##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546454_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1715363
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58020663847827	31.0	31.0	34.0	30.0	34.0
2	31.963280658379595	31.0	31.0	34.0	30.0	34.0
3	32.031117611840756	33.0	31.0	34.0	30.0	34.0
4	35.73373216048149	37.0	35.0	37.0	35.0	37.0
5	35.678388189555214	37.0	35.0	37.0	33.0	37.0
6	35.72160120044562	37.0	35.0	37.0	33.0	37.0
7	36.09098715548837	37.0	35.0	37.0	35.0	37.0
8	18.28766039607943	27.0	0.0	37.0	0.0	37.0
9	27.871094922765618	26.0	17.0	39.0	17.0	39.0
10	33.65579705286869	32.0	32.0	39.0	27.0	39.0
11	35.774969496252396	37.0	35.0	39.0	32.0	39.0
12	36.56626265111233	37.0	35.0	39.0	33.0	39.0
13	36.78918456326737	39.0	35.0	39.0	33.0	39.0
14	37.984367740239236	40.0	37.0	41.0	33.0	41.0
15	38.218477954811895	40.0	37.0	41.0	33.0	41.0
16	38.288780858628755	40.0	37.0	41.0	34.0	41.0
17	38.239103909784696	40.0	37.0	41.0	33.0	41.0
18	38.18277705651807	40.0	37.0	41.0	33.0	41.0
19	38.16418274149553	40.0	37.0	41.0	34.0	41.0
20	38.021633321926615	40.0	36.0	41.0	34.0	41.0
21	37.90794892976005	40.0	36.0	41.0	33.0	41.0
22	37.913959319397705	40.0	36.0	41.0	33.0	41.0
23	37.73306699514913	39.0	36.0	41.0	33.0	41.0
24	37.54801286957921	39.0	35.0	41.0	33.0	41.0
25	37.43415650215144	39.0	35.0	41.0	33.0	41.0
26	37.39344675150391	39.0	35.0	41.0	33.0	41.0
27	37.23729263135558	39.0	35.0	41.0	33.0	41.0
28	37.22038309092594	39.0	35.0	41.0	33.0	41.0
29	37.130940214986566	39.0	35.0	41.0	32.0	41.0
30	36.99980820386122	39.0	35.0	41.0	32.0	41.0
31	36.90801130722768	39.0	35.0	41.0	31.0	41.0
32	36.568439449842394	39.0	35.0	41.0	30.0	41.0
33	36.33784685807027	39.0	35.0	41.0	30.0	41.0
34	36.13198255995961	39.0	35.0	41.0	29.0	41.0
35	35.89943761174749	39.0	35.0	41.0	27.0	41.0
36	35.740491079730646	39.0	35.0	41.0	26.0	41.0
37	35.670755985759286	39.0	35.0	41.0	25.0	41.0
38	35.5775121650636	39.0	35.0	41.0	25.0	41.0
39	35.51066567251363	39.0	35.0	41.0	24.0	41.0
40	35.438813242444894	39.0	35.0	41.0	24.0	41.0
41	35.43946150173462	39.0	35.0	41.0	24.0	41.0
42	35.272674063740446	39.0	35.0	40.0	23.0	41.0
43	35.31900769691313	39.0	35.0	40.0	24.0	41.0
44	35.0890831853083	38.0	35.0	40.0	23.0	41.0
45	35.030380158602	38.0	35.0	40.0	23.0	41.0
46	35.04859496211589	38.0	35.0	40.0	23.0	41.0
47	34.954030721194286	38.0	34.0	40.0	23.0	41.0
48	34.94591348886504	38.0	34.0	40.0	23.0	41.0
49	34.918304172353025	38.0	34.0	40.0	23.0	41.0
50	34.31443840166775	38.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	4.0
13	2.0
14	11.0
15	26.0
16	60.0
17	172.0
18	548.0
19	1187.0
20	2288.0
21	3962.0
22	6336.0
23	9949.0
24	15191.0
25	22952.0
26	30871.0
27	33943.0
28	32941.0
29	33104.0
30	37625.0
31	46179.0
32	59561.0
33	77983.0
34	116059.0
35	176188.0
36	167456.0
37	262333.0
38	301959.0
39	276470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.428603158631727	23.294836136724413	28.280369810937977	17.99619089370588
2	34.68385408802685	24.479133571145002	28.882807895471686	11.954204445356464
3	26.936922389022033	24.234462326632904	36.55885080883755	12.269764475507516
4	23.71194901603917	26.543711156180937	36.2565824259938	13.487757401786094
5	22.374447857392283	30.23884740431034	34.018688755674454	13.36801598262292
6	19.107209377840142	39.475084865419156	31.08939623858041	10.328309518160296
7	83.59070354204911	2.7654204970026752	11.952164060901396	1.6917119000468124
8	42.78668713269436	50.52627344766093	5.541800773364005	1.1452386462806998
9	79.72458307658495	4.6050894183913265	12.670787465976588	2.9995400390471287
10	39.8411881333572	32.85596109977888	17.986630235116415	9.316220531747508
11	29.708930412979644	22.13362419499546	33.71560421904868	14.441841172976217
