##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546446_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1041769
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.599598375455596	31.0	31.0	34.0	30.0	34.0
2	31.97417565698346	31.0	31.0	34.0	30.0	34.0
3	32.0535809762049	33.0	31.0	34.0	30.0	34.0
4	35.75072784849616	37.0	35.0	37.0	35.0	37.0
5	35.68970472340797	37.0	35.0	37.0	33.0	37.0
6	35.74821385547084	37.0	35.0	37.0	35.0	37.0
7	36.091992562650645	37.0	35.0	37.0	35.0	37.0
8	18.022838076387377	0.0	0.0	37.0	0.0	37.0
9	27.735816673370007	19.0	17.0	39.0	17.0	39.0
10	33.65177404971735	32.0	32.0	39.0	27.0	39.0
11	35.81587568837237	37.0	35.0	39.0	32.0	39.0
12	36.50651823964814	37.0	35.0	39.0	33.0	39.0
13	36.64251383944041	39.0	35.0	39.0	33.0	39.0
14	37.820672337149595	40.0	36.0	41.0	33.0	41.0
15	38.10061539554354	40.0	37.0	41.0	33.0	41.0
16	38.20575386674013	40.0	37.0	41.0	34.0	41.0
17	38.169782360580896	40.0	36.0	41.0	34.0	41.0
18	38.12589547202883	40.0	36.0	41.0	33.0	41.0
19	38.11347525219122	40.0	37.0	41.0	34.0	41.0
20	37.964346222627086	40.0	36.0	41.0	34.0	41.0
21	37.83992324594032	40.0	36.0	41.0	33.0	41.0
22	37.85501488333786	40.0	36.0	41.0	33.0	41.0
23	37.70954981382629	39.0	35.0	41.0	33.0	41.0
24	37.56248746123181	39.0	35.0	41.0	33.0	41.0
25	37.44831627740891	39.0	35.0	41.0	33.0	41.0
26	37.390168069888816	39.0	35.0	41.0	33.0	41.0
27	37.21413672320831	39.0	35.0	41.0	33.0	41.0
28	37.23186810127773	39.0	35.0	41.0	33.0	41.0
29	37.137186842764564	39.0	35.0	41.0	32.0	41.0
30	37.04122986957761	39.0	35.0	41.0	32.0	41.0
31	36.951482526356614	39.0	35.0	41.0	32.0	41.0
32	36.65437443425558	39.0	35.0	41.0	31.0	41.0
33	36.459387829739605	39.0	35.0	41.0	30.0	41.0
34	36.308441698687524	39.0	35.0	41.0	30.0	41.0
35	36.104229440499765	39.0	35.0	41.0	29.0	41.0
36	35.96312426267244	39.0	35.0	41.0	29.0	41.0
37	35.87643037947952	39.0	35.0	41.0	27.0	41.0
38	35.82710082561489	39.0	35.0	41.0	27.0	41.0
39	35.76693585622149	39.0	35.0	41.0	27.0	41.0
40	35.703188518759916	39.0	35.0	41.0	27.0	41.0
41	35.724578097447704	39.0	35.0	41.0	27.0	41.0
42	35.56192687630367	39.0	35.0	41.0	26.0	41.0
43	35.55526225103646	39.0	35.0	40.0	26.0	41.0
44	35.333223584115096	38.0	35.0	40.0	25.0	41.0
45	35.25033764682958	38.0	35.0	40.0	24.0	41.0
46	35.260353302891524	38.0	35.0	40.0	24.0	41.0
47	35.13776854561808	38.0	35.0	40.0	24.0	41.0
48	35.14789747055249	38.0	35.0	40.0	24.0	41.0
49	35.11051778273303	38.0	34.0	40.0	24.0	41.0
50	34.48735276246462	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	1.0
13	5.0
14	7.0
15	8.0
16	39.0
17	105.0
18	252.0
19	642.0
20	1247.0
21	2201.0
22	3554.0
23	5437.0
24	8439.0
25	12334.0
26	16333.0
27	18570.0
28	18533.0
29	19140.0
30	22404.0
31	28002.0
32	36288.0
33	48864.0
34	74434.0
35	118950.0
36	102300.0
37	159866.0
38	185281.0
39	158531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.00239208500157	22.870713181137084	28.634466949966836	16.492427783894513
2	33.384176338516504	24.382468666278225	29.826669827956103	12.406685167249169
3	26.88206310612046	24.880947695698374	35.680654732479084	12.55633446570209
4	23.85144883366658	26.548015922915734	35.869180211736	13.731355031681689
5	22.476383920043695	31.086162095435743	33.530945919872835	12.90650806464773
6	19.49213309284496	39.598125880113535	30.525097214449655	10.38464381259185
7	84.98477109608751	3.267231027223885	10.235090504708817	1.5129073719797765
8	43.108980973709144	51.34756361535043	4.673204904350197	0.8702505065902326
9	82.22177853247697	4.4058711672165325	10.742976609977836	2.629373690328662
10	43.894471807089666	31.51293616915074	15.633312183411102	8.959279840348485
