##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546447_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1263350
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.621021094708514	31.0	31.0	34.0	30.0	34.0
2	32.00034194799541	31.0	31.0	34.0	30.0	34.0
3	32.06699331143388	33.0	31.0	34.0	30.0	34.0
4	35.77074761546682	37.0	35.0	37.0	35.0	37.0
5	35.71879764119207	37.0	35.0	37.0	33.0	37.0
6	35.771235999525075	37.0	35.0	37.0	35.0	37.0
7	36.09341037717181	37.0	35.0	37.0	35.0	37.0
8	18.14058257806625	18.0	0.0	37.0	0.0	37.0
9	27.790171369770846	19.0	17.0	39.0	17.0	39.0
10	33.66709621245102	32.0	32.0	39.0	27.0	39.0
11	35.84823999683381	37.0	35.0	39.0	32.0	39.0
12	36.65795701903669	38.0	35.0	39.0	33.0	39.0
13	36.883153520402104	39.0	35.0	39.0	33.0	39.0
14	38.10848616772866	40.0	37.0	41.0	33.0	41.0
15	38.328885107056635	40.0	37.0	41.0	33.0	41.0
16	38.38903550085091	40.0	37.0	41.0	34.0	41.0
17	38.33026081450113	40.0	37.0	41.0	34.0	41.0
18	38.27158111370562	40.0	37.0	41.0	34.0	41.0
19	38.23878339335893	40.0	37.0	41.0	34.0	41.0
20	38.05450191949974	40.0	36.0	41.0	34.0	41.0
21	37.965961926623656	40.0	36.0	41.0	33.0	41.0
22	37.96715716151502	40.0	36.0	41.0	33.0	41.0
23	37.77534808247912	39.0	36.0	41.0	33.0	41.0
24	37.573914592155774	39.0	35.0	41.0	33.0	41.0
25	37.47855621957494	39.0	35.0	41.0	33.0	41.0
26	37.41230775319587	39.0	35.0	41.0	33.0	41.0
27	37.25240273875015	39.0	35.0	41.0	33.0	41.0
28	37.23214232002216	39.0	35.0	41.0	33.0	41.0
29	37.10947164285432	39.0	35.0	41.0	32.0	41.0
30	36.98104167491194	39.0	35.0	41.0	32.0	41.0
31	36.85939605018403	39.0	35.0	41.0	31.0	41.0
32	36.459253571852614	39.0	35.0	41.0	30.0	41.0
33	36.170047888550286	39.0	35.0	41.0	29.0	41.0
34	35.89196026437646	39.0	35.0	41.0	26.0	41.0
35	35.62410812522262	39.0	35.0	41.0	24.0	41.0
36	35.474904816559146	39.0	35.0	41.0	23.0	41.0
37	35.359411089563466	39.0	35.0	41.0	23.0	41.0
38	35.281466735267344	39.0	35.0	41.0	23.0	41.0
39	35.19761823722642	39.0	35.0	41.0	23.0	41.0
40	35.139517948312026	39.0	35.0	41.0	22.0	41.0
41	35.14090948668223	39.0	35.0	41.0	22.0	41.0
42	34.95188665057189	39.0	35.0	41.0	21.0	41.0
43	34.9533169747101	39.0	35.0	41.0	21.0	41.0
44	34.75556813234654	38.0	34.0	40.0	20.0	41.0
45	34.70126409941821	38.0	34.0	40.0	20.0	41.0
46	34.7167356631179	38.0	34.0	40.0	20.0	41.0
47	34.5914718803182	38.0	34.0	40.0	20.0	41.0
48	34.59902956425377	38.0	34.0	40.0	20.0	41.0
49	34.57191039696046	38.0	34.0	40.0	20.0	41.0
50	33.96321051173467	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	2.0
13	3.0
14	7.0
15	16.0
16	31.0
17	117.0
18	329.0
19	842.0
20	1797.0
21	3258.0
22	5508.0
23	8560.0
24	12828.0
25	19489.0
26	26023.0
27	27805.0
28	26326.0
29	25054.0
30	27568.0
31	32683.0
32	41884.0
33	55015.0
34	81888.0
35	124714.0
36	121118.0
37	190991.0
38	223368.0
39	206124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.13535441484941	22.59263070408042	25.95155736731705	19.320457513753116
2	35.99627973245735	24.3429770055804	27.955831717259667	11.704911544702576
3	26.45838445403095	24.430047097004	37.289587208612026	11.821981240353029
4	23.2372659991293	25.733802984129493	37.6806902283611	13.3482407883801
5	22.02936636719832	29.883009458978115	35.242569359243284	12.845054814580282
6	18.566667985910478	39.17378398701864	32.145565361934544	10.113982665136344
7	81.91253413543357	2.970039973087426	13.644041635334625	1.4733842561443782
8	41.5811928602525	51.26196224324217	6.141211857363359	1.0156330391419637
9	78.13424625004947	4.57347528396723	13.93730953417501	3.3549689318082874
10	38.881545098349626	32.22487829975858	19.469901452487434	9.423675149404362
