##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546444_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1018342
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.507321705281722	31.0	31.0	34.0	30.0	34.0
2	31.882098548424793	31.0	31.0	34.0	30.0	34.0
3	31.96424874943781	31.0	31.0	34.0	30.0	34.0
4	35.67381979727832	37.0	35.0	37.0	33.0	37.0
5	35.638561504877536	37.0	35.0	37.0	33.0	37.0
6	35.67926492278625	37.0	35.0	37.0	33.0	37.0
7	36.00522614210157	37.0	35.0	37.0	35.0	37.0
8	18.30121609439658	28.0	0.0	37.0	0.0	37.0
9	27.822476142592567	26.0	17.0	39.0	17.0	39.0
10	33.63205975988421	32.0	32.0	39.0	27.0	39.0
11	35.81772724683849	37.0	35.0	39.0	32.0	39.0
12	36.47167552747505	37.0	35.0	39.0	32.0	39.0
13	36.64476276142985	39.0	35.0	39.0	33.0	39.0
14	37.731273972791065	39.0	36.0	41.0	33.0	41.0
15	37.97448597818808	40.0	36.0	41.0	33.0	41.0
16	38.04441729792153	40.0	36.0	41.0	33.0	41.0
17	37.98290161851323	40.0	36.0	41.0	33.0	41.0
18	37.92024192265467	40.0	36.0	41.0	33.0	41.0
19	37.90109216746437	40.0	36.0	41.0	33.0	41.0
20	37.76611393814652	40.0	36.0	41.0	33.0	41.0
21	37.646302519192965	39.0	35.0	41.0	33.0	41.0
22	37.65038268086753	39.0	35.0	41.0	33.0	41.0
23	37.52336346728309	39.0	35.0	41.0	33.0	41.0
24	37.34569132963189	39.0	35.0	41.0	33.0	41.0
25	37.20742049331168	39.0	35.0	41.0	32.0	41.0
26	37.1598863643059	39.0	35.0	41.0	32.0	41.0
27	37.04628012985814	39.0	35.0	41.0	32.0	41.0
28	37.0024687187605	39.0	35.0	41.0	32.0	41.0
29	36.85381040946951	39.0	35.0	40.0	32.0	41.0
30	36.70814520072824	38.0	35.0	40.0	31.0	41.0
31	36.65658491940822	39.0	35.0	41.0	31.0	41.0
32	36.413603681278	39.0	35.0	41.0	30.0	41.0
33	36.2259771275269	39.0	35.0	41.0	30.0	41.0
34	35.98603023345792	39.0	35.0	41.0	29.0	41.0
35	35.776524978838154	39.0	35.0	41.0	27.0	41.0
36	35.63057401148141	39.0	35.0	41.0	27.0	41.0
37	35.58151387254969	38.0	35.0	40.0	26.0	41.0
38	35.459436024439725	38.0	35.0	40.0	26.0	41.0
39	35.3935446048577	38.0	35.0	40.0	26.0	41.0
40	35.302156839254394	38.0	35.0	40.0	25.0	41.0
41	35.26165374697302	38.0	35.0	40.0	25.0	41.0
42	35.098384432734775	38.0	34.0	40.0	24.0	41.0
43	35.08375378802013	38.0	34.0	40.0	24.0	41.0
44	34.83074939460417	38.0	34.0	40.0	23.0	41.0
45	34.71979845670708	38.0	34.0	40.0	23.0	41.0
46	34.717826624061466	38.0	34.0	40.0	23.0	41.0
47	34.5970518745176	37.0	34.0	40.0	23.0	41.0
48	34.56337949333328	37.0	34.0	40.0	23.0	41.0
49	34.528251805385615	37.0	34.0	40.0	24.0	41.0
50	33.944265286121954	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	1.0
12	5.0
13	6.0
14	9.0
15	28.0
16	53.0
17	127.0
18	337.0
19	675.0
20	1343.0
21	2305.0
22	3622.0
23	5589.0
24	8123.0
25	12424.0
26	16706.0
27	19868.0
28	20596.0
29	21575.0
30	25092.0
31	30929.0
32	39587.0
33	52247.0
34	78178.0
35	113913.0
36	107819.0
37	157975.0
38	165555.0
39	133652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.45722753259711	22.081776063444305	26.898723611517543	17.562272792441046
2	36.62973735739074	23.112274658218947	27.458849777383236	12.799138207007077
3	28.85366605717922	24.313541030420037	34.05083950185694	12.781953410543807
4	25.516869578196715	26.01935302678275	33.4777510895161	14.986026305504438
5	22.197945287536015	30.05620901426044	32.60093367454156	15.144912023661991
6	20.046801565682255	39.23701467679817	29.85107164390745	10.865112113612128
7	82.61615449426617	3.9170534064194547	11.229527997470399	2.2372641018439774
8	42.15293094068594	50.68444589342283	5.59880668773359	1.5638164781576327
9	78.5504280487302	4.602677685885488	13.016059437792018	3.830834827592302
10	43.3151141757877	26.202493857662752	17.5654151552229	12.916976811326647
11	36.7398182535926	21.494743416258977	26.35990659326631	15.405531736882109
