##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546442_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1402833
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.622573036134735	31.0	31.0	34.0	30.0	34.0
2	31.988008551267328	31.0	31.0	34.0	30.0	34.0
3	32.087421667440104	33.0	31.0	34.0	30.0	34.0
4	35.76351924997487	37.0	35.0	37.0	35.0	37.0
5	35.704845836959926	37.0	35.0	37.0	33.0	37.0
6	35.752390341544576	37.0	35.0	37.0	35.0	37.0
7	36.096864701643035	37.0	35.0	37.0	35.0	37.0
8	18.390015775220572	30.0	0.0	37.0	0.0	37.0
9	27.947042876807146	28.0	17.0	39.0	17.0	39.0
10	33.775157128467896	32.0	32.0	39.0	27.0	39.0
11	35.90076153041738	37.0	35.0	39.0	32.0	39.0
12	36.468567534410724	37.0	35.0	39.0	33.0	39.0
13	36.5354115564718	39.0	35.0	39.0	33.0	39.0
14	37.67533484028391	39.0	36.0	41.0	33.0	41.0
15	37.98190732610368	40.0	36.0	41.0	33.0	41.0
16	38.09196176594078	40.0	36.0	41.0	34.0	41.0
17	38.068839270248134	40.0	36.0	41.0	34.0	41.0
18	38.05631532762631	40.0	36.0	41.0	34.0	41.0
19	38.04766925214904	40.0	36.0	41.0	34.0	41.0
20	37.928513230013834	40.0	36.0	41.0	34.0	41.0
21	37.80031407872498	40.0	35.0	41.0	33.0	41.0
22	37.78553327445248	40.0	35.0	41.0	33.0	41.0
23	37.67732723709807	39.0	35.0	41.0	33.0	41.0
24	37.57243021799459	39.0	35.0	41.0	33.0	41.0
25	37.461206002425094	39.0	35.0	41.0	33.0	41.0
26	37.38872766751281	39.0	35.0	41.0	33.0	41.0
27	37.24360846943293	39.0	35.0	41.0	33.0	41.0
28	37.27768593980895	39.0	35.0	41.0	33.0	41.0
29	37.21385867027651	39.0	35.0	41.0	33.0	41.0
30	37.14462591056812	39.0	35.0	41.0	32.0	41.0
31	37.124594303099514	39.0	35.0	41.0	32.0	41.0
32	36.908199336628094	39.0	35.0	41.0	32.0	41.0
33	36.82440033845796	39.0	35.0	41.0	31.0	41.0
34	36.73218408748583	39.0	35.0	41.0	31.0	41.0
35	36.57884794555018	39.0	35.0	41.0	31.0	41.0
36	36.4121581114787	39.0	35.0	41.0	30.0	41.0
37	36.326080153517914	39.0	35.0	41.0	30.0	41.0
38	36.25695645882297	39.0	35.0	41.0	30.0	41.0
39	36.21686187878386	39.0	35.0	41.0	30.0	41.0
40	36.12491080549146	39.0	35.0	40.0	30.0	41.0
41	36.14794633431064	39.0	35.0	40.0	30.0	41.0
42	36.021681839534715	39.0	35.0	40.0	30.0	41.0
43	36.02382892332872	39.0	35.0	40.0	30.0	41.0
44	35.80098700272948	38.0	35.0	40.0	29.0	41.0
45	35.71002606867674	38.0	35.0	40.0	29.0	41.0
46	35.71718444034322	38.0	35.0	40.0	29.0	41.0
47	35.61892042744931	38.0	35.0	40.0	29.0	41.0
48	35.622210911776385	38.0	35.0	40.0	29.0	41.0
49	35.59738472077574	38.0	35.0	40.0	28.0	41.0
50	34.95175334483862	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	6.0
14	8.0
15	21.0
16	45.0
17	101.0
18	254.0
19	598.0
20	1190.0
21	2217.0
22	3662.0
23	5642.0
24	8492.0
25	12405.0
26	16262.0
27	18474.0
28	20022.0
29	23020.0
30	28330.0
31	37357.0
32	50219.0
33	68188.0
34	108761.0
35	181852.0
36	143198.0
37	221469.0
38	250932.0
39	200100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.86620716792377	23.136039713921758	30.29056202698397	13.70719109117051
2	31.577814322873788	24.980093852939017	30.50748022038261	12.934611603804585
3	28.576102786290313	25.061571833568212	33.195825875211085	13.166499504930378
4	25.237002551265903	27.048408470573477	33.040140914848735	14.674448063311885
5	22.90044502802543	31.674475864197664	31.134140699570086	14.290938408206822
6	19.931452995474157	41.13148179434045	28.012671501169418	10.92439370901597
