##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546449_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1664725
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.568761206805927	31.0	31.0	34.0	30.0	34.0
2	31.94460947003259	31.0	31.0	34.0	30.0	34.0
3	32.00999204072745	33.0	31.0	34.0	30.0	34.0
4	35.72179068614936	37.0	35.0	37.0	35.0	37.0
5	35.66225652885612	37.0	35.0	37.0	33.0	37.0
6	35.71061226328673	37.0	35.0	37.0	33.0	37.0
7	36.07526708615537	37.0	35.0	37.0	35.0	37.0
8	18.132505969454414	18.0	0.0	37.0	0.0	37.0
9	27.76984907417141	19.0	17.0	39.0	17.0	39.0
10	33.57577677994864	32.0	32.0	39.0	27.0	39.0
11	35.76380002703149	37.0	35.0	39.0	32.0	39.0
12	36.59425250416736	37.0	35.0	39.0	33.0	39.0
13	36.82180960819355	39.0	35.0	39.0	33.0	39.0
14	38.029146555737434	40.0	37.0	41.0	33.0	41.0
15	38.24568442235204	40.0	37.0	41.0	33.0	41.0
16	38.314199041883796	40.0	37.0	41.0	34.0	41.0
17	38.25812371412696	40.0	37.0	41.0	33.0	41.0
18	38.18642358347475	40.0	37.0	41.0	33.0	41.0
19	38.1758026100407	40.0	37.0	41.0	34.0	41.0
20	38.008197149679376	40.0	36.0	41.0	34.0	41.0
21	37.91768370151226	40.0	36.0	41.0	33.0	41.0
22	37.908616738500356	40.0	36.0	41.0	33.0	41.0
23	37.71633513042695	39.0	36.0	41.0	33.0	41.0
24	37.53930769346288	39.0	35.0	41.0	33.0	41.0
25	37.4396972472931	39.0	35.0	41.0	33.0	41.0
26	37.3820396762228	39.0	35.0	41.0	33.0	41.0
27	37.230341347670034	39.0	35.0	41.0	32.0	41.0
28	37.2069482947634	39.0	35.0	41.0	33.0	41.0
29	37.110009160672185	39.0	35.0	41.0	32.0	41.0
30	36.99633933532566	39.0	35.0	41.0	32.0	41.0
31	36.92548198651429	39.0	35.0	41.0	31.0	41.0
32	36.56062713060716	39.0	35.0	41.0	30.0	41.0
33	36.32155040622325	39.0	35.0	41.0	30.0	41.0
34	36.07375332262085	39.0	35.0	41.0	28.0	41.0
35	35.82290468395681	39.0	35.0	41.0	26.0	41.0
36	35.67918064545195	39.0	35.0	41.0	25.0	41.0
37	35.61299854330295	39.0	35.0	41.0	25.0	41.0
38	35.538180179909595	39.0	35.0	41.0	24.0	41.0
39	35.45640811545451	39.0	35.0	41.0	24.0	41.0
40	35.41617264112691	39.0	35.0	41.0	24.0	41.0
41	35.37748757302257	39.0	35.0	41.0	23.0	41.0
42	35.21816996801274	39.0	35.0	41.0	23.0	41.0
43	35.23922689933773	39.0	35.0	40.0	23.0	41.0
44	35.02337082701347	39.0	35.0	40.0	23.0	41.0
45	34.95070837525717	38.0	35.0	40.0	23.0	41.0
46	34.95827418943069	38.0	35.0	40.0	23.0	41.0
47	34.82710657916473	38.0	34.0	40.0	22.0	41.0
48	34.836397603207736	38.0	34.0	40.0	23.0	41.0
49	34.8065632461818	38.0	34.0	40.0	22.0	41.0
50	34.204054723753174	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	3.0
14	5.0
15	26.0
16	66.0
17	171.0
18	477.0
19	1107.0
20	2279.0
21	4126.0
22	6720.0
23	10643.0
24	15759.0
25	23563.0
26	31337.0
27	34156.0
28	32711.0
29	32293.0
30	36064.0
31	44338.0
32	57183.0
33	75095.0
34	110314.0
35	163541.0
36	164364.0
37	256635.0
38	291849.0
39	269894.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.271170914114943	22.7686855186292	27.83552839057502	18.124615176680834
2	35.36632176485605	24.443977233477003	27.950322125275946	12.239378876390996
3	26.46166784303714	24.664974695520282	36.09232756160928	12.781029899833307
4	23.95218429470333	25.676973674330593	36.59937827569118	13.771463755274896
5	22.12119118773371	30.352580756581414	34.192314045863434	13.333914009821443
6	18.875309735842254	39.66066467434561	31.020138461307422	10.44388712850471
7	82.57141569928967	2.902941927345357	12.782711859316104	1.742930514048867
8	41.814113442160114	51.17758188289358	5.759569898932257	1.2487347760140564
9	78.36753818198201	4.618600669780294	13.304179368964844	3.7096817792728527
10	37.409121626695104	33.250717085404496	19.008605024853956	10.331556263046448
11	29.21773866554536	23.001877186922766	33.369655648830886	14.410728498700987
12	27.09816936731292	21.93335235549415	33.270239829401255	17.698238447791677
13	21.029599483398158	28.085179233807384	34.74207451681209	16.14314676598237
