FastQCFastQC Report
Thu 26 May 2016
SRR1546454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546454_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1715363
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119240.6951298354925459No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109830.6402726420005561No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG254.443761E-544.01
ACGTGCG207.8575686E-444.01
TCGACGA207.8575686E-444.02
CGTTTTT145600.043.3200571
CGACGGT2650.042.33962228
AGCGATC1250.042.248
GAGCGAT9450.041.206357
TTAGCGA1550.041.1612932
CGGTCTA2750.040.80000331
CGAGGTT2700.040.740746
TCTAGCG650.040.6153871
CGAGATT3900.040.051286
TATTGCG1100.040.01
GTTTTTA84000.039.8880962
GAGACCG4450.039.5505647
TATACGA900.039.111112
CTAGCGA452.3521352E-839.111112
GCGCGAC1650.038.6666689
TACGACG404.1261228E-738.51
TATAGCG1200.038.51
Produced by FastQC (version 0.11.5)