FastQCFastQC Report
Thu 26 May 2016
SRR1546442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546442_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1402833
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35530.2532731978788637No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33560.23923018634434745No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20780.1481288221762676No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAT207.857076E-444.016
TAGCCGT254.4433444E-544.044
TTAACGA302.5281188E-644.02
TATACGA351.4463876E-744.02
CCCGATA207.857076E-444.024
CGGTCTA2350.043.06382831
CGTTTTT57100.042.574431
AGCGATA1800.040.3333328
ACGAGAT3450.040.1739165
GAGCGAT8400.040.071437
TATAGCG1000.039.61
TGCGCGA950.039.368422
CGTTAGG1850.039.2432442
AGGGGAA123300.039.1111158
CGACGGT2600.038.92307728
GAGGGGA120450.038.6849337
TCACGAC2800.038.525
TTAGCGA1500.038.133332
GTTTTTA34200.038.0175442
CGAGGTT1050.037.714296
Produced by FastQC (version 0.11.5)