FastQCFastQC Report
Thu 26 May 2016
SRR1546448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546448_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences352419
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15850.44974873658911696No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.4471949582740999No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCC4150.11775755563689812No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAC254.436539E-544.09
TTGTGCG207.8490435E-444.01
CGACGTC207.8490435E-444.018
ACGTTAG207.8490435E-444.01
CGCGCGA207.8490435E-444.02
TCGATAG254.436539E-544.01
GTTACGA254.436539E-544.02
CGAGGTA254.436539E-544.06
AGCGATA302.5227018E-644.08
GGACGAT207.8490435E-444.06
TATAGCG254.436539E-544.01
TTTTGCG207.8490435E-444.01
GACGATT207.8490435E-444.07
AATAGCG207.8490435E-444.01
CACAACG207.8490435E-444.012
TTCGCGA207.8490435E-444.02
CTTACGA207.8490435E-444.02
CGACGGT351.4422585E-743.99999628
ACTAACC351.4422585E-743.99999614
CGGTCTA351.4422585E-743.99999631
Produced by FastQC (version 0.11.5)