12	32.9908812560024	19.54530010546555	30.045799937545542	17.418018700986508
13	25.292387036967934	24.93317569146711	31.22379318539351	18.550644086171445
14	18.92939700022193	28.642440358936387	33.766259272425174	18.661903368416503
15	19.089657502096546	22.092381537833067	40.69320522967726	18.12475573039313
16	22.96743137374281	19.749357288612273	38.22114770872654	19.06206362891838
17	21.80043639563133	20.746468278829706	32.04041471332813	25.41268061221083
18	23.98702989761789	22.454735246115746	33.247474816908266	20.310760039358094
19	27.02049016931444	25.820500381993476	28.07210151402967	19.086907934662424
20	28.093410661644125	25.084794695691627	27.049262428535798	19.772532214128454
21	25.23209295109109	26.346158756095694	29.959188563370653	18.462559729442564
22	22.78262116263495	22.562066574883488	30.943631903623736	23.71168035885783
23	22.004002584593387	25.96298689438322	29.905179203057518	22.12783131796587
24	23.274302739158355	23.89482118973783	33.05952224301855	19.77135382808526
25	23.622123019574957	22.82887281483038	31.289684604975537	22.259319560619122
26	19.711845332903877	26.974729511303668	30.10452284203146	23.208902313760994
27	20.942374958510992	24.050171749765795	32.03030023312404	22.97715305859917
28	20.29180766383003	25.096578556123582	34.19234402587736	20.41926975416903
29	22.245866319959305	25.251045326619153	32.207450934951126	20.295637418470417
30	24.35625752448588	23.897767154845816	31.369323861728184	20.37665145894012
31	27.941006066724146	22.231627488604026	28.02270749905238	21.804658945619448
32	28.294325482008993	24.745124918740462	27.25047184541146	19.710077753839084
33	27.522188027205008	23.655707021806034	26.943207684648186	21.878897266340775
34	22.1020050238525	26.449267534875315	29.649371232847116	21.799356208425067
35	22.093756321550128	26.18511266352561	29.45896368803408	22.262167326890182
36	25.888061181803362	27.463857918066818	25.881481859728854	20.766599040400965
37	23.739961623894526	28.69360195297847	25.44567542142031	22.120761001706697
38	23.229916864864652	29.64180992240328	24.681197475897097	22.44707573683497
39	23.000524381789223	26.10999055327189	25.797914649498892	25.09157041544
40	23.609848164958333	22.685109717560508	28.57910210911462	25.12594000836654
41	20.329908812560024	23.28166765192833	28.50329260700236	27.885130928509284
42	22.036310001944337	25.902398211995575	25.965049069958816	26.09624271610127
43	21.287445671493465	24.754650205922964	27.47200842153226	26.485895701051316
44	22.58966044806165	25.439096099345797	28.598152683479615	23.37309076911293
45	21.799552606098935	26.830377221012192	27.31606866848269	24.05400150440618
46	23.385463822566486	26.144752941546162	28.104310732543684	22.36547250334367
47	22.113887083121387	25.180833158212074	28.488464582625483	24.216815176041052
48	22.1740829701613	23.91072940132097	29.95801017732746	23.95717745119027
49	23.27764149961408	22.44236219266219	29.876996136857752	24.403000170865976
50	20.911638722550972	24.87847893929544	30.004851022544486	24.205031315609098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	333.0
1	864.5
2	1396.0
3	11760.0
4	22124.0
5	14956.0
6	7788.0
7	7575.5
8	7363.0
9	7514.0
10	7665.0
11	7614.5
12	7564.0
13	7206.5
14	6849.0
15	6383.5
16	5918.0
17	5654.0
18	5390.0
19	5127.5
20	4865.0
21	4867.5
22	4870.0
23	5006.5
24	5143.0
25	5815.5
26	6488.0
27	7451.5
28	8415.0
29	9741.0
30	11067.0
31	12673.0
32	14279.0
33	16182.0
34	18085.0
35	20533.5
36	22982.0
37	25929.0
38	28876.0
39	34783.5
40	40691.0
41	48509.0
42	56327.0
43	61479.0
44	66631.0
45	71921.5
46	77212.0
47	80780.0
48	84348.0
49	82153.0
50	79958.0
51	72738.5
52	65519.0
53	62260.0
54	59001.0
55	57478.5
56	55956.0
57	54961.5
58	53967.0
59	52248.0
60	50529.0
61	46715.0
62	42901.0
63	36495.5