12	27.710169800794347	21.996218876121265	33.00980608769106	17.283805235393324
13	20.799037871284387	29.46425916846755	33.71700333981787	16.019699620430195
14	16.03963709139115	32.5022750286674	34.369751475343705	17.088336404597744
15	15.296762259649999	25.603676889381433	43.28599835719903	15.81356249376954
16	18.088124787581403	23.32765717810166	40.93022876207543	17.653989272241503
17	18.049590669729966	23.82959175404856	34.022303150994865	24.098514425226615
18	19.852882451119676	24.754818659374138	36.674802942584165	18.71749594692202
19	22.962428360644367	26.493984072175973	31.59966724244373	18.94392032473593
20	24.434128519736056	25.492038711339816	31.678192895614515	18.395639873309612
21	20.80101995904074	27.384932518656402	33.449887866300024	18.36415965600284
22	20.484410588312794	23.62357122078534	33.37468512495606	22.517333065945806
23	18.285925486325635	27.991859448991264	33.33207023819448	20.390144826488623
24	17.99665726729561	25.24719257673157	38.371061985130844	18.385088170841975
25	17.24177331561891	27.470103995480837	35.87573009328055	19.4123925956197
26	17.47892428599661	30.710525993623506	32.82488895936312	18.985660761016764
27	18.266221202159542	28.26690327353452	34.07960880583293	19.387266718473
28	16.620738584194715	27.13588902174059	37.88743257258085	18.35593982148385
29	17.095623491937275	24.43995818960768	37.27211091763085	21.1923074008242
30	18.89559236150016	27.109888694113142	34.082348750672594	19.9121701937141
31	22.82852084369314	25.87627225257861	30.37211365757568	20.923093246152565
32	23.216543670348493	27.298187030966623	30.02606445399603	19.45920484468885
33	21.795153562248924	27.71238507534557	29.625216353623113	20.867245008782398
34	18.56149398115734	28.354056838115316	31.08922134848426	21.99522783224309
35	19.407612266324968	28.089739606135844	31.503069612670902	20.99957851486828
36	22.915499518177786	28.802766528134278	29.250485174275063	19.03124877941287
37	20.733454085228608	30.135137577294135	30.01755313598346	19.113855201493795
38	19.775172951730916	31.62928196539158	28.675271648041843	19.920273434835657
39	20.850455559552117	30.119805545531765	29.38450928462372	19.6452296102924
40	22.139337271469653	27.372398728432408	29.460877959942007	21.027386040155932
41	19.318068537096813	26.201392941319124	29.952785503709713	24.52775301787435
42	20.939882695382845	27.687200901500148	28.532561329584468	22.840355073532542
43	20.900532423749375	27.222984289622666	29.713244368684645	22.163238917943314
44	20.03150353598626	29.395410767283657	30.368382668857848	20.204703027872235
45	18.943337357748767	30.830500599581544	28.367465078820054	21.85869696384963
46	20.89785077560843	29.59122937827154	29.162107379021236	20.3488124670988
47	20.42279097776972	27.3522863673753	29.96788434867722	22.25703830617776
48	20.770938862503154	25.412347124194707	31.41842280613491	22.39829120716723
49	20.308529448285874	25.127334564170965	32.16566989027979	22.398466097263377
50	19.247063158060424	28.61114527945397	30.794006866185175	21.347784696300433
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	573.0
1	1051.0
2	1529.0
3	18971.5
4	36414.0
5	25336.5
6	14259.0
7	14391.5
8	14524.0
9	14940.5
10	15357.0
11	15263.0
12	15169.0
13	14553.5
14	13938.0
15	13360.0
16	12782.0
17	12234.0
18	11686.0
19	11407.0
20	11128.0
21	11691.0
22	12254.0
23	13322.5
24	14391.0
25	16512.5
26	18634.0
27	21987.5
28	25341.0
29	30175.5
30	35010.0
31	40667.5
32	46325.0
33	51977.5
34	57630.0
35	63353.5
36	69077.0
37	76974.5
38	84872.0
39	94912.5
40	104953.0
41	117329.5
42	129706.0
43	138827.5
44	147949.0
45	153036.5
46	158124.0
47	155046.0
48	151968.0
49	142057.5