7	87.5988089815395	3.436831041185943	7.170062295369442	1.7942976819051164
8	45.23097189758153	50.38518483668405	3.200167090451964	1.1836761752824463
9	84.75884157273174	4.38676592295733	8.107593705024048	2.746798799286872
10	47.770832308621195	29.982827606707286	12.565501381846591	9.680838702824927
11	41.651215789762574	20.903272164256187	24.432772824705435	13.012739221275805
12	38.25822460692043	21.291058878711862	24.566502213734637	15.884214300633076
13	22.1737726443561	36.72618194753046	25.64410731712185	15.455938090991586
14	16.020866346885196	38.976342871888534	28.158733077992892	16.844057703233386
15	14.962579294898251	23.62298292098917	45.526231561418925	15.888206222693649
16	17.00900962552207	20.381043217546207	43.23280105329715	19.377146103634573
17	17.467011397650325	20.448050480705827	29.42267540042186	32.662262721221985
18	21.069792341640095	23.91845643779409	33.260195618437834	21.75155560212798
19	27.61682965827009	25.222032843538756	26.41775606932543	20.74338142886573
20	29.50401081240604	24.68640244419685	25.006397767945295	20.803188975451818
21	22.486069261273435	29.42916227377029	27.49258108413475	20.59218738082152
22	22.870505612571133	24.621604995035046	26.476779488363906	26.031109904029915
23	19.584155776204295	30.42585967110839	26.304485280856664	23.685499271830647
24	19.559847822228306	25.345639858771502	36.589173479665796	18.505338839334403
25	18.32577363093112	26.739034510879055	34.018090535366646	20.91710132282317
26	18.107857457017335	33.3087402420673	27.175009427351654	21.408392873563713
27	19.619869221781922	30.412030512541406	29.035957950803837	20.93214231487283
28	16.7940161088312	27.231751748069797	37.58530060242381	18.38893154067519
29	17.543071769768748	24.178715499278958	36.35892511795773	21.919287612994562
30	20.859432305912392	27.861548737447723	30.766955154319863	20.512063802320018
31	28.873500979803012	24.811221292912272	24.77087436637148	21.54440336091324
32	29.55832946615884	24.9263454737663	26.455322907288327	19.06000215278654
33	27.88592797574622	24.941885456073532	26.31503536058818	20.857151207592064
34	20.56018072001443	25.158375943537116	29.351462362234138	24.92998097421432
35	22.05893360079211	23.4985204938863	31.706625093649777	22.735920811671807
36	28.906077915190192	23.317743452000343	27.455655805074446	20.32052282773502
37	22.610888109988856	28.822603973530704	28.813123158636845	19.75338475784359
38	21.41124424646412	30.418945091824902	25.887115572559242	22.282695089151737
39	21.913228445581193	28.817043796374907	27.749846204074185	21.519881553969718
40	24.335042018543902	24.082766801180185	27.108429870127093	24.47376131014882
41	18.82875581056334	23.414476277646735	27.862974423897924	29.893793487892
42	22.76272371693566	26.027545687904407	24.642705154498078	26.567025440661862
43	22.498116311777665	26.17075589182747	26.28694933751915	25.044178458875717
44	21.71505802900274	28.80456903993562	27.992355469254004	21.488017461807644
45	19.13093005368422	32.59325949703208	24.79069140802932	23.485119041254375
46	22.734566409544115	29.64408450613865	26.750297433835673	20.87105165048156
47	22.661998969228698	24.65482348932482	27.9825895170701	24.700588024376387
48	23.25180545367838	22.7908810243272	30.471695490482475	23.485618031511947
49	21.540055017240114	22.625929102038516	31.499686705402567	24.3343291753188