64	30090.0
65	24960.0
66	19830.0
67	16767.0
68	13704.0
69	11847.0
70	9990.0
71	8382.5
72	6775.0
73	5304.5
74	3834.0
75	2887.0
76	1940.0
77	1414.0
78	888.0
79	642.5
80	397.0
81	307.5
82	218.0
83	149.5
84	81.0
85	64.5
86	48.0
87	36.5
88	25.0
89	19.5
90	14.0
91	9.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1018342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.10281174118766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.15981084882313	26.65240251936491
2	8.521387558265877	6.664204269291388
3	4.933541601846679	5.787460454229843
4	3.632752064627066	5.682032803420918
5	2.8780455169773247	5.626983601646672
6	2.283585637480811	5.3576771566375285
7	1.917945725256645	5.249794943716836
8	1.5956277689526799	4.991482580669433
9	1.2883399589802067	4.5339943387187205
>10	4.735072523026287	25.45796316548846
>50	0.032487457924115115	0.8560371823492962
>100	0.02014431798872467	1.5356201112373227
>500	5.036079401833229E-4	0.1448382380335967
>1k	2.5180397009166145E-4	0.12957656101442097
>5k	5.036079401833229E-4	1.3299320741806868
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7081	0.6953459643223986	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6426	0.6310257261312997	No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1316	0.1292296694037956	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02857586154749583	0.0
2	0.0	0.0	0.0	0.09014653230447138	0.0
3	0.0	0.0	0.0	0.1642866541888678	0.0
4	0.0	0.0	0.0	0.24854125627736065	0.0
5	0.0	0.0	0.0	0.4632039138128448	0.0
6	0.0	0.0	0.0	0.8654263498903119	0.0
7	0.0	0.0	0.0	1.136062344477592	0.0
8	0.0	0.0	0.0	1.7284959276942324	0.0
9	0.0	0.0	0.0	2.4614520465619605	0.0
10	0.0	0.0	0.0	3.3119521732384603	0.0
11	0.0	0.0	0.0	3.8568575193795405	0.0
12	0.0	0.0	0.0	4.242287954341469	0.0
13	0.0	0.0	0.0	4.448407313063784	0.0
14	0.0	0.0	0.0	4.558586408102583	0.0
15	0.0	0.0	0.0	4.649714928776383	0.0
16	0.0	0.0	0.0	4.79347802604626	0.0
17	0.0	0.0	0.0	4.946275416313969	0.0
18	0.0	0.0	0.0	5.19481667259133	0.0
19	0.0	0.0	0.0	5.3075489373903855	0.0
20	0.0	0.0	0.0	5.464372479972347	0.0
21	0.0	0.0	0.0	5.612358127230341	9.819883693297537E-5
22	0.0	0.0	0.0	5.777233974440807	9.819883693297537E-5
23	0.0	0.0	0.0	5.957428840212817	9.819883693297537E-5
24	0.0	0.0	0.0	6.103057715384419	9.819883693297537E-5
25	0.0	0.0	0.0	6.22786843712623	9.819883693297537E-5
26	0.0	0.0	0.0	6.3562143169976295	9.819883693297537E-5
27	0.0	0.0	0.0	6.493692688703795	9.819883693297537E-5
28	0.0	0.0	0.0	6.629010685997435	9.819883693297537E-5
29	0.0	0.0	0.0	6.773853970473573	9.819883693297537E-5
30	0.0	0.0	0.0	6.959744368787696	9.819883693297537E-5
31	0.0	0.0	0.0	7.118433689271384	9.819883693297537E-5
32	0.0	0.0	0.0	7.27309685744082	9.819883693297537E-5
33	0.0	0.0	0.0	7.442588049987136	9.819883693297537E-5
34	0.0	0.0	0.0	7.613846821598245	9.819883693297537E-5
35	0.0	0.0	0.0	7.800031816423166	9.819883693297537E-5
36	0.0	0.0	0.0	7.972174377566672	9.819883693297537E-5
37	0.0	0.0	0.0	8.166706273530897	9.819883693297537E-5
38	0.0	0.0	0.0	8.441368420432429	9.819883693297537E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTG	20	7.856059E-4	44.0	1
TAAGCGA	40	8.310963E-9	44.0	2
GTAACGA	40	8.310963E-9	44.0	2
TAGTACG	30	2.5274312E-6	44.0	1
CGTTTTT	7615	0.0	42.931057	1
CGGTCTA	140	0.0	40.85714	31
GTTTTTA	4085	0.0	40.337822	2
GCGATTC	60	3.6379788E-12	40.333332	9
CGACGGT	160	0.0	39.875	28
TACGGGA	130	0.0	38.92308	4
GAGCGAT	560	0.0	38.892853	7
TATGCGA	80	0.0	38.5	2
CGAGGTA	40	4.1239218E-7	38.5	6
TGTACGA	40	4.1239218E-7	38.5	2
TAATGCG	40	4.1239218E-7	38.5	1
GCGCGAC	820	0.0	38.365852	9
CTATCTC	480	0.0	38.041664	6
TTGTGCG	35	7.289391E-6	37.714283	1
TGTTACG	35	7.289391E-6	37.714283	1
ACGCTCC	35	7.289391E-6	37.714283	14
>>END_MODULE