##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546445_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1597128
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.552111665439465	31.0	31.0	34.0	30.0	34.0
2	31.930569747697117	31.0	31.0	34.0	30.0	34.0
3	32.001456990297584	33.0	31.0	34.0	30.0	34.0
4	35.70441755451034	37.0	35.0	37.0	35.0	37.0
5	35.664777024759445	37.0	35.0	37.0	33.0	37.0
6	35.708079127033024	37.0	35.0	37.0	33.0	37.0
7	36.05167087421923	37.0	35.0	37.0	35.0	37.0
8	18.75658932784348	32.0	0.0	37.0	0.0	37.0
9	28.10254844946679	32.0	17.0	39.0	17.0	39.0
10	33.747301406023816	33.0	32.0	39.0	27.0	39.0
11	35.879377858255566	37.0	35.0	39.0	32.0	39.0
12	36.57536152393546	37.0	35.0	39.0	33.0	39.0
13	36.76188821434475	39.0	35.0	39.0	33.0	39.0
14	37.92901883881567	40.0	37.0	41.0	33.0	41.0
15	38.1484552271327	40.0	37.0	41.0	33.0	41.0
16	38.20321163989361	40.0	37.0	41.0	33.0	41.0
17	38.1463583382171	40.0	37.0	41.0	33.0	41.0
18	38.07589185087232	40.0	37.0	41.0	33.0	41.0
19	38.043030990628175	40.0	37.0	41.0	34.0	41.0
20	37.9091801032854	40.0	36.0	41.0	33.0	41.0
21	37.78477304261149	40.0	36.0	41.0	33.0	41.0
22	37.793173746875645	40.0	36.0	41.0	33.0	41.0
23	37.642189605341585	39.0	35.0	41.0	33.0	41.0
24	37.45269070481514	39.0	35.0	41.0	33.0	41.0
25	37.31254038499106	39.0	35.0	41.0	33.0	41.0
26	37.27459727711242	39.0	35.0	41.0	33.0	41.0
27	37.14443300724801	39.0	35.0	41.0	32.0	41.0
28	37.101211674956545	39.0	35.0	41.0	32.0	41.0
29	36.96870382336294	39.0	35.0	41.0	32.0	41.0
30	36.807427457285826	39.0	35.0	40.0	31.0	41.0
31	36.73893075570649	39.0	35.0	41.0	31.0	41.0
32	36.43846767447568	39.0	35.0	41.0	30.0	41.0
33	36.21146395279527	39.0	35.0	41.0	30.0	41.0
34	35.95422971734263	39.0	35.0	41.0	28.0	41.0
35	35.715145561282505	39.0	35.0	41.0	26.0	41.0
36	35.56765706943965	39.0	35.0	41.0	25.0	41.0
37	35.499572983505395	39.0	35.0	41.0	25.0	41.0
38	35.38973645193121	39.0	35.0	41.0	25.0	41.0
39	35.32399845222174	39.0	35.0	40.0	24.0	41.0
40	35.22320064515806	38.0	35.0	40.0	24.0	41.0
41	35.17970882734508	38.0	35.0	40.0	24.0	41.0
42	35.02800965232593	38.0	35.0	40.0	23.0	41.0
43	35.04843068307612	38.0	35.0	40.0	23.0	41.0
44	34.80553405863525	38.0	34.0	40.0	23.0	41.0
45	34.72129848077299	38.0	34.0	40.0	23.0	41.0
46	34.71405109671861	38.0	34.0	40.0	23.0	41.0
47	34.60352081987167	38.0	34.0	40.0	23.0	41.0
48	34.577321917842525	38.0	34.0	40.0	23.0	41.0
49	34.540344293005944	38.0	34.0	40.0	23.0	41.0
50	33.94823332882524	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	2.0
12	6.0
13	8.0
14	16.0
15	33.0
16	70.0
17	174.0
18	479.0
19	1133.0
20	2066.0
21	3705.0
22	5788.0
23	8882.0
24	13686.0
25	21005.0
26	28703.0
27	32862.0
28	32821.0
29	33082.0
30	37622.0
31	46034.0
32	58358.0
33	76568.0
34	114797.0
35	168694.0
36	163074.0
37	246282.0
38	265755.0
39	235419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.22027288983726	22.83285998367069	25.485371241378274	19.46149588511378
2	36.03474486703633	23.677000215386617	27.863327172274232	12.424927745302819
3	28.038266187807114	23.8433613335938	35.50416748062772	12.61420499797136
4	24.624200439789423	25.996476174733647	34.98191754198787	14.397405843489064
5	22.004498074042907	30.082059797336218	33.813382521626316	14.100059606994556
6	19.114498023952994	38.815674135072456	31.333681458217498	10.736146382757049
7	82.4566346592133	3.534719822080635	12.04875251075681	1.9598930079492691
8	43.32138688946659	49.39253459960629	5.954563441377273	1.331515069549842
9	78.66983735805772	4.632815904548665	13.361671700702763	3.33567503669086
10	39.504723478644166	28.9092045221172	19.070293677150485	12.515778322088147
