FastQCFastQC Report
Thu 26 May 2016
SRR1546446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546446_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1041769
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61300.58842219340372No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57990.5566493147713168No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC207.856141E-444.0000046
TCGCAAG207.856141E-444.0000041
CGCATGA302.5274876E-644.0000042
CGTCGAG207.856141E-444.0000043
TCGACTC207.856141E-444.00000420
ATCGATC207.856141E-444.00000443
TTCGGAT254.4425527E-544.015
TATCGGT254.4425527E-544.040
TCTATCG254.4425527E-544.038
TCGCGGA454.802132E-1043.9999962
TAGTACG454.802132E-1043.9999961
CGTTTTT73600.043.2228241
TATGCGA1000.041.82
ACGGGAC1900.041.684215
CACGACG1650.041.33333226
CGACGGT1600.041.25000428
TGTTACG700.040.8571431
GCGTAAG700.040.8571431
CGTAAGA650.040.6153832
AACGTTG557.8216544E-1140.01
Produced by FastQC (version 0.11.5)