##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546450_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1421466
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.515153369830866	31.0	31.0	33.0	30.0	34.0
2	31.908159604239568	31.0	31.0	34.0	30.0	34.0
3	32.01023169038162	33.0	31.0	34.0	30.0	34.0
4	35.69847326633208	37.0	35.0	37.0	35.0	37.0
5	35.644073090738715	37.0	35.0	37.0	33.0	37.0
6	35.688557447030036	37.0	35.0	37.0	33.0	37.0
7	36.01221907523641	37.0	35.0	37.0	35.0	37.0
8	18.855540688275344	32.0	0.0	37.0	0.0	37.0
9	28.159252490034937	32.0	17.0	39.0	17.0	39.0
10	33.706334164869226	33.0	32.0	39.0	27.0	39.0
11	35.84301981194063	37.0	35.0	39.0	32.0	39.0
12	36.48129818089212	37.0	35.0	39.0	33.0	39.0
13	36.615762881419606	39.0	35.0	39.0	33.0	39.0
14	37.75582462049743	40.0	36.0	41.0	33.0	41.0
15	38.01321030541708	40.0	36.0	41.0	33.0	41.0
16	38.091935368134024	40.0	36.0	41.0	33.0	41.0
17	38.03994186283738	40.0	36.0	41.0	33.0	41.0
18	38.006499627848996	40.0	36.0	41.0	33.0	41.0
19	37.98731169088814	40.0	36.0	41.0	33.0	41.0
20	37.85272176752733	40.0	36.0	41.0	33.0	41.0
21	37.734726683578785	40.0	36.0	41.0	33.0	41.0
22	37.72815178132998	40.0	36.0	41.0	33.0	41.0
23	37.58641852847694	39.0	35.0	41.0	33.0	41.0
24	37.45068612263677	39.0	35.0	41.0	33.0	41.0
25	37.32671481414258	39.0	35.0	41.0	32.0	41.0
26	37.27995534187944	39.0	35.0	41.0	32.0	41.0
27	37.14006455307408	39.0	35.0	41.0	32.0	41.0
28	37.11927896973969	39.0	35.0	41.0	32.0	41.0
29	36.99859862986522	39.0	35.0	41.0	32.0	41.0
30	36.88885558993321	39.0	35.0	41.0	31.0	41.0
31	36.89638302991419	39.0	35.0	41.0	31.0	41.0
32	36.65159982722063	39.0	35.0	41.0	31.0	41.0
33	36.531410529692586	39.0	35.0	41.0	30.0	41.0
34	36.37913463987179	39.0	35.0	41.0	30.0	41.0
35	36.18116929986366	39.0	35.0	41.0	30.0	41.0
36	36.035693431992044	39.0	35.0	41.0	29.0	41.0
37	35.976665639558036	39.0	35.0	41.0	29.0	41.0
38	35.88848765992292	39.0	35.0	41.0	29.0	41.0
39	35.822472714788816	39.0	35.0	41.0	28.0	41.0
40	35.72146361573193	39.0	35.0	40.0	27.0	41.0
41	35.706577575545246	38.0	35.0	40.0	27.0	41.0
42	35.53206759781803	38.0	35.0	40.0	27.0	41.0
43	35.518907240834466	38.0	35.0	40.0	27.0	41.0
44	35.290053367438965	38.0	34.0	40.0	26.0	41.0
45	35.18907240834462	38.0	34.0	40.0	26.0	41.0
46	35.17747452278141	38.0	34.0	40.0	26.0	41.0
47	35.030874463406086	38.0	34.0	40.0	26.0	41.0
48	35.02900526639399	38.0	34.0	40.0	26.0	41.0
49	34.98858924518771	37.0	34.0	40.0	26.0	41.0
50	34.377475789079725	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	9.0
14	12.0
15	23.0
16	75.0
17	173.0
18	411.0
19	939.0
20	1776.0
21	3195.0
22	5015.0
23	7745.0
24	10882.0
25	15688.0
26	20515.0
27	23130.0
28	24387.0
29	26763.0
30	31970.0
31	40590.0
32	52405.0
33	69290.0
34	105335.0
35	159875.0
36	148047.0
37	222659.0
38	243591.0
39	206960.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.1087060823122	22.527798765499842	28.57247377003741	15.791021382150541
2	33.54494585167707	25.153468320733662	28.223467884564247	13.078117943025017
3	28.45752202303819	25.178653587212075	32.86635065488728	13.497473734862458
4	25.557276783264605	26.56693863940467	33.3107510133904	14.565033563940327
5	23.2996075882223	30.871790109647364	30.882975744759282	14.945626557371053
6	20.214553144429765	40.0926226867192	28.647888869659916	11.044935299191117
7	85.1460393706216	3.679862902102477	9.2435555968275	1.9305421304484243
8	45.09302368118548	49.46646631013334	4.186030478393434	1.2544795302877452
9	82.04684459564984	4.433591798889315	9.592209732768845	3.927353872691995
10	42.99307897621188	27.137546729925305	16.29704825862877	13.572326035234047
11	37.49642974225201	21.797426037625943	24.290274969644017	16.41586925047803
12	34.310282483014014	20.12668611138079	27.49724580116584	18.06578560443936
