FastQCFastQC Report
Thu 26 May 2016
SRR1546443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546443_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1280023
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89510.6992843097350594No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83110.649285208156416No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA351.4462603E-744.02
TACGCGG254.443124E-544.02
CGTACGA302.5279423E-644.02
TAGTCGA207.8568177E-444.02
CGTTTTT117000.043.5299151
AGCGATA1800.041.5555578
CGACGGT1400.040.85714328
TAATGCG603.6379788E-1240.3333321
GTTTTTA66050.040.003032
ATTGCGA1500.039.62
CGGTCTA1450.039.44827731
AAGTGCG452.3512257E-839.1111151
GTTAGCG1300.038.923081
GAGCGAT6700.038.746277
GGCAACG404.1250314E-738.51
TCGTCCC1150.038.26086838
CGACCCG3000.038.1333332
GACGGTC1500.038.1333329
TAGTACG750.038.133331
AGCACGA750.038.133332
Produced by FastQC (version 0.11.5)