11	29.197451220960147	22.484109708315195	34.00601575177109	14.312423318953577
12	26.36165749792219	21.838287093837813	34.24189654490046	17.558158863339536
13	20.611944433450745	28.580994973681083	34.9390113586892	15.868049234178969
14	16.182451418846718	31.72652075830134	35.62781493647841	16.46321288637353
15	15.94601654331737	24.595955198480233	43.46523132940199	15.992796928800413
16	18.42466458226145	23.491906439229034	40.82154588989591	17.261883088613605
17	18.47936043060118	23.908813867890924	34.83207345549531	22.779752246012585
18	20.199311354731467	24.005461669371115	37.18264930541814	18.61257767047928
19	22.921676495033047	25.74314323030039	32.74682392052875	18.588356354137808
20	24.787192781097875	25.175446234218548	32.6971939684173	17.340167016266275
21	21.308188546325248	26.743024498357542	34.51315945699925	17.435627498317967
22	20.378438279178372	23.37182886769304	35.09827047136581	21.151462381762773
23	18.5197292911703	27.321723987810188	34.08105433965251	20.077492381367
24	18.206118652788223	25.21898127993034	38.52147069299877	18.05342937428266
25	17.848418886294375	27.328214667352675	36.22036648593026	18.602999960422686
26	17.671033363675942	30.019788657141728	33.915462856690546	18.393715122491788
27	18.5194918272846	27.688130763446395	35.25634226461392	18.536035144655084
28	16.913365259033522	26.737404519729292	38.96766533423042	17.381564887006768
29	17.350932045751374	24.805556654925397	38.61542723710769	19.22808406221554
30	19.421537974433058	26.78276012189813	35.50259231408557	18.29310958958325
31	23.232041793643884	25.17639608976135	31.86013377132228	19.73142834527249
32	23.85831321486524	27.45304151660268	31.603039537736972	17.08560573079511
33	23.166264297304785	27.054418807139747	31.049352910911466	18.729963984644
34	19.37483674357858	27.746863497843037	32.31820160683896	20.56009815173942
35	20.247199905014448	27.408635769976648	32.680254877903984	19.663909447104917
36	23.39842482289152	27.34831994300867	30.840305536866268	18.412949697233547
37	20.52764475402699	29.12494558119286	31.100486800965687	19.24692286381446
38	20.605770372422526	29.76570229944196	29.17109272964737	20.45743459848815
39	20.91209878497645	28.949143151145762	29.737127478529306	20.401630585348478
40	22.278228519412675	26.514505085684885	29.682985712589545	21.5242806823129
41	19.47750029682986	25.761982031899315	30.066173269481933	24.694344401788896
42	21.53393754699806	27.29971900106859	28.668698302133215	22.497645149800135
43	21.341354335694778	27.40808168757668	29.460956979459375	21.789606997269164
44	20.561918708196462	28.860015039379427	30.6517592116199	19.92630704080421
45	19.615387659793406	29.974512209601457	29.105077769422564	21.305022361182573
46	21.596944631337315	29.193335180274666	29.398583132148655	19.811137056239364
47	20.850833102465664	27.057426683025287	30.414216171290615	21.677524043218426
48	21.314995844382	25.519373095341752	31.40839830609095	21.757232754185303
49	20.79882851149721	24.919697629318875	32.55234099813987	21.729132861044047
50	19.625677761507106	28.164087545019196	31.222543238215856	20.987691455257846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	261.0
1	716.5
2	1172.0
3	15805.5
4	30439.0
5	21534.5
6	12630.0
7	12799.5
8	12969.0
9	12990.5
10	13012.0
11	12954.0
12	12896.0
13	12527.5
14	12159.0
15	11442.0
16	10725.0
17	10163.0
18	9601.0
19	9255.5
20	8910.0
21	8850.0
22	8790.0
23	9781.5
24	10773.0
25	12712.0
26	14651.0
27	16779.5
28	18908.0
29	22196.0
30	25484.0
31	29210.0
32	32936.0
33	36696.0
34	40456.0
35	45140.5
36	49825.0
37	54474.0
38	59123.0
39	67925.5
40	76728.0
41	86178.0
42	95628.0
43	100401.5
