##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546453_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1920339
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.537022890229277	31.0	31.0	34.0	30.0	34.0
2	31.9119921013946	31.0	31.0	34.0	30.0	34.0
3	31.995642436049053	33.0	31.0	34.0	30.0	34.0
4	35.698066851738155	37.0	35.0	37.0	35.0	37.0
5	35.664698264212724	37.0	35.0	37.0	33.0	37.0
6	35.70747144124032	37.0	35.0	37.0	33.0	37.0
7	36.02312039697158	37.0	35.0	37.0	35.0	37.0
8	18.35509563675997	28.0	0.0	37.0	0.0	37.0
9	27.872291298567596	27.0	17.0	39.0	17.0	39.0
10	33.67668729323312	32.0	32.0	39.0	27.0	39.0
11	35.87971655004663	37.0	35.0	39.0	32.0	39.0
12	36.53952869779763	37.0	35.0	39.0	33.0	39.0
13	36.70804061157952	39.0	35.0	39.0	33.0	39.0
14	37.79417592414673	40.0	36.0	41.0	33.0	41.0
15	38.025433530225655	40.0	37.0	41.0	33.0	41.0
16	38.09296379441338	40.0	37.0	41.0	33.0	41.0
17	38.011331332644914	40.0	36.0	41.0	33.0	41.0
18	37.971688332112194	40.0	36.0	41.0	33.0	41.0
19	37.958100627024706	40.0	36.0	41.0	33.0	41.0
20	37.85675810364732	40.0	36.0	41.0	33.0	41.0
21	37.74619012580591	40.0	36.0	41.0	33.0	41.0
22	37.749090655347835	40.0	35.0	41.0	33.0	41.0
23	37.63417709060744	39.0	35.0	41.0	33.0	41.0
24	37.45873775411529	39.0	35.0	41.0	33.0	41.0
25	37.31057589311054	39.0	35.0	41.0	33.0	41.0
26	37.270865716938516	39.0	35.0	41.0	33.0	41.0
27	37.16304517067039	39.0	35.0	41.0	32.0	41.0
28	37.11292849856197	39.0	35.0	41.0	32.0	41.0
29	36.97499035326575	39.0	35.0	41.0	32.0	41.0
30	36.81179729204062	39.0	35.0	40.0	31.0	41.0
31	36.78243216432099	39.0	35.0	41.0	31.0	41.0
32	36.532162290095656	39.0	35.0	41.0	31.0	41.0
33	36.35157334199847	39.0	35.0	41.0	30.0	41.0
34	36.11047216142566	39.0	35.0	41.0	30.0	41.0
35	35.90405339890509	39.0	35.0	41.0	29.0	41.0
36	35.77235738064998	39.0	35.0	41.0	27.0	41.0
37	35.72567603949094	39.0	35.0	41.0	27.0	41.0
38	35.62959560785882	39.0	35.0	40.0	27.0	41.0
39	35.56821894467591	38.0	35.0	40.0	27.0	41.0
40	35.48014282894843	38.0	35.0	40.0	26.0	41.0
41	35.440784673956	38.0	35.0	40.0	26.0	41.0
42	35.286777490849275	38.0	35.0	40.0	26.0	41.0
43	35.285214745938084	38.0	35.0	40.0	26.0	41.0
44	35.03823647803851	38.0	34.0	40.0	24.0	41.0
45	34.93846346921038	38.0	34.0	40.0	24.0	41.0
46	34.931169965303	38.0	34.0	40.0	24.0	41.0
47	34.81940428226475	37.0	34.0	40.0	24.0	41.0
48	34.801727715783514	37.0	34.0	40.0	24.0	41.0
49	34.75253952557335	37.0	34.0	40.0	24.0	41.0
50	34.1774332552742	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	3.0
12	6.0
13	17.0
14	23.0
15	41.0
16	99.0
17	234.0
18	482.0
19	1112.0
20	2272.0
21	3736.0
22	6199.0
23	9576.0
24	14819.0
25	21872.0
26	30091.0
27	35412.0
28	36932.0
29	38864.0
30	45445.0
31	56294.0
32	72440.0
33	97337.0
34	147865.0
35	211956.0
36	203993.0
37	301102.0
38	318773.0
39	263339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.772162102628755	22.407293712203938	26.407629069659055	17.41291511550825
2	36.201785205632966	23.115033335260076	28.089155091887424	12.594026367219538
3	29.64539073569823	23.804963602780553	33.79689731865051	12.752748342870712
4	25.863922984431394	25.834032428649316	33.1948681977505	15.107176389168785
5	22.323402274285947	30.363180667580046	32.242223899009495	15.071193159124508
6	20.346667958105314	39.27509674073171	29.633830276841742	10.744405024321226
7	83.36194807271008	3.7073662514795567	10.772368836960558	2.1583168388498075
8	42.59300050668137	50.51092541473147	5.245428020781747	1.6506460578054187
9	79.14894193160687	4.3060626274840015	12.654432368451612	3.8905630724575193
10	42.81827323196581	26.011396946060046	17.644749182305834	13.525580639668307
11	36.49761838925315	20.905163098807034	25.9218294269918	16.675389084948023
12	33.42592115246318	18.65535199774623	30.026156840016267	17.892570009774317
13	25.125980360759222	24.1345929026073	31.397268919706367	19.342157816927116
