FastQCFastQC Report
Thu 26 May 2016
SRR1546452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546452_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1844100
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129260.7009381269996204No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120050.650995065343528No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG207.857722E-444.031
CTATACG302.5285535E-644.01
ATCGCGA351.446715E-744.02
CGTTTTT168900.043.1793981
TTAGCGA1950.042.8717962
CGACGGT3250.041.9692328
GACGGTC3300.040.66666829
ACGGTCT3300.040.66666830
TGCGCGA650.040.6153872
GCGGAGT603.6379788E-1240.3333324
GTTTTTA92950.040.1183432
CGGTCTA3350.040.05970431
GAGCGAT8900.040.0449457
TCACGAC3550.039.66197225
CACGACG3450.039.53623226
CGAGATC3300.039.3333326
TACGAGC452.3523171E-839.111114
TATGCGA1700.038.8235322
TCGCGCG404.126341E-738.51
GTAGCGA1150.038.260872
Produced by FastQC (version 0.11.5)