##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546451_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1209933
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.580237914000197	31.0	31.0	34.0	30.0	34.0
2	31.963521120590975	31.0	31.0	34.0	30.0	34.0
3	32.03834427195555	33.0	31.0	34.0	30.0	34.0
4	35.74070795655627	37.0	35.0	37.0	35.0	37.0
5	35.702442201345036	37.0	35.0	37.0	33.0	37.0
6	35.741622056758516	37.0	35.0	37.0	35.0	37.0
7	36.06284645513429	37.0	35.0	37.0	35.0	37.0
8	18.772736176300672	32.0	0.0	37.0	0.0	37.0
9	28.11526836609961	32.0	17.0	39.0	17.0	39.0
10	33.80838691068018	33.0	32.0	39.0	27.0	39.0
11	35.94611106565405	37.0	35.0	39.0	32.0	39.0
12	36.594130418791785	37.0	35.0	39.0	33.0	39.0
13	36.76355467616802	39.0	35.0	39.0	33.0	39.0
14	37.903064053960016	40.0	37.0	41.0	33.0	41.0
15	38.13714561054207	40.0	37.0	41.0	33.0	41.0
16	38.199167226615025	40.0	37.0	41.0	33.0	41.0
17	38.14363109362254	40.0	37.0	41.0	33.0	41.0
18	38.085062561315375	40.0	37.0	41.0	33.0	41.0
19	38.05104414872559	40.0	37.0	41.0	34.0	41.0
20	37.92368916295365	40.0	36.0	41.0	33.0	41.0
21	37.79775409051575	40.0	36.0	41.0	33.0	41.0
22	37.79785079008507	40.0	36.0	41.0	33.0	41.0
23	37.66058037924414	39.0	35.0	41.0	33.0	41.0
24	37.466529964882355	39.0	35.0	41.0	33.0	41.0
25	37.32639327962788	39.0	35.0	41.0	33.0	41.0
26	37.30387137138999	39.0	35.0	41.0	33.0	41.0
27	37.17822805064413	39.0	35.0	41.0	32.0	41.0
28	37.136221592435284	39.0	35.0	41.0	32.0	41.0
29	36.983238741318736	39.0	35.0	41.0	32.0	41.0
30	36.840133296637084	39.0	35.0	40.0	32.0	41.0
31	36.79519196517493	39.0	35.0	41.0	31.0	41.0
32	36.51564012222164	39.0	35.0	41.0	31.0	41.0
33	36.30202994711277	39.0	35.0	41.0	30.0	41.0
34	36.04047083598844	39.0	35.0	41.0	29.0	41.0
35	35.8002856356509	39.0	35.0	41.0	27.0	41.0
36	35.663762373619036	39.0	35.0	41.0	26.0	41.0
37	35.578072504841174	39.0	35.0	41.0	26.0	41.0
38	35.474971754634346	39.0	35.0	40.0	26.0	41.0
39	35.39896258718458	38.0	35.0	40.0	25.0	41.0
40	35.30253906621276	38.0	35.0	40.0	25.0	41.0
41	35.26876116280819	38.0	35.0	40.0	25.0	41.0
42	35.12025293962558	38.0	35.0	40.0	24.0	41.0
43	35.119119819031305	38.0	35.0	40.0	24.0	41.0
44	34.8625155277193	38.0	34.0	40.0	23.0	41.0
45	34.7745288375472	38.0	34.0	40.0	23.0	41.0
46	34.76951203083146	38.0	34.0	40.0	23.0	41.0
47	34.6504748610047	38.0	34.0	40.0	23.0	41.0
48	34.61799207063532	37.0	34.0	40.0	23.0	41.0
49	34.58377447346258	37.0	34.0	40.0	24.0	41.0
50	33.99084081515257	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	2.0
13	4.0
14	25.0
15	28.0
16	64.0
17	128.0
18	308.0
19	721.0
20	1391.0
21	2516.0
22	4059.0
23	6463.0
24	9927.0
25	14864.0
26	20803.0
27	23655.0
28	23586.0
29	24298.0
30	27542.0
31	34063.0
32	43934.0
33	58791.0
34	89764.0
35	134147.0
36	126984.0
37	189761.0
38	201016.0
39	171084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.48568309154308	22.104529754953372	26.998519752746642	18.411267400756902
2	35.72495336518634	23.904546780689508	27.94939885101076	12.421101003113396
3	28.556374609172575	24.25894656976874	34.64960456488087	12.535074256177822
4	25.706877984152843	25.856720991988812	34.307684805687586	14.128716218170759
5	22.163045391769625	29.89611821481024	32.928682827892125	15.012153565528008
6	19.85209098355033	38.569573687138046	30.808813380575618	10.769521948736005
7	82.57564675068785	3.583008315336469	11.661637462570242	2.1797074714054414
8	43.39769226891076	49.4614164585973	5.759161870946573	1.381729401545375
9	78.75469137547285	4.711665852572002	12.886829270711683	3.6468135012434573
10	41.448576078179535	28.059818188279845	18.137533235311377	12.35407249822924
11	35.423283768605366	21.918569044732227	27.20820078467155	15.449946401990855
12	31.903750042357714	20.076896819906555	30.65417671887617	17.36517641885956
