FastQCFastQC Report
Thu 26 May 2016
SRR1546450_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546450_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1421466
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64140.4512242994204575No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58490.4114766023246423No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC18040.12691123108115143No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGA551.8189894E-1244.0000042
AATGCGC207.8571116E-444.010
GTACGTC207.8571116E-444.012
CGTTTTT72100.042.565881
CGGTCTA3400.040.1176531
GCGCGAC11200.039.4821439
GTTTTTA42150.039.19812
TCACGAC3550.039.0422525
CGACGGT3450.038.89855228
GAGCGAT7200.038.1944477
CGTTGAT1100.038.00000425
CGATGAA8700.037.93103419
CCGTAGA357.2914227E-637.7142872
TCAGACG11500.037.4956522
CTCACGA3700.037.4594624
CGCGACC11800.037.28813610
ACCCGCT10800.037.2777834
GCGTAAG651.0913936E-1137.230771
ACGTGGC11250.037.1555626
CGAATAT1600.037.12514
Produced by FastQC (version 0.11.5)