50	132147.0
51	117232.0
52	102317.0
53	90648.5
54	78980.0
55	69704.0
56	60428.0
57	53571.0
58	46714.0
59	40846.5
60	34979.0
61	29528.0
62	24077.0
63	19160.0
64	14243.0
65	10989.5
66	7736.0
67	5996.5
68	4257.0
69	3447.0
70	2637.0
71	2112.0
72	1587.0
73	1249.0
74	911.0
75	669.0
76	427.0
77	289.0
78	151.0
79	120.0
80	89.0
81	73.5
82	58.0
83	40.5
84	23.0
85	14.0
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1715363.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.24235068176558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.73871284283878	24.512481511436583
2	6.918834711191824	4.5999665955722175
3	3.2809650942065534	3.272009767087388
4	2.216372950026929	2.947097873854979
5	1.6601656248413472	2.759390394539427
6	1.350653696570776	2.6939342298617244
7	1.1561414602000666	2.69030019003894
8	0.9816981223070426	2.61071625962892
9	0.851452246461042	2.5473846749071547
>10	7.748537297729712	46.86143571325577
>50	0.07901456016462619	1.6811228763257418
>100	0.015451775759546895	0.995454960461031
>500	0.001454267419504954	0.36414566277706295
>1k	1.8178342743811925E-4	0.08031025320788528
>5k	0.0	0.0
>10k+	3.635668548762385E-4	1.3842490370451679
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11924	0.6951298354925459	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10983	0.6402726420005561	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02874027246711046	0.0
2	0.0	0.0	0.0	0.09910438781762228	0.0
3	0.0	0.0	0.0	0.1711591074309053	0.0
4	0.0	0.0	0.0	0.2890933289338758	0.0
5	0.0	0.0	0.0	0.5553343519709822	0.0
6	0.0	0.0	0.0	0.9583977268951237	0.0
7	0.0	0.0	0.0	1.2467331987456882	0.0
8	0.0	0.0	0.0	1.852202711612644	0.0
9	0.0	0.0	0.0	2.4883362880043465	0.0
10	0.0	0.0	0.0	3.2317357900339463	0.0
11	0.0	0.0	0.0	3.657010207168978	0.0
12	0.0	0.0	0.0	4.083625448374484	0.0
13	0.0	0.0	0.0	4.291045102406896	0.0
14	0.0	0.0	0.0	4.39492981951925	0.0
15	0.0	0.0	0.0	4.501029811182822	0.0
16	0.0	0.0	0.0	4.67090639124197	0.0
17	0.0	0.0	0.0	4.866316925338835	0.0
18	0.0	0.0	0.0	5.1636300887917015	0.0
19	0.0	0.0	0.0	5.3274438121843595	0.0
20	0.0	0.0	0.0	5.513235390993043	0.0
21	0.0	0.0	0.0	5.697977629224835	0.0
22	0.0	0.0	0.0	5.906563217231572	0.0
23	0.0	0.0	5.82966987162484E-5	6.143947374404135	0.0
24	0.0	0.0	5.82966987162484E-5	6.310617636033889	0.0
25	0.0	0.0	5.82966987162484E-5	6.469709326830531	0.0
26	0.0	0.0	5.82966987162484E-5	6.619473545832573	0.0
27	0.0	0.0	5.82966987162484E-5	6.779323093712526	0.0
28	0.0	0.0	5.82966987162484E-5	6.946284838835862	0.0
29	0.0	0.0	5.82966987162484E-5	7.1279373520356915	0.0
30	0.0	0.0	5.82966987162484E-5	7.3566353010995345	0.0
31	0.0	0.0	5.82966987162484E-5	7.543534517183827	0.0
32	0.0	0.0	5.82966987162484E-5	7.723962799710615	0.0
33	0.0	0.0	5.82966987162484E-5	7.910803719096191	0.0
34	0.0	0.0	5.82966987162484E-5	8.098927165853524	0.0
35	0.0	0.0	5.82966987162484E-5	8.32027973087912	0.0
36	0.0	0.0	5.82966987162484E-5	8.519771033886123	0.0
37	0.0	0.0	5.82966987162484E-5	8.724975413367316	0.0
38	0.0	0.0	5.82966987162484E-5	8.939915341534125	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	25	4.443761E-5	44.0	1
ACGTGCG	20	7.8575686E-4	44.0	1
TCGACGA	20	7.8575686E-4	44.0	2
CGTTTTT	14560	0.0	43.320057	1
CGACGGT	265	0.0	42.339622	28
AGCGATC	125	0.0	42.24	8
GAGCGAT	945	0.0	41.20635	7
TTAGCGA	155	0.0	41.161293	2
CGGTCTA	275	0.0	40.800003	31
CGAGGTT	270	0.0	40.74074	6
TCTAGCG	65	0.0	40.615387	1
CGAGATT	390	0.0	40.05128	6
TATTGCG	110	0.0	40.0	1
GTTTTTA	8400	0.0	39.888096	2
GAGACCG	445	0.0	39.550564	7
TATACGA	90	0.0	39.11111	2
CTAGCGA	45	2.3521352E-8	39.11111	2
GCGCGAC	165	0.0	38.666668	9
TACGACG	40	4.1261228E-7	38.5	1
TATAGCG	120	0.0	38.5	1
>>END_MODULE