44	105175.0
45	107683.5
46	110192.0
47	108277.5
48	106363.0
49	100472.0
50	94581.0
51	84890.0
52	75199.0
53	67186.0
54	59173.0
55	52262.0
56	45351.0
57	40711.0
58	36071.0
59	31790.0
60	27509.0
61	23448.0
62	19387.0
63	15745.0
64	12103.0
65	9130.5
66	6158.0
67	4792.0
68	3426.0
69	2721.0
70	2016.0
71	1659.0
72	1302.0
73	991.0
74	680.0
75	493.0
76	306.0
77	235.5
78	165.0
79	115.0
80	65.0
81	46.0
82	27.0
83	21.5
84	16.0
85	10.5
86	5.0
87	3.0
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1263350.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.75602767278758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.77191391392765	26.343103125176054
2	8.086263107567843	6.106103473175457
3	4.479487835230336	5.073830000006159
4	3.143150776743764	4.746915508259254
5	2.4844796248029026	4.690204073326766
6	1.9349716992964572	4.383410701481871
7	1.6279731432454612	4.302605933285144
8	1.3521669962558398	4.084196362309244
9	1.1743977237084207	3.9906533659674497
>10	5.905073286252733	32.769197142823415
>50	0.024716484029900048	0.6108691636643167
>100	0.013717146274507371	1.0134200730898915
>500	0.0010551641651057803	0.3222959520040773
>1k	2.1103283302115604E-4	0.10302315598053695
>5k	4.220656660423121E-4	1.4601719694503636
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9366	0.7413622511576364	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8960	0.7092254719594728	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCC	1293	0.10234693473700875	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03063284125539241	0.0
2	0.0	0.0	0.0	0.11255788182213955	0.0
3	0.0	0.0	0.0	0.19005026318913998	0.0
4	0.0	0.0	0.0	0.28274033324098624	0.0
5	0.0	0.0	0.0	0.5111014366565085	0.0
6	0.0	0.0	0.0	0.8336565480666482	0.0
7	0.0	0.0	0.0	1.0768195670241818	0.0
8	0.0	0.0	0.0	1.5581588633395338	0.0
9	0.0	0.0	0.0	2.054141765939763	0.0
10	0.0	0.0	0.0	2.7382752206435272	0.0
11	0.0	0.0	0.0	3.09067162702339	0.0
12	0.0	0.0	0.0	3.514544662999169	0.0
13	0.0	0.0	0.0	3.719475996358887	0.0
14	0.0	0.0	0.0	3.8421656706375904	0.0
15	0.0	0.0	0.0	3.944195986860332	0.0
16	0.0	0.0	0.0	4.093639925594649	0.0
17	0.0	0.0	0.0	4.2647722325562984	0.0
18	0.0	0.0	0.0	4.541021886254799	0.0
19	0.0	0.0	0.0	4.710412791387976	0.0
20	0.0	0.0	0.0	4.861598131950766	0.0
21	0.0	0.0	0.0	5.018957533541774	0.0
22	0.0	0.0	0.0	5.195076582103138	0.0
23	0.0	0.0	0.0	5.384968536035145	0.0
24	0.0	0.0	0.0	5.530296434083983	0.0
25	0.0	0.0	0.0	5.670083508133138	0.0
26	0.0	0.0	0.0	5.798393161040091	0.0
27	0.0	0.0	0.0	5.9551984802311315	0.0
28	0.0	0.0	0.0	6.095302172794554	0.0
29	0.0	0.0	0.0	6.251474254957059	0.0
30	0.0	0.0	0.0	6.459809237345154	0.0
31	0.0	0.0	0.0	6.63078323504967	0.0
32	0.0	0.0	0.0	6.796058099497368	0.0
33	0.0	0.0	0.0	6.956979459373887	0.0
34	0.0	0.0	0.0	7.124470653421459	0.0
35	0.0	0.0	0.0	7.312858669410693	0.0
36	0.0	0.0	0.0	7.4930146040289705	0.0
37	0.0	0.0	0.0	7.678948826532632	0.0
38	0.0	0.0	0.0	7.869394862864606	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAGTA	20	7.856778E-4	44.000004	5
GCGGAAC	20	7.856778E-4	44.000004	4
CTACGCG	25	4.4430926E-5	44.0	1
AATCGCG	25	4.4430926E-5	44.0	1
CGTTTTT	12485	0.0	43.136562	1
CGACGGT	165	0.0	41.333332	28
CGGTCTA	165	0.0	41.333332	31
GTTACGA	75	0.0	41.066666	2
CGTTGGA	60	3.6379788E-12	40.333332	2
GAGCGAT	640	0.0	40.218754	7
TACGGGA	220	0.0	40.0	4
GTTTTTA	6945	0.0	39.56515	2
CGGGTAT	45	2.3512257E-8	39.11111	6
ATTAGCG	90	0.0	39.11111	1
GGTCGAA	40	4.1249768E-7	38.500004	42
TGTACGA	100	0.0	37.399998	2
TGTGCGA	130	0.0	37.23077	2
CGGAGAT	130	0.0	37.23077	5
CGCGACC	225	0.0	37.155556	10
CGCGCGA	30	1.3011685E-4	36.666664	2
>>END_MODULE