11	35.123909427137875	21.984336258805932	29.88052053766238	13.011233776393807
12	31.996440669668612	22.359659387061814	29.7885615717112	15.855338371558378
13	20.58325790074383	33.83600395097186	31.06274039638346	14.517997751900854
14	15.848715022236215	36.218201923843004	32.61250814719962	15.320574906721163
15	14.290980054119483	24.671400281636334	46.091983923499356	14.945635740744828
16	16.745842888394645	22.387592642898763	43.45589089327864	17.41067357542795
17	17.451757539339336	21.926069982884883	32.49808738789501	28.12408508988077
18	20.152164251383944	24.0053217171945	36.40922315791696	19.433290873504586
19	24.72477103849318	25.49576729582086	30.43582598445529	19.343635681230676
20	26.558382904463468	25.04835524958028	29.752085155154358	18.641176690801895
21	21.438437887861898	27.394748739883795	32.47917724562739	18.68763612662692
22	20.805380079460996	23.956750488831975	31.667864948947415	23.570004482759614
23	17.92009552981515	28.68390209345834	31.62361329622978	21.772389080496733
24	18.078479970127734	25.056706429160396	38.970539534196156	17.894274066515706
25	17.041109881365255	27.167155098683104	36.44022811199028	19.35150690796136
26	17.08776129833005	32.39777724236371	31.434223901843882	19.080237557462354
27	17.7056525966889	30.535176224287724	33.38388836680684	18.37528281221653
28	15.79505629366971	27.779190972278883	39.380803229890695	17.044949504160712
29	16.95990185924135	24.41846512998563	38.837304623193816	19.784328387579205
30	18.935483778073642	27.907818335926677	34.04123178938901	19.11546609661067
31	24.56302692823457	26.195922512572366	29.343933251997324	19.897117307195742
32	25.80168924204886	27.27629637664396	29.639200244967935	17.282814136339248
33	23.80940496405633	27.244235526301896	29.664733736557718	19.281625773084052
34	19.126025059298176	27.588841672194125	30.833898877774246	22.451234390733454
35	19.635255032545604	26.65216569124249	32.09377510753343	21.61880416867847
36	25.007943219658102	26.196594446561573	29.513548588986616	19.281913744793712
37	19.33490053937101	30.556390140232626	30.6547804743662	19.453928846030166
38	19.76109866966669	30.861256190191877	28.051420228476754	21.326224911664678
39	19.603290172773427	29.961632569216402	29.80248020434473	20.632597053665446
40	22.520731563331218	26.081597743837648	28.600966241076474	22.79670445175466
41	18.422222200890985	25.635625556145364	29.47419245533319	26.467959787630463
42	21.661232000568265	26.90068527667842	27.60496808793504	23.833114634818276
43	21.06705037297136	27.40713152339914	28.844590307448197	22.681227796181304
44	19.894045608959377	29.98275049459141	30.20871229610403	19.914491600345183
45	18.429133521922804	32.2579189820392	27.476436714857133	21.836510781180856
46	21.60862916827051	30.089396017735222	28.53482873842474	19.767146075569535
47	20.355088316123823	27.048606744873382	29.715896710307177	22.88040822869561
48	21.817984601192776	24.82623307086312	31.629180749283194	21.72660157866091
49	20.316404116459598	24.252593425221907	32.812840466552565	22.618161991765927
50	18.61938683143768	29.869385631555556	29.971135635635154	21.540091901371607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	348.0
1	646.5
2	945.0
3	9696.0
4	18447.0
5	12821.5
6	7196.0
7	7326.0
8	7456.0
9	7684.0
10	7912.0
11	7918.0
12	7924.0
13	7636.0
14	7348.0
15	6896.0
16	6444.0
17	6236.0
18	6028.0
19	5838.5
20	5649.0
21	6064.5
22	6480.0
23	7635.0
24	8790.0
25	10134.0
26	11478.0
27	13290.5
28	15103.0
29	17621.5
30	20140.0
31	22732.0
32	25324.0
33	28465.5
34	31607.0
35	35473.5
36	39340.0
37	44497.0
38	49654.0
39	59478.5
40	69303.0
41	79937.5
42	90572.0
