.0010526212669347028	0.33286308473589027
>1k	1.5037446670495755E-4	0.07880787319188413
>5k	0.0	0.0
>10k+	3.007489334099151E-4	1.9369372358567998
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14788	0.9457909807049174	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14214	0.9090798620327087	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.031466673147607475	0.0
2	0.0	0.0	0.0	0.11032522597484329	0.0
3	0.0	0.0	0.0	0.21930736224216674	0.0
4	0.0	0.0	0.0	0.3187599572513733	0.0
5	0.0	0.0	0.0	0.5529052629289972	0.0
6	6.395665273903959E-5	0.0	0.0	1.053174200653765	0.0
7	6.395665273903959E-5	0.0	0.0	1.4266810526497562	0.0
8	3.197832636951979E-4	0.0	0.0	2.189108309951847	0.0
9	3.197832636951979E-4	0.0	0.0	3.0464472399186726	0.0
10	3.197832636951979E-4	0.0	0.0	3.9490035233719993	0.0
11	3.197832636951979E-4	0.0	0.0	4.389217164174809	0.0
12	3.197832636951979E-4	0.0	0.0	4.824889882633147	0.0
13	3.197832636951979E-4	0.0	0.0	5.120177748329293	0.0
14	3.197832636951979E-4	0.0	0.0	5.2861452621871	0.0
15	3.197832636951979E-4	0.0	0.0	5.405296506239931	0.0
16	3.8373991643423754E-4	0.0	0.0	5.568258057419004	0.0
17	3.8373991643423754E-4	0.0	0.0	5.743691155882189	0.0
18	3.8373991643423754E-4	0.0	0.0	6.0472933864344105	0.0
19	3.8373991643423754E-4	0.0	0.0	6.189916722042469	0.0
20	3.8373991643423754E-4	0.0	0.0	6.362535727785136	0.0
21	3.8373991643423754E-4	0.0	0.0	6.519229526995783	0.0
22	3.8373991643423754E-4	0.0	0.0	6.692168316002147	0.0
23	3.8373991643423754E-4	0.0	0.0	6.887427976814434	0.0
24	3.8373991643423754E-4	0.0	0.0	7.05154074774281	0.0
25	3.8373991643423754E-4	0.0	0.0	7.196338609543996	0.0
26	3.8373991643423754E-4	0.0	0.0	7.335955982473319	0.0
27	3.8373991643423754E-4	0.0	0.0	7.501156016498258	0.0
28	3.8373991643423754E-4	0.0	0.0	7.660663908429423	0.0
29	3.8373991643423754E-4	0.0	0.0	7.833538740783047	0.0
30	3.8373991643423754E-4	0.0	0.0	8.061288381186767	0.0
31	3.8373991643423754E-4	0.0	0.0	8.244460234631376	0.0
32	3.8373991643423754E-4	0.0	0.0	8.434539406571801	0.0
33	3.8373991643423754E-4	0.0	0.0	8.613681990893852	0.0
34	3.8373991643423754E-4	0.0	0.0	8.806319428943839	0.0
35	3.8373991643423754E-4	0.0	0.0	9.010085324570419	0.0
36	3.8373991643423754E-4	0.0	0.0	9.213467480280565	0.0
37	3.8373991643423754E-4	0.0	0.0	9.422861561348181	0.0
38	3.8373991643423754E-4	0.0	0.0	9.675298469709169	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCACG	20	7.857353E-4	44.0	1
ACGTCGA	20	7.857353E-4	44.0	2
ATCGCGA	30	2.5283043E-6	44.0	2
CGTTTTT	18965	0.0	43.466385	1
CGACGGT	135	0.0	42.37037	28
GAGCGAT	880	0.0	41.25	7
TATTGCG	75	0.0	41.066666	1
GTTTTTA	10670	0.0	40.783504	2
ACCCGCT	280	0.0	40.07143	34
GCGCGAC	275	0.0	40.0	9
CGACCCG	290	0.0	39.448277	32
ACGAGGT	185	0.0	39.24324	5
AGCGATC	130	0.0	38.923077	8
TAGTTCG	40	4.1258136E-7	38.5	1
TGCGCGA	80	0.0	38.5	2
AGGTCGC	40	4.1258136E-7	38.5	8
TAGTGCG	115	0.0	38.26087	1
CGGTCTA	150	0.0	38.13333	31
ATTGCGA	110	0.0	38.0	2
CGTGGCG	315	0.0	37.714287	27
>>END_MODULE