FastQCFastQC Report
Thu 26 May 2016
SRR1546447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546447_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1263350
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93660.7413622511576364No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89600.7092254719594728No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCC12930.10234693473700875No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGTA207.856778E-444.0000045
GCGGAAC207.856778E-444.0000044
CTACGCG254.4430926E-544.01
AATCGCG254.4430926E-544.01
CGTTTTT124850.043.1365621
CGACGGT1650.041.33333228
CGGTCTA1650.041.33333231
GTTACGA750.041.0666662
CGTTGGA603.6379788E-1240.3333322
GAGCGAT6400.040.2187547
TACGGGA2200.040.04
GTTTTTA69450.039.565152
CGGGTAT452.3512257E-839.111116
ATTAGCG900.039.111111
GGTCGAA404.1249768E-738.50000442
TGTACGA1000.037.3999982
TGTGCGA1300.037.230772
CGGAGAT1300.037.230775
CGCGACC2250.037.15555610
CGCGCGA301.3011685E-436.6666642
Produced by FastQC (version 0.11.5)