14	19.31200689045007	27.525921204537322	33.514030595639625	19.648041309372978
15	19.02362030870591	21.76626106119805	39.912640424425064	19.297478205670977
16	22.993388146572038	19.223220483466722	37.91872164237668	19.86466972758456
17	22.136612337717455	20.404782697221687	31.988414545556797	25.470190419504057
18	24.161463158327777	22.377455230560855	32.81670580038212	20.64437581072925
19	26.147779116083147	26.538699677504855	27.68599710780232	19.627524098609673
20	27.753693488493436	24.95090710546419	27.174889433584383	20.120509972457988
21	24.888626435228364	26.559164814129172	29.785001502338908	18.767207248303556
22	22.802744723718053	21.64477209492699	31.417369537357725	24.135113643997233
23	22.506703243541896	25.977548755714487	29.582381027516497	21.933366973227123
24	22.990055401676475	24.169065982620776	32.69125919954758	20.14961941615517
25	23.663842686109067	22.881376673597735	30.616208908947844	22.83857173134535
26	20.102075727254405	25.667343109732187	30.466495759342493	23.764085403670915
27	20.895373160676318	23.638534654558388	32.04387350358452	23.42221868118077
28	20.301467605459244	25.021727934494898	33.56423006562903	21.112574394416818
29	22.56679679994001	24.637681159420293	31.712317460615026	21.083204580024674
30	24.184011260511813	23.487727948034177	32.20160607059483	20.126654720859182
31	28.364054471632354	22.415417277886874	27.553989165454638	21.666539085026134
32	28.75138191746353	23.801422561329016	27.725156860325185	19.722038660882273
33	27.846489604179265	23.799443744047274	27.125002408428927	21.229064243344535
34	23.24969705869641	24.801246029997827	30.23648428740967	21.712572623896094
35	23.103629098820573	26.250156873343716	29.148447227286432	21.49776680054928
36	27.777543444152307	26.727728802050056	24.817284864807725	20.677442888989912
37	24.639399606007064	29.53837838006727	24.76953287935099	21.052689134574678
38	24.80015247307897	29.344818805429668	23.859329003889417	21.995699717601944
39	24.153443740922825	27.08506154382117	24.52853376409061	24.232960951165396
40	24.206194843722905	23.76205451225018	27.90434397260067	24.127406671426243
41	21.055501138080306	24.410689987549073	27.962406637578052	26.57140223679257
42	22.07105099672506	26.329830306003267	25.76138900475385	25.83772969251783
43	21.59368736457469	24.633671450717816	27.490614938299956	26.282026246407536
44	22.896842692878707	24.489009492594796	28.875057997572302	23.739089816954195
45	22.373862114970326	26.16392209917103	27.147915029585924	24.31430075627272
46	23.557246923590053	26.006970644245627	28.189554031866248	22.246228400298072
47	22.129374032397404	25.089163944491048	28.659575210418573	24.121886812692967
48	22.24320810023647	23.663686463692088	30.202167429813176	23.89093800625827
49	23.771792376241905	22.689847990380866	29.62518596976888	23.913173663608355
50	20.85251614428494	24.330079220387653	29.980696116675233	24.836708518652177
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	633.0
1	1524.0
2	2415.0
3	21125.5
4	39836.0
5	26918.0
6	14000.0
7	13678.0
8	13356.0
9	13301.5
10	13247.0
11	13097.0
12	12947.0
13	12309.0
14	11671.0
15	10924.5
16	10178.0
17	9442.0
18	8706.0
19	8566.0
20	8426.0
21	8562.0
22	8698.0
23	9364.5
24	10031.0
25	11193.0
26	12355.0
27	14247.0
28	16139.0
29	18671.0
30	21203.0
31	24173.5
32	27144.0
33	31015.5
34	34887.0
35	39791.5
36	44696.0
37	50040.5
38	55385.0
39	64672.0
40	73959.0
41	85925.5
42	97892.0
43	107115.5
44	116339.0
45	126363.5
46	136388.0
47	147066.0
48	157744.0
49	157786.0
50	157828.0
51	144708.5
52	131589.0
53	123392.0
54	115195.0
55	112182.5
56	109170.0
57	108373.0
58	107576.0
59	102436.5
60	97297.0
61	89947.0
62	82597.0
63	71221.0
64	59845.0
65	49537.0
66	39229.0
67	32902.0
68	26575.0
69	22754.5
70	18934.0
71	15772.5
72	12611.0
73	9767.5
74	6924.0
75	5184.0
76	3444.0
77	2612.5
78	1781.0
79	1346.0
80	911.0
81	612.0
82	313.0
83	226.0
84	139.0
85	93.5
86	48.0
87	39.0
88	30.0
89	21.5
90	13.0
91	12.0
