FastQCFastQC Report
Thu 26 May 2016
SRR1546449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546449_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1664725
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107840.6477946808031356No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98550.5919896679631771No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT254.4437023E-544.021
CGCGCGA207.857501E-444.02
CGTTTTT146950.043.1915661
TCGCTAT850.041.41176623
GTTACGA750.041.0666662
GAGCGAT10200.040.5490157
GTTTTTA84750.039.9764022
ACGCGAG1050.039.8095253
CGATGAA9050.039.6243119
GTAAGCG1000.039.6000021
TATAGCG950.039.368421
TCGCGGA452.3519533E-839.111112
TTACGAG3400.038.176473
CGTATGA750.038.133332
TATACGA750.038.133332
TATCTCG357.292174E-637.7142871
CTCGACG357.292174E-637.7142871
CGCGTAA357.292174E-637.71428731
TTCGCGA700.037.7142872
GCGTAAC357.292174E-637.71428732
Produced by FastQC (version 0.11.5)