FastQCFastQC Report
Thu 26 May 2016
SRR1546453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546453_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1920339
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124110.6462921390442No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113900.5931244431321762No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC31980.1665330965001492No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC20170.10503353834921854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT134350.042.951991
GTTTTTA71850.040.662492
AGACTTA16350.040.2324188
CGGTCTA4300.039.90697531
TAAGCGA1550.039.7419362
GCGCGAC14900.039.275179
TCTCACG4500.039.1111123
CACGACG4300.038.8837226
TAATGCG800.038.5000041
CGTTAGA1450.037.9310342
GTATCGA357.292758E-637.7142872
CGACCCG15400.037.28571332
TTACTGG28800.037.0486140
CGCGACC16150.036.9164110
AGCGATA2150.036.8372088
ACGACGG4550.036.74725327
CGACGGT4700.036.5106428
ACGTGGC15700.036.4331226
CGCTGAA15850.036.3659337
TCAGACG15850.036.3659322
Produced by FastQC (version 0.11.5)