FastQCFastQC Report
Thu 26 May 2016
SRR1546445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546445_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1597128
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121860.7629945752625963No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111780.6998812869100034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTACG302.5283389E-644.01
CGTTTTT139100.043.572971
GTTTTTA79750.039.917242
CGGTCTA2550.038.82353231
GCGCGAC10200.038.392169
TCACGAC2650.038.1886825
GATCGAC357.2919884E-637.7142879
TGTACGA1750.037.7142872
TAAGCGA1750.037.7142872
GAGCGAT9100.037.7142837
GACCGAT4950.037.3333329
AGCGCGA5250.037.295248
CGACGGT2800.036.92857428
TAGGGCG3100.036.9032255
TTATTTT88150.036.812256
TATTTTT88750.036.6625377
ACGGGAC2650.036.52835
TCGTCCC5250.036.45714638
CACGACG2800.036.14285726
CGCGACC10850.036.09216710
Produced by FastQC (version 0.11.5)