11	32.82172750086405	22.14468721354832	29.748398375083273	15.285186910504356
12	30.554157212195893	20.52628217650683	31.498289429526	17.42127118177128
13	23.27552957558818	26.154572457561322	32.34336884708051	18.22652911976999
14	18.083397197970356	29.538897320690637	33.78314073762404	18.59456474371497
15	17.4145090437335	23.34415275419378	41.08048947861411	18.16084872345861
16	20.58945807724866	21.432972185072206	39.32427457285828	18.653295164820854
17	20.254356570043228	21.859049493841447	33.12439579044384	24.76219814567148
18	21.937628042335994	23.693342048977918	34.37031972390441	19.998710184781686
19	25.11082392895247	25.969865909307206	29.798676123641936	19.120634038098387
20	26.435702085243012	24.925428644416726	29.673827019500003	18.96504225084026
21	23.291620959622524	26.28593325018408	31.87859708176176	18.543848708431636
22	21.92642042466227	23.2964421135939	32.46051662734609	22.316620834397742
23	20.637231330237775	26.22695237952124	31.873838540179623	21.26197775006136
24	21.61248190501951	24.093748278159296	34.864143637829905	19.42962617899129
25	21.492203505292	24.965437961140246	32.66106411007759	20.881294423490164
26	18.870998442203756	28.167685996363474	31.368243497077252	21.593072064355518
27	19.48485030629981	25.700069124077718	33.08194459054002	21.73313597908245
28	19.123889882338798	25.746903191228256	35.44869290376225	19.680514022670696
29	20.23807734884117	25.013148601740127	34.584141033154516	20.164633016264194
30	21.55681949098632	25.70814612228951	33.331392349266935	19.403642037457235
31	25.28582555687459	24.955482591251297	29.81996433598309	19.938727515891024
32	25.58348485531529	25.963166383658663	29.546097745453086	18.907251015572953
33	25.05804168482426	26.485979833801675	28.87927580006111	19.57670268131296
34	21.417193862984057	26.69360251651715	31.431544622597563	20.45765899790123
35	21.72743825166173	26.956762388487338	30.690213934011553	20.625585425839382
36	25.775391828331855	27.066271457265795	27.788630591912483	19.36970612248987
37	23.93809387851193	28.786045952484713	27.774229742387586	19.501630426615776
38	23.56348395369689	29.006942461718786	26.82596510736773	20.603608477216603
39	23.819443400904625	27.38027258929779	27.119742437675626	21.68054157212196
40	24.119857644471825	24.64511297779514	28.962487665359323	22.272541712373712
41	21.374304376355557	24.491023887878743	29.315058029162344	24.819613706603352
42	22.229652225745212	27.212721835695074	26.683083635125048	23.874542303434666
43	21.413875406354407	26.372025285387267	28.06556518951518	24.148534118743147
44	21.97863915728733	26.69642007403289	29.352625462705557	21.972315305974224
45	21.00413993117646	28.137506824750425	27.929758917256475	22.928594326816636
46	22.490683276481285	27.804408914000632	28.746787984432054	20.95811982508603
47	21.71516622337095	26.396694566747314	29.336909753006644	22.55122945687509
48	22.167665960398917	24.91584894886321	30.479398019444904	22.43708707129297
49	22.440593365090336	24.040966034031086	30.530301891895956	22.988138708982625
50	20.245590835549812	25.990903672091402	30.688711236669825	23.074794255688964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	645.0
1	1377.5
2	2110.0
3	20195.0
4	38280.0
5	26296.0
6	14312.0
7	14137.0
8	13962.0
9	13956.0
10	13950.0
11	13755.5
12	13561.0
13	12978.0
14	12395.0
15	11604.5
16	10814.0
17	10210.5
18	9607.0
19	9339.5
20	9072.0
21	9023.5
22	8975.0
23	9507.5
24	10040.0
25	11720.0
26	13400.0
27	15881.5
28	18363.0
29	21238.5
30	24114.0
31	27824.0
32	31534.0
33	35083.0
34	38632.0
35	43865.0
36	49098.0
37	54488.0
38	59878.0
39	70104.0
40	80330.0
41	90264.0
42	100198.0
43	105829.0
44	111460.0
45	116095.0
46	120730.0