92	11.0
93	7.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1920339.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.87830013935007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.80764445294288	23.93791586762942
2	6.876619071087036	4.521830915263563
3	3.328688489357043	3.283248576704419
4	2.2584383039186795	2.970144496097722
5	1.726629281476519	2.8384317872887666
6	1.4904366243974063	2.940181360537061
7	1.2195276250451228	2.806719669912362
8	1.1145361723337346	2.931524383212073
9	0.9519987719974233	2.8170091222221574
>10	8.11602310926757	44.760160814862566
>50	0.07551722109997457	1.6274041914699902
>100	0.02899483647682476	2.0222607888421478
>500	0.003922721855073497	0.7960402464334456
>1k	6.822124965345213E-4	0.4124874649735436
>5k	0.0	0.0
>10k+	3.4110624826726063E-4	1.3346403145507493
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12411	0.6462921390442	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11390	0.5931244431321762	No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	3198	0.1665330965001492	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	2017	0.10503353834921854	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02483936429974083	0.0
2	5.2074138993167355E-5	0.0	0.0	0.08540158794879446	0.0
3	5.2074138993167355E-5	0.0	0.0	0.15669108423044056	0.0
4	5.2074138993167355E-5	0.0	0.0	0.2319382150755674	0.0
5	5.2074138993167355E-5	0.0	0.0	0.42180052584465555	0.0
6	5.2074138993167355E-5	0.0	0.0	0.8054307078073194	0.0
7	5.2074138993167355E-5	0.0	0.0	1.08657898423143	0.0
8	5.2074138993167355E-5	0.0	0.0	1.7433900993522498	0.0
9	5.2074138993167355E-5	0.0	0.0	2.508984090829796	0.0
10	5.2074138993167355E-5	0.0	0.0	3.411637216137359	0.0
11	5.2074138993167355E-5	0.0	0.0	3.9738296207075936	0.0
12	5.2074138993167355E-5	0.0	0.0	4.429999078287739	0.0
13	5.2074138993167355E-5	0.0	0.0	4.643555122298719	0.0
14	5.2074138993167355E-5	0.0	0.0	4.773271802530699	0.0
15	5.2074138993167355E-5	0.0	0.0	4.870962887281881	0.0
16	5.2074138993167355E-5	0.0	0.0	5.01546862298792	0.0
17	5.2074138993167355E-5	0.0	0.0	5.1642444380914	0.0
18	5.2074138993167355E-5	0.0	0.0	5.391443906518589	0.0
19	5.2074138993167355E-5	0.0	0.0	5.5137660590135384	0.0
20	5.2074138993167355E-5	0.0	0.0	5.6540017153221385	0.0
21	5.2074138993167355E-5	0.0	0.0	5.811682208193449	0.0
22	5.2074138993167355E-5	0.0	0.0	5.973528632184213	0.0
23	5.2074138993167355E-5	0.0	0.0	6.157714861803046	0.0
24	5.2074138993167355E-5	0.0	0.0	6.304459785485792	0.0
25	5.2074138993167355E-5	0.0	0.0	6.427250605231681	0.0
26	5.2074138993167355E-5	0.0	0.0	6.553270021595146	0.0
27	5.2074138993167355E-5	0.0	0.0	6.6945992348226016	0.0
28	5.2074138993167355E-5	0.0	0.0	6.8299919962048365	0.0
29	1.0414827798633471E-4	0.0	0.0	6.972466840490142	0.0
30	1.0414827798633471E-4	0.0	0.0	7.167276194463581	0.0
31	1.0414827798633471E-4	0.0	0.0	7.31521882334317	0.0
32	1.0414827798633471E-4	0.0	0.0	7.493572749394768	0.0
33	1.0414827798633471E-4	0.0	0.0	7.659220585532034	0.0
34	1.0414827798633471E-4	0.0	0.0	7.82439975441836	0.0
35	1.0414827798633471E-4	0.0	0.0	8.020563036005623	0.0
36	1.0414827798633471E-4	0.0	0.0	8.208186158798004	0.0
37	1.0414827798633471E-4	0.0	0.0	8.412264709512227	0.0
38	1.0414827798633471E-4	0.0	0.0	8.691277946237618	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	13435	0.0	42.95199	1
GTTTTTA	7185	0.0	40.66249	2
AGACTTA	1635	0.0	40.232418	8
CGGTCTA	430	0.0	39.906975	31
TAAGCGA	155	0.0	39.741936	2
GCGCGAC	1490	0.0	39.27517	9
TCTCACG	450	0.0	39.11111	23
CACGACG	430	0.0	38.88372	26
TAATGCG	80	0.0	38.500004	1
CGTTAGA	145	0.0	37.931034	2
GTATCGA	35	7.292758E-6	37.714287	2
CGACCCG	1540	0.0	37.285713	32
TTACTGG	2880	0.0	37.04861	40
CGCGACC	1615	0.0	36.91641	10
AGCGATA	215	0.0	36.837208	8
ACGACGG	455	0.0	36.747253	27
CGACGGT	470	0.0	36.51064	28
ACGTGGC	1570	0.0	36.43312	26
CGCTGAA	1585	0.0	36.36593	37
TCAGACG	1585	0.0	36.36593	22
>>END_MODULE