13	24.304767050355057	28.66920489128829	28.34531392238717	18.680714135969485
14	18.3303716022754	32.63595471154428	29.980105046480183	19.053568639700142
15	17.198371258967853	23.049654370910034	39.792580336075574	19.95939403404654
16	19.778243025158535	20.34906216539826	38.42856600157865	21.44412880786456
17	20.814848895436118	21.452641146534635	29.268234343980087	28.464275614049157
18	22.635996921488097	23.984745326303972	31.242323066467996	22.136934685739934
19	26.70763845213322	25.853942338402746	26.017857620231506	21.420561589232527
20	28.970021090901927	23.797895975000458	26.446218200083578	20.78586473401404
21	24.306736847733255	26.636936796237126	28.5051489096468	20.551177446382816
22	23.893642197562233	21.928276863463495	29.23094889360702	24.947132045367248
23	22.56874241100385	26.917351523005124	27.731722039077965	22.78218402691306
24	22.086845552408573	24.415497802972425	33.14767993043801	20.349976714180993
25	22.260117371783778	24.95015709134091	30.61557575066868	22.174149786206634
26	20.125068063534407	29.288635816825725	27.805308041134996	22.78098807850487
27	21.23779253249814	26.831806036866162	30.065369132993684	21.865032297642014
28	19.702124426472388	25.72956370395071	34.6137719790695	19.954539890507405
29	20.77882974337761	24.26811474913927	33.075641626320994	21.877413881162123
30	22.133065440889897	26.435736064035297	30.72504020497149	20.706158290103318
31	27.241875641063523	24.458974045105546	26.214626308332384	22.084524005498547
32	27.29899976503131	25.855349336530033	26.645871234345385	20.199779664093267
33	26.290182107767613	25.86744952042469	26.15292944045092	21.689438931356783
34	21.816912961688846	25.79843626228133	29.062601567677316	23.322049208352507
35	22.64999655285459	25.198632960619527	29.16038793752366	22.99098254900223
36	26.92635631101975	25.54025210592445	26.16502962434557	21.36836195871023
37	22.47869453085758	28.137781698612557	27.375751512874736	22.007772257655127
38	22.354034496780084	28.389986112928483	25.739553390654436	23.516425999636994
39	22.230781460829878	26.849815612895416	26.202948223875914	24.716454702398792
40	23.829764482583474	23.76750481545109	28.050407114908133	24.352323587057306
41	20.525077631121675	23.865220835391067	28.211789800107777	27.39791173337948
42	22.915426749566997	26.40070181066589	25.317805702000612	25.3660657377665
43	22.51682418010702	26.316000523403304	26.34181893903899	24.825356357450687
44	22.948631905370934	26.44481120195629	28.21024210216776	22.396314790505016
45	21.15372439439283	28.836919068060716	25.865901822484673	24.143454715061775
46	23.866627833518354	27.378424809316577	27.282748936661168	21.4721984205039
47	22.705572978882365	26.33408044933892	27.927646528302468	23.032700043476243
48	22.442745728705436	24.6945758815195	29.33183065933339	23.53084773044167
49	22.388787350524037	23.68716522238309	29.740915364841648	24.183132062251225
50	20.478857742640344	26.534859082102564	29.33084576064429	23.655437414612802
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	548.0
1	1134.5
2	1721.0
3	10719.0
4	19717.0
5	13513.5
6	7310.0
7	7429.5
8	7549.0
9	7750.0
10	7951.0
11	7784.0
12	7617.0
13	7240.5
14	6864.0
15	6614.0
16	6364.0
17	6152.5
18	5941.0
19	5844.5
20	5748.0
21	5852.0
22	5956.0
23	6150.5
24	6345.0
25	7515.0
26	8685.0
27	10945.5
28	13206.0
29	15844.0
30	18482.0
31	21222.5
32	23963.0
33	26442.0
34	28921.0
35	33094.0
36	37267.0
37	42371.5
38	47476.0
39	57283.5
40	67091.0
41	78319.5
42	89548.0
43	96588.0
44	103628.0
45	109846.5
46	116065.0
47	117512.5
48	118960.0
49	116834.5
50	114709.0
51	104700.5
52	94692.0
53	87750.5
54	80809.0
55	77889.5
56	74970.0
57	72650.5
58	70331.0
59	65769.5
60	61208.0
61	56202.0
62	51196.0
63	44154.0
64	37112.0
65	30915.5
66	24719.0
67	21089.0