47	125058.5
48	129387.0
49	126078.5
50	122770.0
51	111528.0
52	100286.0
53	91373.0
54	82460.0
55	77459.0
56	72458.0
57	70476.5
58	68495.0
59	65095.5
60	61696.0
61	56493.5
62	51291.0
63	43668.5
64	36046.0
65	29497.5
66	22949.0
67	19224.0
68	15499.0
69	13623.5
70	11748.0
71	9871.5
72	7995.0
73	6168.0
74	4341.0
75	3215.0
76	2089.0
77	1648.0
78	1207.0
79	880.0
80	553.0
81	389.5
82	226.0
83	166.5
84	107.0
85	68.5
86	30.0
87	20.5
88	11.0
89	9.0
90	7.0
91	7.5
92	8.0
93	7.0
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1597128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.168515141097686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.73717172164322	25.22889809872135
2	7.076900714471518	4.9776817985787325
3	3.697882527299788	3.9014711295402953
4	2.6119532679080915	3.674340722010612
5	2.078097741248718	3.6541805938893233
6	1.6905623977265156	3.5672741568849218
7	1.4264049912821282	3.5115181873269985
8	1.2915773829623847	3.6338286998889564
9	1.0810636471215425	3.421746291805583
>10	7.247043788922151	39.912532912292484
>50	0.03932856797541994	0.9360827213328294
>100	0.01942346626026683	1.4454902329229555
>500	0.001664861900262146	0.38910206887248755
>1k	5.549539667540486E-4	0.22587567014299897
>5k	0.0	0.0
>10k+	3.6996931116936577E-4	1.5199767157894666
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12186	0.7629945752625963	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11178	0.6998812869100034	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.032871504350308806	0.0
2	0.0	0.0	0.0	0.10838204577216103	0.0
3	0.0	0.0	0.0	0.1999839712283549	0.0
4	0.0	0.0	0.0	0.28757870377327305	0.0
5	0.0	0.0	0.0	0.4963910218842823	0.0
6	0.0	0.0	0.0	0.8947310422207864	0.0
7	0.0	0.0	0.0	1.1508157142070015	0.0
8	0.0	0.0	0.0	1.6584143537650082	0.0
9	0.0	0.0	0.0	2.218169113558838	0.0
10	0.0	0.0	0.0	2.9017711792667837	0.0
11	0.0	0.0	0.0	3.3260953411373415	0.0
12	0.0	0.0	0.0	3.6698373580577135	0.0
13	0.0	0.0	0.0	3.847593931106336	0.0
14	0.0	0.0	0.0	3.9477737538882294	0.0
15	0.0	0.0	0.0	4.033803176702181	0.0
16	0.0	0.0	0.0	4.17317835514749	0.0
17	0.0	0.0	0.0	4.32964671585496	0.0
18	0.0	0.0	0.0	4.581160683426751	0.0
19	0.0	0.0	0.0	4.698934587584715	0.0
20	0.0	0.0	0.0	4.847451174858872	0.0
21	0.0	0.0	0.0	4.987765539142761	0.0
22	0.0	0.0	0.0	5.145423535245766	0.0
23	0.0	0.0	0.0	5.325308929528504	0.0
24	0.0	0.0	0.0	5.461052589397969	0.0
25	0.0	0.0	0.0	5.578701268777455	0.0
26	0.0	0.0	0.0	5.697915257887908	0.0
27	0.0	0.0	0.0	5.826333268216448	0.0
28	0.0	0.0	0.0	5.956128751108239	0.0
29	0.0	0.0	0.0	6.099824184411018	0.0
30	0.0	0.0	0.0	6.283403709658837	0.0
31	0.0	0.0	0.0	6.427099142961616	0.0
32	0.0	0.0	0.0	6.577306264745218	0.0
33	6.261238923868344E-5	0.0	0.0	6.726511588301	0.0
34	6.261238923868344E-5	0.0	0.0	6.880538065828161	0.0
35	6.261238923868344E-5	0.0	0.0	7.05422483357627	0.0
36	6.261238923868344E-5	0.0	0.0	7.2155769606443565	0.0
37	6.261238923868344E-5	0.0	0.0	7.389827239895612	0.0
38	6.261238923868344E-5	0.0	0.0	7.611537710189791	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTACG	30	2.5283389E-6	44.0	1
CGTTTTT	13910	0.0	43.57297	1
GTTTTTA	7975	0.0	39.91724	2
CGGTCTA	255	0.0	38.823532	31
GCGCGAC	1020	0.0	38.39216	9
TCACGAC	265	0.0	38.18868	25
GATCGAC	35	7.2919884E-6	37.714287	9
TGTACGA	175	0.0	37.714287	2
TAAGCGA	175	0.0	37.714287	2
GAGCGAT	910	0.0	37.714283	7
GACCGAT	495	0.0	37.333332	9
AGCGCGA	525	0.0	37.29524	8
CGACGGT	280	0.0	36.928574	28
TAGGGCG	310	0.0	36.903225	5
TTATTTT	8815	0.0	36.81225	6
TATTTTT	8875	0.0	36.662537	7
ACGGGAC	265	0.0	36.5283	5
TCGTCCC	525	0.0	36.457146	38
CACGACG	280	0.0	36.142857	26
CGCGACC	1085	0.0	36.092167	10
>>END_MODULE