13	23.401130475819734	26.692387099120364	31.76977568179395	18.136706743265947
14	18.252498278830316	29.957030678558233	33.51995523719082	18.270515805420633
15	17.76924837986897	22.892094025041057	41.271376183639916	18.067281411450058
16	21.368290640886727	20.28492486774061	39.06373328109903	19.283051210273626
17	20.810739107041464	20.951490702377733	31.93251196553859	26.30525822504221
18	23.097642596738826	22.714150287660555	33.9465904310404	20.24161668456022
19	26.27004966390701	25.119076841444937	29.28782006937574	19.323053425272306
20	27.85088099919582	24.273327531359172	28.354710550088313	19.52108091935669
21	24.61574318577971	26.028383389824068	30.750132445350282	18.605740979045947
22	22.742829561636885	22.248091423244094	31.66373675236563	23.34534226275339
23	21.61623825451492	26.275091265384116	30.31688531513728	21.791785164963677
24	21.84930901132542	24.107615876251	34.467858964091405	19.57521614833218
25	21.95394290427652	24.166710057499053	32.25533975848249	21.624007279741935
26	19.66513848287467	27.760049523403364	30.31143046763746	22.2633815260845
27	20.344349645806833	25.286028234621256	32.47948440120238	21.89013771836953
28	19.627698393216814	25.37066102007301	35.190378310203954	19.81126227650622
29	20.59626442125308	24.330107534880028	34.510588602839995	20.563039441026902
30	22.20436999404099	24.593262602144087	33.263494755494726	19.938872648320196
31	27.105633121834018	23.080534211398483	28.773080823483614	21.040751843283882
32	27.03827402013169	24.333000257039025	28.97598462063602	19.65274110219326
33	26.885372991727642	24.15910633068112	28.245861547705537	20.709659129885704
34	21.948901302799413	25.044031363720137	30.927910884321697	22.079156449158756
35	22.07775141268153	25.355618864846235	31.1677588759047	21.398870846567537
36	26.992899606837735	25.312889226097646	27.511688663752455	20.182522503312168
37	23.82900540773745	27.846831188173226	27.822201725219497	20.501961678869822
38	23.219880770257525	29.07888288029172	26.537419840602745	21.16381650884801
39	23.727760132172605	26.191367621182334	27.284651298873573	22.796220947771488
40	23.838758013873495	23.914712632848264	29.239966179945505	23.00656317333274
41	21.028850357829732	23.832972569555505	29.290630142330194	25.847546930284572
42	22.308094745742117	26.22211312527223	26.633623514690484	24.836168614295172
43	21.912370354391523	25.3253692559836	27.944522547942736	24.817737841682142
44	22.16676460597405	25.761343809946503	29.320466505170124	22.751425078909328
45	21.59590655019741	27.7812077197663	27.204233622853497	23.418652107182794
46	23.150124841623462	27.045464500926915	28.732004168825874	21.07240648862375
47	22.148747079383735	25.350411964960045	29.55014864459437	22.95069231106185
48	22.329170292900518	23.92487848500702	30.696079865579335	23.04987135651313
49	22.774649505385835	23.48535001524878	30.66599555512578	23.074004924239606
50	20.693955781022584	25.839447308239382	30.202746763663775	23.26385014707426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	491.0
1	1061.0
2	1631.0
3	13879.5
4	26128.0
5	17913.0
6	9698.0
7	9599.5
8	9501.0
9	9529.5
10	9558.0
11	9476.0
12	9394.0
13	8979.5
14	8565.0
15	8120.0
16	7675.0
17	7240.5
18	6806.0
19	6504.0
20	6202.0
21	6460.0
22	6718.0
23	7142.0
24	7566.0
25	8586.5
26	9607.0
27	11074.0
28	12541.0
29	14797.5
30	17054.0
31	18807.0
32	20560.0
33	23420.0
34	26280.0
35	30046.0
36	33812.0
37	36936.0
38	40060.0
39	47465.0
40	54870.0
41	63410.0
42	71950.0
43	77436.0
44	82922.0
45	87384.0
46	91846.0
47	94962.0
48	98078.0
49	94970.0
50	91862.0
51	83081.0
52	74300.0
53	69234.5
54	64169.0
55	62279.5
56	60390.0
57	58842.0
58	57294.0
59	54531.5
60	51769.0
61	48336.0
62	44903.0
63	39093.0
64	33283.0
65	27693.0
66	22103.0
67	18542.0
68	14981.0
69	12718.0
70	10455.0
71	8710.5
72	6966.0
