FastQCFastQC Report
Thu 26 May 2016
SRR1546451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546451_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1209933
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83020.6861536961137518No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78820.6514410302058048No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC12230.10107997715575985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA502.7284841E-1144.02
ATTCGCG302.5278277E-644.01
TATTCGC207.856647E-444.044
CGTTTTT96800.043.01
GTTTTTA54250.040.715212
TAAGCGA650.040.6153872
CGTTAGA557.8216544E-1140.02
TCGTTAG452.3510438E-839.111111
CGTAAGA452.3510438E-839.111112
AGACTTA6900.038.8985528
TCACGAC2950.038.7796625
CGGTCTA2950.038.7796631
TACGATG404.1247768E-738.51
CGACGGT3050.038.22950728
CACGACG3050.038.22950726
TTCGCGA357.2905277E-637.7142872
TCGACGA357.2905277E-637.7142872
GAGCGAT6450.037.519387
GACCGAT4000.036.8500029
TCGTTCA1450.036.4137916
Produced by FastQC (version 0.11.5)