43	95019.5
44	99467.0
45	100228.0
46	100989.0
47	97202.5
48	93416.0
49	87516.5
50	81617.0
51	71491.5
52	61366.0
53	53746.0
54	46126.0
55	40364.5
56	34603.0
57	30547.5
58	26492.0
59	23225.5
60	19959.0
61	17298.0
62	14637.0
63	11627.0
64	8617.0
65	6601.5
66	4586.0
67	3535.0
68	2484.0
69	2118.5
70	1753.0
71	1449.0
72	1145.0
73	833.5
74	522.0
75	375.0
76	228.0
77	183.0
78	138.0
79	102.5
80	67.0
81	50.5
82	34.0
83	23.5
84	13.0
85	13.5
86	14.0
87	10.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1041769.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.47107269196877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.73924899184146	26.156102612407935
2	6.892008629974384	4.889338782149949
3	3.3140719077921315	3.526610566431189
4	2.2018567089647645	3.1240887752395317
5	1.7490733783818897	3.1020754474085703
6	1.4050072781112892	2.990226917677843
7	1.2054041614179378	2.9929885043000124
8	1.0763065109702596	3.0542197191572287
9	0.9282197300317586	2.9632454566268516
>10	7.42353590120369	43.8243479313164
>50	0.053573627245525106	1.2294071548551508
>100	0.010333502665715406	0.7734967518958321
>500	8.15802839512253E-4	0.2232038260587352
>1k	0.0	0.0
>5k	5.438685596748353E-4	1.150647554474785
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6130	0.58842219340372	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5799	0.5566493147713168	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04655542639491096	0.0
2	0.0	0.0	0.0	0.1297792504864322	0.0
3	0.0	0.0	0.0	0.20570779126658598	0.0
4	0.0	0.0	0.0	0.29929859690584	0.0
5	0.0	0.0	0.0	0.5266042663968692	0.0
6	0.0	0.0	0.0	0.8150559289055443	0.0
7	0.0	0.0	0.0	1.0136604180005355	0.0
8	0.0	0.0	0.0	1.4313153875763245	0.0
9	0.0	0.0	0.0	1.8232448844225544	0.0
10	0.0	0.0	0.0	2.421554106524575	0.0
11	0.0	0.0	0.0	2.8652225205395823	0.0
12	0.0	0.0	0.0	3.325401312575053	0.0
13	0.0	0.0	0.0	3.527461462185955	0.0
14	0.0	0.0	0.0	3.628923494555895	0.0
15	0.0	0.0	0.0	3.7525593485695965	0.0
16	0.0	0.0	0.0	3.9577871869867502	0.0
17	0.0	0.0	0.0	4.21667375397041	0.0
18	0.0	0.0	0.0	4.563487682970025	0.0
19	0.0	0.0	0.0	4.756140756732059	0.0
20	0.0	0.0	0.0	4.944090292569658	0.0
21	0.0	0.0	0.0	5.163236763620342	0.0
22	0.0	0.0	0.0	5.396877810723875	0.0
23	0.0	0.0	0.0	5.666227349825153	0.0
24	0.0	0.0	0.0	5.87270306565083	0.0
25	0.0	0.0	0.0	6.044910148027058	0.0
26	0.0	0.0	0.0	6.212125720769191	0.0
27	0.0	0.0	0.0	6.396043652671562	0.0
28	0.0	0.0	0.0	6.576889886337566	0.0
29	0.0	0.0	0.0	6.774246498024034	0.0
30	0.0	0.0	0.0	7.036204763244059	0.0
31	0.0	0.0	0.0	7.253335432327128	0.0
32	0.0	0.0	0.0	7.453859732819848	0.0
33	9.599056988641435E-5	0.0	0.0	7.645264929173358	0.0
34	9.599056988641435E-5	0.0	0.0	7.846653144795055	0.0
35	9.599056988641435E-5	0.0	0.0	8.095076739661096	0.0
36	9.599056988641435E-5	0.0	0.0	8.30836778594871	0.0
37	9.599056988641435E-5	0.0	0.0	8.531257889224962	0.0
38	9.599056988641435E-5	0.0	0.0	8.758179596436445	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	20	7.856141E-4	44.000004	6
TCGCAAG	20	7.856141E-4	44.000004	1
CGCATGA	30	2.5274876E-6	44.000004	2
CGTCGAG	20	7.856141E-4	44.000004	3
TCGACTC	20	7.856141E-4	44.000004	20
ATCGATC	20	7.856141E-4	44.000004	43
TTCGGAT	25	4.4425527E-5	44.0	15
TATCGGT	25	4.4425527E-5	44.0	40
TCTATCG	25	4.4425527E-5	44.0	38
TCGCGGA	45	4.802132E-10	43.999996	2
TAGTACG	45	4.802132E-10	43.999996	1
CGTTTTT	7360	0.0	43.222824	1
TATGCGA	100	0.0	41.8	2
ACGGGAC	190	0.0	41.68421	5
CACGACG	165	0.0	41.333332	26
CGACGGT	160	0.0	41.250004	28
TGTTACG	70	0.0	40.857143	1
GCGTAAG	70	0.0	40.857143	1
CGTAAGA	65	0.0	40.615383	2
AACGTTG	55	7.8216544E-11	40.0	1
>>END_MODULE