##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546452_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1844100
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.566394447155794	31.0	31.0	34.0	30.0	34.0
2	31.946933463478118	31.0	31.0	34.0	30.0	34.0
3	32.00425844585435	33.0	31.0	34.0	30.0	34.0
4	35.714397266959494	37.0	35.0	37.0	35.0	37.0
5	35.65999837319018	37.0	35.0	37.0	33.0	37.0
6	35.70481101892522	37.0	35.0	37.0	33.0	37.0
7	36.080693563255785	37.0	35.0	37.0	35.0	37.0
8	18.298703974838674	27.0	0.0	37.0	0.0	37.0
9	27.87621007537552	26.0	17.0	39.0	17.0	39.0
10	33.65461254812646	32.0	32.0	39.0	27.0	39.0
11	35.77801583428231	37.0	35.0	39.0	32.0	39.0
12	36.53248956130362	37.0	35.0	39.0	33.0	39.0
13	36.715324548560275	39.0	35.0	39.0	33.0	39.0
14	37.90791985250257	40.0	37.0	41.0	33.0	41.0
15	38.15811344287186	40.0	37.0	41.0	33.0	41.0
16	38.22809934385337	40.0	37.0	41.0	33.0	41.0
17	38.196945935686784	40.0	37.0	41.0	33.0	41.0
18	38.12176563093108	40.0	37.0	41.0	33.0	41.0
19	38.10129927878098	40.0	37.0	41.0	34.0	41.0
20	37.967179654031774	40.0	36.0	41.0	34.0	41.0
21	37.835083780706036	40.0	36.0	41.0	33.0	41.0
22	37.837255571823654	40.0	36.0	41.0	33.0	41.0
23	37.67558754948213	39.0	36.0	41.0	33.0	41.0
24	37.47323464020389	39.0	35.0	41.0	33.0	41.0
25	37.34330567756629	39.0	35.0	41.0	33.0	41.0
26	37.299775500244024	39.0	35.0	41.0	33.0	41.0
27	37.144809934385336	39.0	35.0	41.0	32.0	41.0
28	37.130383384848976	39.0	35.0	41.0	32.0	41.0
29	37.04267284854401	39.0	35.0	41.0	32.0	41.0
30	36.91702781844803	39.0	35.0	41.0	32.0	41.0
31	36.80382354536088	39.0	35.0	41.0	31.0	41.0
32	36.45576649856299	39.0	35.0	41.0	30.0	41.0
33	36.223717260452254	39.0	35.0	41.0	30.0	41.0
34	36.00965836993655	39.0	35.0	41.0	29.0	41.0
35	35.77386150425682	39.0	35.0	41.0	26.0	41.0
36	35.621103519331925	39.0	35.0	41.0	25.0	41.0
37	35.550075375521935	39.0	35.0	41.0	25.0	41.0
38	35.48871915839705	39.0	35.0	41.0	24.0	41.0
39	35.4221289517922	39.0	35.0	41.0	24.0	41.0
40	35.3607635160783	39.0	35.0	41.0	24.0	41.0
41	35.36174448240334	39.0	35.0	41.0	24.0	41.0
42	35.19467870505938	39.0	35.0	40.0	23.0	41.0
43	35.21480722303563	39.0	35.0	40.0	23.0	41.0
44	34.973237893823544	38.0	35.0	40.0	23.0	41.0
45	34.91275147768559	38.0	34.0	40.0	23.0	41.0
46	34.92537986009435	38.0	34.0	40.0	23.0	41.0
47	34.81425736131446	38.0	34.0	40.0	23.0	41.0
48	34.82519765739385	38.0	34.0	40.0	23.0	41.0
49	34.79714332194566	38.0	34.0	40.0	23.0	41.0
50	34.21660322108345	38.0	34.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	2.0
13	3.0
14	11.0
15	31.0
16	75.0
17	223.0
18	555.0
19	1395.0
20	2690.0
21	4484.0
22	7118.0
23	11049.0
24	16837.0
25	25600.0
26	34325.0
27	37888.0
28	36421.0
29	35866.0
30	40731.0
31	50255.0
32	64798.0
33	85589.0
34	127040.0
35	194491.0
36	180924.0
37	278397.0
38	319871.0
39	287427.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.577788623176616	23.032698877501222	28.945881459790684	18.44363103953148
2	35.68157909007104	23.709614446071253	28.367279431701103	12.241527032156608
3	26.14478607450789	24.319559676807113	37.0426766444336	12.492977604251395
4	23.504311046038715	26.196193265007324	36.94479691990673	13.354698769047232
5	21.8885635269237	30.24624478065181	34.8625345697088	13.00265712271569
6	18.902066048478932	38.65121197332032	31.863944471557943	10.582777506642808
7	83.21604034488368	3.1163169025540913	12.06729569979936	1.6003470527628654
8	42.77794045876037	50.32015617374329	5.785640691936446	1.1162626755598937
9	79.83043218914375	4.474757334200966	13.04956347269671	2.6452470039585707
10	39.53408166585326	33.29982105091915	18.0971205466081	9.06897673661949