14	16.341257565063298	31.891513613359564	34.53537371037258	17.231855111204553
15	15.746024118097585	25.37782516631876	42.49386535313641	16.382285362447252
16	18.710057216657404	23.60858400036042	40.088723362717566	17.592635420264607
17	18.40382045082521	24.53131898661941	34.18300319872651	22.88185736382886
18	19.93158029103906	24.561834537235878	36.59060805838802	18.91597711333704
19	22.676478096982986	26.819504723002296	31.789514784724204	18.714502395290513
20	24.755620297646757	25.575034915676763	31.631831083211942	18.037513703464537
21	21.562540359518838	27.49427082551172	33.097838982414515	17.845349832554927
22	20.55630809893526	23.572001381609574	33.672288215771374	22.19940230368379
23	18.505398789589872	27.559867245340826	33.31415098589857	20.620582979170734
24	18.37072189100302	25.684242142095542	37.83165387676644	18.11338209013501
25	18.251002417816757	27.33232215531094	35.22461667843037	19.192058748441934
26	18.11284146030125	29.966871405187046	33.2011593506435	18.719127783868206
27	19.04116295484239	27.76698854165102	33.84667137214855	19.34517713135803
28	17.115619696946943	27.352505669104506	37.3525957740768	18.17927885987175
29	17.996726186006697	24.99037378546006	36.79310396612053	20.219796062412712
30	19.569718722311492	27.37503191217769	33.64753938338164	19.40770998212918
31	22.860292240460137	25.62663502980973	30.280376638784183	21.23269609094595
32	23.057622129781194	27.452582258330953	30.314736668218472	19.175058943669374
33	22.26475844358678	26.743275916442656	29.928907176861042	21.06305846310952
34	18.61905119464176	27.86207932241061	31.118833440958717	22.400036041988916
35	19.28865127873973	26.423102914895853	32.317469852378025	21.970775953986394
36	22.29905840303954	27.291114147982398	30.02718166664164	20.38264578233642
37	19.586658457102523	28.791422006637735	31.42164621784379	20.200273318415952
38	19.5283905750199	29.219540764991216	30.306266800822957	20.94580185916593
39	20.53444262565889	28.47677544339155	30.308669600084098	20.68011233086546
40	22.03703314361231	25.63822853624472	30.503836970070132	21.820901350072834
41	19.283785610235928	25.719323011308177	30.49446605295169	24.502425325504213
42	20.799951944014776	27.188394479568696	28.95679466578564	23.054858910630884
43	20.694408986469238	27.204433164636804	29.934253405217078	22.166904443676884
44	20.69555031611828	28.694589196413823	30.34062683025725	20.26923365721065
45	19.189235459310098	30.08917388757903	28.848428419108263	21.873162234002614
46	21.316794065085826	28.875459910796074	28.99632071363138	20.811425310486715
47	20.25175329258586	26.99779242817883	30.37630839928517	22.37414587995014
48	20.78445388878043	25.17544939854931	31.703734851101533	22.336361861568726
49	20.44668038264578	24.979561188784935	32.31728964243344	22.256468786135848
50	19.22665905780234	27.90358767964679	31.092222439141597	21.777530823409272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	411.0
1	924.0
2	1437.0
3	17998.0
4	34559.0
5	24642.0
6	14725.0
7	14705.5
8	14686.0
9	15079.0
10	15472.0
11	15414.5
12	15357.0
13	14684.0
14	14011.0
15	13287.5
16	12564.0
17	11980.5
18	11397.0
19	11065.5
20	10734.0
21	11027.5
22	11321.0
23	12538.0
24	13755.0
25	15845.5
26	17936.0
27	21077.0
28	24218.0
29	28094.0
30	31970.0
31	37615.0
32	43260.0
33	49030.0
34	54800.0
35	60746.5
36	66693.0
37	73756.5
38	80820.0
39	90704.0
40	100588.0
41	111999.0
42	123410.0
43	131629.0
44	139848.0
45	146319.0
46	152790.0
47	149690.0
48	146590.0
49	137518.5
50	128447.0
51	114184.5
52	99922.0
53	88641.0
54	77360.0
55	68773.5
56	60187.0
57	53550.0
58	46913.0
59	41889.5
60	36866.0
61	31938.0
62	27010.0
63	21267.5
64	15525.0
65	11805.0
66	8085.0
67	6145.0
68	4205.0
69	3522.5
70	2840.0
71	2385.5
72	1931.0
73	1511.0
74	1091.0
75	810.0
76	529.0
77	389.5
78	250.0
79	174.0
80	98.0
81	85.0
82	72.0