73	5528.5
74	4091.0
75	3051.5
76	2012.0
77	1525.0
78	1038.0
79	758.5
80	479.0
81	341.0
82	203.0
83	148.5
84	94.0
85	54.5
86	15.0
87	10.0
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	3.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1209933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.94687152308922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.00971607243238	25.166302690236815
2	7.73912331271607	5.563945428470984
3	4.092759093563549	4.413656559338518
4	2.874650550357315	4.133387760298885
5	2.3544900336732684	4.231827537142349
6	1.8792656583259337	4.053223270655761
7	1.6122230748289466	4.0568063016205205
8	1.3966351822269267	4.0163732368110105
9	1.2497518764974367	4.043220312617373
>10	6.733521062386662	36.08891900935134
>50	0.03426152384912645	0.8362565121261812
>100	0.02198277587374444	1.6876432225984845
>500	6.941928294308385E-4	0.17126803490085496
>1k	4.6279521962055905E-4	0.19098174265777706
>5k	4.6279521962055905E-4	1.3461883811731115
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8302	0.6861536961137518	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7882	0.6514410302058048	No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1223	0.10107997715575985	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03099345170352408	0.0
2	0.0	0.0	0.0	0.10529508658743914	0.0
3	0.0	0.0	0.0	0.19075436408462287	0.0
4	0.0	0.0	0.0	0.2751392019227511	0.0
5	0.0	0.0	0.0	0.4930025050973897	0.0
6	0.0	0.0	0.0	0.841782148267714	0.0
7	0.0	0.0	0.0	1.053694708715276	0.0
8	0.0	0.0	0.0	1.4863632944964722	0.0
9	0.0	0.0	0.0	1.959612639708149	0.0
10	0.0	0.0	0.0	2.5627038852564565	0.0
11	0.0	0.0	0.0	2.9614036479705903	0.0
12	0.0	0.0	0.0	3.2929096073914836	0.0
13	0.0	0.0	0.0	3.478539720794457	0.0
14	8.264920454273088E-5	0.0	0.0	3.5726771647686277	0.0
15	8.264920454273088E-5	0.0	0.0	3.6601200231748368	0.0
16	8.264920454273088E-5	0.0	0.0	3.7947555773749455	0.0
17	8.264920454273088E-5	0.0	0.0	3.9492269406653095	0.0
18	8.264920454273088E-5	0.0	0.0	4.197257203498045	0.0
19	8.264920454273088E-5	0.0	0.0	4.315693513607778	0.0
20	8.264920454273088E-5	0.0	0.0	4.458263391443989	0.0
21	8.264920454273088E-5	0.0	0.0	4.602238305757426	0.0
22	8.264920454273088E-5	0.0	0.0	4.765222537115691	0.0
23	8.264920454273088E-5	0.0	0.0	4.936306390519144	0.0
24	8.264920454273088E-5	0.0	0.0	5.075735598582732	0.0
25	8.264920454273088E-5	0.0	0.0	5.19615548960149	0.0
26	8.264920454273088E-5	0.0	0.0	5.312194972779484	0.0
27	8.264920454273088E-5	0.0	0.0	5.441127731866144	0.0
28	8.264920454273088E-5	0.0	0.0	5.574606197202655	0.0
29	8.264920454273088E-5	0.0	0.0	5.714861897311669	0.0
30	8.264920454273088E-5	0.0	0.0	5.902640890032754	0.0
31	8.264920454273088E-5	0.0	0.0	6.05595516445952	0.0
32	8.264920454273088E-5	0.0	0.0	6.200591272409299	0.0
33	8.264920454273088E-5	0.0	0.0	6.352665808767924	0.0
34	8.264920454273088E-5	0.0	0.0	6.509616648194569	0.0
35	8.264920454273088E-5	0.0	0.0	6.696899745688397	0.0
36	8.264920454273088E-5	0.0	0.0	6.851949653410561	0.0
37	8.264920454273088E-5	0.0	0.0	7.029810741586518	0.0
38	1.6529840908546177E-4	0.0	0.0	7.252798295442806	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	50	2.7284841E-11	44.0	2
ATTCGCG	30	2.5278277E-6	44.0	1
TATTCGC	20	7.856647E-4	44.0	44
CGTTTTT	9680	0.0	43.0	1
GTTTTTA	5425	0.0	40.71521	2
TAAGCGA	65	0.0	40.615387	2
CGTTAGA	55	7.8216544E-11	40.0	2
TCGTTAG	45	2.3510438E-8	39.11111	1
CGTAAGA	45	2.3510438E-8	39.11111	2
AGACTTA	690	0.0	38.898552	8
TCACGAC	295	0.0	38.77966	25
CGGTCTA	295	0.0	38.77966	31
TACGATG	40	4.1247768E-7	38.5	1
CGACGGT	305	0.0	38.229507	28
CACGACG	305	0.0	38.229507	26
TTCGCGA	35	7.2905277E-6	37.714287	2
TCGACGA	35	7.2905277E-6	37.714287	2
GAGCGAT	645	0.0	37.51938	7
GACCGAT	400	0.0	36.850002	9
TCGTTCA	145	0.0	36.41379	16
>>END_MODULE