11	30.885743723225424	22.26256710590532	33.38029391030855	13.471395260560707
12	28.92695623881568	22.072393037253946	32.573613144623394	16.42703757930698
13	20.271948375901523	30.56114093595792	33.29271731467925	15.87419337346131
14	15.984653760642049	32.793124017135725	34.59340599750556	16.628816224716665
15	15.402093161976033	24.957431809554798	44.34759503280733	15.29287999566184
16	17.654140231006995	23.32780217992517	41.849682772083945	17.168374816983896
17	18.46591833414674	22.77175858142183	34.09375847296784	24.668564611463587
18	20.02863185293639	24.234531749905102	36.88281546553874	18.85402093161976
19	23.08627514776856	25.887316306057155	32.18784230790087	18.838566238273412
20	24.504961769969093	25.383764437937206	31.729732660918604	18.3815411311751
21	21.156444878260398	26.80706035464454	33.854346293584946	18.18214847351011
22	20.80879561845887	23.315980695190067	33.330676210617646	22.54454747573342
23	17.842253673878858	27.5283336044683	33.36565262187517	21.26376009977767
24	17.86611355132585	24.610487500677838	39.611192451602406	17.912206496393903
25	17.277154167344506	27.24830540643132	36.58153028577626	18.893010140447917
26	17.81020552030801	30.65929179545578	32.8910037416626	18.639498942573614
27	18.04153787755545	28.90407244726425	34.23859877446993	18.815790900710375
28	16.54123962908736	26.702890298790738	38.50604630985304	18.249823762268857
29	17.001138766878153	23.811290060191965	37.96323409793395	21.224337074995933
30	18.637763678759285	26.72891925600564	34.74019847079876	19.89311859443631
31	23.221734179274442	25.560219077056555	30.423946640637713	20.794100103031287
32	23.683097445908572	26.469714223740574	30.531207635160783	19.315980695190067
33	22.575782224391304	26.438316794100103	30.27606962746055	20.709831354048045
34	18.67555989371509	27.38609619868771	31.3364784989968	22.601865408600403
35	19.303779621495583	26.883737324440105	32.38376443793721	21.428718616127107
36	23.45778428501708	26.37053305135296	30.38539124776314	19.78629141586682
37	19.603492218426332	29.44509516837482	31.67274009001681	19.27867252318204
38	19.245810964698226	29.890678379697412	30.34710698985955	20.51640366574481
39	19.644433599045605	29.10113334417873	31.185402093161972	20.069030963613688
40	21.624694973157638	26.118377528333603	30.536630334580554	21.720297163928205
41	18.524754622851255	25.495200911013505	30.888509299929506	25.091535166205738
42	20.45664551813893	26.831408275039315	29.310503768776098	23.40144243804566
43	20.728973482999837	26.81307955100049	30.20443576812537	22.2535111978743
44	20.060463098530448	29.01279757063066	30.425681904452034	20.501057426386858
45	18.955100048804294	30.864595195488313	28.20899083563798	21.97131392006941
46	21.269996204110406	28.935307195922128	29.306762106176453	20.48793449379101
47	20.49948484355512	26.60029282576867	30.2346944308877	22.665527899788515
48	21.153299712596933	24.992137085841332	31.69009272816008	22.16447047340166
49	20.59503280733149	24.54595737758256	32.458435008947454	22.400574806138497
50	19.233826798980534	28.326121143105038	30.83460766769698	21.60544439021745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	443.0
1	1035.5
2	1628.0
3	20204.5
4	38781.0
5	27232.5
6	15684.0
7	15912.0
8	16140.0
9	16718.0
10	17296.0
11	17311.5
12	17327.0
13	16848.5
14	16370.0
15	15484.0
16	14598.0
17	13838.5
18	13079.0
19	12741.0
20	12403.0
21	12561.0
22	12719.0
23	13953.5
24	15188.0
25	16963.5
26	18739.0
27	22270.0
28	25801.0
29	30299.0
30	34797.0
31	39941.5
32	45086.0
33	50780.0
34	56474.0
35	63773.5
36	71073.0
37	79330.5
38	87588.0
39	101815.5