68	17459.0
69	15429.5
70	13400.0
71	11285.0
72	9170.0
73	6845.5
74	4521.0
75	3293.5
76	2066.0
77	1613.5
78	1161.0
79	877.0
80	593.0
81	422.0
82	251.0
83	176.0
84	101.0
85	70.5
86	40.0
87	27.0
88	14.0
89	10.0
90	6.0
91	7.0
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1421466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.083860373494996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.48538445930404	21.806401851928065
2	6.419435312924773	3.8624279126142405
3	3.0839212607625295	2.7832876983489774
4	2.1619191960483977	2.601555009307942
5	1.664825953216515	2.5042195761368173
6	1.3634573906784901	2.4610837059828907
7	1.220904499468185	2.5710664343960867
8	1.0579658349763206	2.546215716748454
9	0.9517490473373006	2.5769056905632004
>10	9.484117111788885	51.36462226905515
>50	0.07676241104087003	1.4833931126259785
>100	0.02556960353988022	1.6376179349789042
>500	0.0023458346552134527	0.4568110168937352
>1k	0.0011729173276067264	0.4789705502329339
>5k	4.6916693104269056E-4	0.865421520186602
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6414	0.4512242994204575	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5849	0.4114766023246423	No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1804	0.12691123108115143	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026099815261145888	0.0
2	0.0	0.0	0.0	0.09623867190632769	0.0
3	0.0	0.0	0.0	0.1705281730270017	0.0
4	0.0	0.0	0.0	0.2617016516751016	0.0
5	0.0	0.0	0.0	0.4902684974526299	0.0
6	0.0	0.0	0.0	0.7724419718797354	0.0
7	0.0	0.0	0.0	0.9519749329213643	0.0
8	0.0	0.0	0.0	1.3336231749475542	0.0
9	0.0	0.0	0.0	1.663001436545088	0.0
10	0.0	0.0	0.0	2.2144743525346366	0.0
11	0.0	0.0	0.0	2.5940120973698986	0.0
12	0.0	0.0	0.0	2.960535109527769	0.0
13	0.0	0.0	0.0	3.113616505776431	0.0
14	0.0	0.0	0.0	3.1893130050243905	0.0
15	0.0	0.0	0.0	3.2802754339533973	0.0
16	0.0	0.0	0.0	3.4314573827302235	0.0
17	0.0	0.0	0.0	3.5920662189598627	0.0
18	7.034990636427464E-5	0.0	0.0	3.819648166048291	0.0
19	7.034990636427464E-5	0.0	0.0	3.962528825874133	0.0
20	7.034990636427464E-5	0.0	0.0	4.099781493190832	0.0
21	7.034990636427464E-5	0.0	0.0	4.2511744916867515	0.0
22	7.034990636427464E-5	0.0	0.0	4.407280933909077	0.0
23	7.034990636427464E-5	0.0	0.0	4.5803417035651925	0.0
24	7.034990636427464E-5	0.0	0.0	4.719282768634635	0.0
25	7.034990636427464E-5	0.0	0.0	4.835782213573874	0.0
26	7.034990636427464E-5	0.0	0.0	4.9476385646930705	0.0
27	7.034990636427464E-5	0.0	0.0	5.073002097834208	0.0
28	7.034990636427464E-5	0.0	0.0	5.19498883546986	0.0
29	7.034990636427464E-5	0.0	0.0	5.329919955876539	0.0
30	7.034990636427464E-5	0.0	0.0	5.503965624221754	0.0
31	7.034990636427464E-5	0.0	0.0	5.644524737137575	0.0
32	7.034990636427464E-5	0.0	0.0	5.7900083434988945	0.0
33	7.034990636427464E-5	0.0	0.0	5.9295122078192515	0.0
34	7.034990636427464E-5	0.0	0.0	6.0768249117460424	0.0
35	7.034990636427464E-5	0.0	0.0	6.252488627937637	0.0
36	7.034990636427464E-5	0.0	0.0	6.4019821789617195	0.0
37	7.034990636427464E-5	0.0	0.0	6.563998013318645	0.0
38	1.4069981272854927E-4	0.0	0.0	6.7381843814765885	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGA	55	1.8189894E-12	44.000004	2
AATGCGC	20	7.8571116E-4	44.0	10
GTACGTC	20	7.8571116E-4	44.0	12
CGTTTTT	7210	0.0	42.56588	1
CGGTCTA	340	0.0	40.11765	31
GCGCGAC	1120	0.0	39.482143	9
GTTTTTA	4215	0.0	39.1981	2
TCACGAC	355	0.0	39.04225	25
CGACGGT	345	0.0	38.898552	28
GAGCGAT	720	0.0	38.194447	7
CGTTGAT	110	0.0	38.000004	25
CGATGAA	870	0.0	37.931034	19
CCGTAGA	35	7.2914227E-6	37.714287	2
TCAGACG	1150	0.0	37.49565	22
CTCACGA	370	0.0	37.45946	24
CGCGACC	1180	0.0	37.288136	10
ACCCGCT	1080	0.0	37.27778	34
GCGTAAG	65	1.0913936E-11	37.23077	1
ACGTGGC	1125	0.0	37.15556	26
CGAATAT	160	0.0	37.125	14
>>END_MODULE