FastQCFastQC Report
Thu 26 May 2016
SRR1546444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546444_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1018342
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70810.6953459643223986No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64260.6310257261312997No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC13160.1292296694037956No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTG207.856059E-444.01
TAAGCGA408.310963E-944.02
GTAACGA408.310963E-944.02
TAGTACG302.5274312E-644.01
CGTTTTT76150.042.9310571
CGGTCTA1400.040.8571431
GTTTTTA40850.040.3378222
GCGATTC603.6379788E-1240.3333329
CGACGGT1600.039.87528
TACGGGA1300.038.923084
GAGCGAT5600.038.8928537
TATGCGA800.038.52
CGAGGTA404.1239218E-738.56
TGTACGA404.1239218E-738.52
TAATGCG404.1239218E-738.51
GCGCGAC8200.038.3658529
CTATCTC4800.038.0416646
TTGTGCG357.289391E-637.7142831
TGTTACG357.289391E-637.7142831
ACGCTCC357.289391E-637.71428314
Produced by FastQC (version 0.11.5)