83	47.5
84	23.0
85	17.0
86	11.0
87	8.0
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1664725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.14370393544599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.85827481834156	25.972242103460513
2	7.69357831869885	5.561488339104352
3	4.024945837775345	4.36429352150273
4	2.6367835188751436	3.812124913923466
5	2.0225579910470763	3.6551368610337978
6	1.5933956700134613	3.4554732809392883
7	1.3405498727369913	3.3916706397633924
8	1.114161751105711	3.22159459745303
9	0.9720586407453132	3.1620419747091586
>10	6.684111419382272	39.379103857111346
>50	0.04412694064419596	1.0459742223800867
>100	0.013024099339801849	0.9565966560563993
>500	0.0010419091261315856	0.23417282589975677
>1k	0.0010419091261315856	0.492698119354889
>5k	1.7365152102193096E-4	0.6185401230882077
>10k+	1.7365152102193096E-4	0.6768479642195061
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10784	0.6477946808031356	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9855	0.5919896679631771	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.003499076424034E-4	0.0	0.0	0.026070371983360614	0.0
2	3.003499076424034E-4	0.0	0.0	0.10638393728693929	0.0
3	3.003499076424034E-4	0.0	0.0	0.1853158930153629	0.0
4	3.003499076424034E-4	0.0	0.0	0.31350523359714066	0.0
5	3.003499076424034E-4	0.0	0.0	0.5988977158389523	0.0
6	3.003499076424034E-4	0.0	0.0	1.0301401132319152	0.0
7	3.003499076424034E-4	0.0	0.0	1.3427442971061287	0.0
8	3.6041988917088406E-4	0.0	0.0	2.0172701196894383	0.0
9	3.6041988917088406E-4	0.0	0.0	2.7725900674285544	0.0
10	3.6041988917088406E-4	0.0	0.0	3.654597606211236	0.0
11	3.6041988917088406E-4	0.0	0.0	4.071843697908063	0.0
12	3.6041988917088406E-4	0.0	0.0	4.528495697487573	0.0
13	3.6041988917088406E-4	0.0	0.0	4.7652314946913155	0.0
14	3.6041988917088406E-4	0.0	0.0	4.8823679586718525	0.0
15	3.6041988917088406E-4	0.0	0.0	4.991575185090631	0.0
16	3.6041988917088406E-4	0.0	0.0	5.149859586418177	0.0
17	3.6041988917088406E-4	0.0	0.0	5.332532400246287	0.0
18	3.6041988917088406E-4	0.0	0.0	5.60639144603463	0.0
19	3.6041988917088406E-4	0.0	0.0	5.78155551217168	0.0
20	3.6041988917088406E-4	0.0	0.0	5.951733769841866	0.0
21	3.6041988917088406E-4	0.0	0.0	6.122993287179564	0.0
22	3.6041988917088406E-4	0.0	0.0	6.31137274925288	0.0
23	3.6041988917088406E-4	0.0	0.0	6.519094745378366	0.0
24	3.6041988917088406E-4	0.0	0.0	6.685308384267672	0.0
25	8.409797413987296E-4	0.0	0.0	6.827313820601	0.0
26	8.409797413987296E-4	0.0	0.0	6.962651488984667	0.0
27	8.409797413987296E-4	0.0	0.0	7.111745183138356	0.0
28	9.010497229272102E-4	0.0	0.0	7.270329934373545	0.0
29	9.010497229272102E-4	0.0	0.0	7.436002943429095	0.0
30	9.010497229272102E-4	0.0	0.0	7.651774317079398	0.0
31	9.010497229272102E-4	0.0	0.0	7.833846431092223	0.0
32	9.010497229272102E-4	0.0	0.0	8.014116445659193	0.0
33	9.010497229272102E-4	0.0	0.0	8.190782261334455	0.0
34	9.010497229272102E-4	0.0	0.0	8.373334935199507	0.0
35	9.010497229272102E-4	0.0	0.0	8.578594062082326	0.0
36	9.010497229272102E-4	0.0	0.0	8.768775623601496	0.0
37	9.010497229272102E-4	0.0	0.0	8.974875730225714	0.0
38	9.010497229272102E-4	0.0	0.0	9.191608223580472	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGT	25	4.4437023E-5	44.0	21
CGCGCGA	20	7.857501E-4	44.0	2
CGTTTTT	14695	0.0	43.191566	1
TCGCTAT	85	0.0	41.411766	23
GTTACGA	75	0.0	41.066666	2
GAGCGAT	1020	0.0	40.549015	7
GTTTTTA	8475	0.0	39.976402	2
ACGCGAG	105	0.0	39.809525	3
CGATGAA	905	0.0	39.62431	19
GTAAGCG	100	0.0	39.600002	1
TATAGCG	95	0.0	39.36842	1
TCGCGGA	45	2.3519533E-8	39.11111	2
TTACGAG	340	0.0	38.17647	3
CGTATGA	75	0.0	38.13333	2
TATACGA	75	0.0	38.13333	2
TATCTCG	35	7.292174E-6	37.714287	1
CTCGACG	35	7.292174E-6	37.714287	1
CGCGTAA	35	7.292174E-6	37.714287	31
TTCGCGA	70	0.0	37.714287	2
GCGTAAC	35	7.292174E-6	37.714287	32
>>END_MODULE