40	116043.0
41	131784.5
42	147526.0
43	156805.5
44	166085.0
45	168678.5
46	171272.0
47	169100.5
48	166929.0
49	155204.5
50	143480.0
51	127253.5
52	111027.0
53	98154.0
54	85281.0
55	73938.5
56	62596.0
57	54872.0
58	47148.0
59	41875.5
60	36603.0
61	30806.0
62	25009.0
63	20101.0
64	15193.0
65	11752.0
66	8311.0
67	6356.5
68	4402.0
69	3495.5
70	2589.0
71	2175.5
72	1762.0
73	1314.5
74	867.0
75	603.0
76	339.0
77	260.0
78	181.0
79	156.5
80	132.0
81	93.0
82	54.0
83	46.5
84	39.0
85	21.5
86	4.0
87	2.0
88	0.0
89	1.0
90	2.0
91	2.5
92	3.0
93	5.5
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1844100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.80699090439601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5364733515484	25.198538766415517
2	7.088561498440553	4.792858682060631
3	3.301682878500875	3.3485988912803735
4	2.182465327278796	2.951303418738957
5	1.5714628793221133	2.656321563391932
6	1.2423221064468055	2.519950329178635
7	1.0846036294205808	2.5667029524287535
8	0.9032277596706934	2.4428330124628093
9	0.7917483648350827	2.408996679169505
>10	7.16920490758257	45.86930261383679
>50	0.10891704735587539	2.3515927181450125
>100	0.01763464864465303	1.1023393135267578
>500	0.0010173605717973125	0.21593636630468982
>1k	3.391201905991042E-4	0.14560084162832815
>5k	0.0	0.0
>10k+	3.391201905991042E-4	1.4291238514314366
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12926	0.7009381269996204	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12005	0.650995065343528	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02743885906404208	0.0
2	0.0	0.0	0.0	0.0975543625616832	0.0
3	0.0	0.0	0.0	0.1735806084268749	0.0
4	0.0	0.0	0.0	0.28507130849737	0.0
5	0.0	0.0	0.0	0.5288216474160837	0.0
6	0.0	0.0	0.0	0.953744373949352	0.0
7	0.0	0.0	0.0	1.2504202592050322	0.0
8	0.0	0.0	0.0	1.855756195434087	0.0
9	0.0	0.0	0.0	2.512445095168375	0.0
10	0.0	0.0	0.0	3.224120167019142	0.0
11	0.0	0.0	0.0	3.662816550078629	0.0
12	0.0	0.0	0.0	4.059432785640692	0.0
13	0.0	0.0	0.0	4.278835204164634	0.0
14	0.0	0.0	0.0	4.405129873651103	0.0
15	0.0	0.0	0.0	4.5079984816441625	0.0
16	0.0	0.0	0.0	4.68553766064747	0.0
17	0.0	0.0	0.0	4.884767637329863	0.0
18	0.0	0.0	0.0	5.183558375359254	0.0
19	0.0	0.0	0.0	5.357789707716501	0.0
20	0.0	0.0	0.0	5.546608101512933	0.0
21	0.0	0.0	0.0	5.739927335827775	0.0
22	0.0	0.0	0.0	5.953744373949352	0.0
23	0.0	0.0	0.0	6.194403774198796	0.0
24	0.0	0.0	0.0	6.375738842795943	0.0
25	0.0	0.0	0.0	6.537172604522532	0.0
26	0.0	0.0	0.0	6.686188384577843	0.0
27	0.0	0.0	0.0	6.8484355512173964	0.0
28	0.0	0.0	0.0	7.021690797679085	0.0
29	0.0	0.0	0.0	7.205411854020932	0.0
30	0.0	0.0	0.0	7.445149395369015	0.0
31	0.0	0.0	0.0	7.6476329917032695	0.0
32	0.0	0.0	0.0	7.844856569600347	0.0
33	0.0	0.0	0.0	8.03221083455344	0.0
34	0.0	0.0	0.0	8.226180792798655	0.0
35	0.0	0.0	0.0	8.461525947616723	0.0
36	0.0	0.0	0.0	8.67322813296459	0.0
37	0.0	0.0	0.0	8.88818393796432	0.0
38	0.0	0.0	0.0	9.113171736890624	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCG	20	7.857722E-4	44.0	31
CTATACG	30	2.5285535E-6	44.0	1
ATCGCGA	35	1.446715E-7	44.0	2
CGTTTTT	16890	0.0	43.179398	1
TTAGCGA	195	0.0	42.871796	2
CGACGGT	325	0.0	41.96923	28
GACGGTC	330	0.0	40.666668	29
ACGGTCT	330	0.0	40.666668	30
TGCGCGA	65	0.0	40.615387	2
GCGGAGT	60	3.6379788E-12	40.333332	4
GTTTTTA	9295	0.0	40.118343	2
CGGTCTA	335	0.0	40.059704	31
GAGCGAT	890	0.0	40.044945	7
TCACGAC	355	0.0	39.661972	25
CACGACG	345	0.0	39.536232	26
CGAGATC	330	0.0	39.333332	6
TACGAGC	45	2.3523171E-8	39.11111	4
TATGCGA	170	0.0	38.823532	2
TCGCGCG	40	4.126341E-7	38.5	1
GTAGCGA	115	0.0	38.26087	2
>>END_MODULE