50	19.843416857174017	29.241470652600842	27.850642236103656	23.06447025412148
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	373.0
1	740.5
2	1108.0
3	6123.5
4	11139.0
5	8128.0
6	5117.0
7	5420.0
8	5723.0
9	6072.0
10	6421.0
11	6512.5
12	6604.0
13	6487.5
14	6371.0
15	6119.5
16	5868.0
17	5500.5
18	5133.0
19	5123.5
20	5114.0
21	5322.5
22	5531.0
23	6260.5
24	6990.0
25	7995.5
26	9001.0
27	10633.5
28	12266.0
29	14079.5
30	15893.0
31	19008.0
32	22123.0
33	25875.0
34	29627.0
35	33741.0
36	37855.0
37	44633.0
38	51411.0
39	67801.5
40	84192.0
41	102299.5
42	120407.0
43	127891.5
44	135376.0
45	136587.5
46	137799.0
47	136337.5
48	134876.0
49	129201.0
50	123526.0
51	111044.5
52	98563.0
53	87462.0
54	76361.0
55	68694.0
56	61027.0
57	55826.5
58	50626.0
59	45770.0
60	40914.0
61	36068.5
62	31223.0
63	26327.5
64	21432.0
65	17327.0
66	13222.0
67	10849.0
68	8476.0
69	7233.0
70	5990.0
71	5082.0
72	4174.0
73	3345.0
74	2516.0
75	1918.5
76	1321.0
77	985.0
78	649.0
79	457.0
80	265.0
81	209.0
82	153.0
83	106.5
84	60.0
85	36.5
86	13.0
87	7.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1402833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.758912536597563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.1438785682481	20.581534403001093
2	6.836851016486527	3.795670987847953
3	2.90426247925102	2.418575044345532
4	1.8212650048062238	2.022253438975276
5	1.3679066963324749	1.8985801170859646
6	1.1269476773509335	1.8769705205343798
7	0.9083668324463402	1.765069281711689
8	0.7907539962089917	1.756037681498432
9	0.6877848047825076	1.7182942415962614
>10	9.093817836898422	54.9521830727168
>50	0.2822445033062306	4.949727249503812
>100	0.034370055144464354	1.4714998107664492
>500	7.752643688660201E-4	0.14892183099199915
>1k	7.752643688660201E-4	0.6446823194244201
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3553	0.2532731978788637	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3356	0.23923018634434745	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2078	0.1481288221762676	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.032149229452115825	0.0
2	0.0	0.0	0.0	0.11034813124584324	0.0
3	0.0	0.0	0.0	0.17892364950068895	0.0
4	0.0	0.0	0.0	0.2815017895929166	0.0
5	7.128432251023464E-5	0.0	0.0	0.47724853920602095	0.0
6	7.128432251023464E-5	0.0	0.0	0.7773555369741089	0.0
7	7.128432251023464E-5	0.0	0.0	0.9829395230936255	0.0
8	7.128432251023464E-5	0.0	0.0	1.5485806222123375	0.0
9	7.128432251023464E-5	0.0	0.0	2.0968996309610626	0.0
10	7.128432251023464E-5	0.0	0.0	2.836260624037216	0.0
11	7.128432251023464E-5	0.0	0.0	3.27487306044269	0.0
12	7.128432251023464E-5	0.0	0.0	3.6709287563095536	0.0
13	7.128432251023464E-5	0.0	0.0	3.858762946124022	0.0
14	7.128432251023464E-5	0.0	0.0	3.939100377593056	0.0
15	7.128432251023464E-5	0.0	0.0	4.0329105460165255	0.0
16	7.128432251023464E-5	0.0	0.0	4.206915577264008	0.0
17	7.128432251023464E-5	0.0	0.0	4.407367092162787	0.0
18	7.128432251023464E-5	0.0	0.0	4.719877562047657	0.0
19	7.128432251023464E-5	0.0	0.0	4.884116641111237	0.0
20	7.128432251023464E-5	0.0	0.0	5.078936694531708	0.0
21	7.128432251023464E-5	0.0	0.0	5.2823108666534075	0.0
22	7.128432251023464E-5	0.0	0.0	5.499799334632134	0.0
23	7.128432251023464E-5	0.0	0.0	5.7445896981322795	0.0
24	7.128432251023464E-5	0.0	0.0	5.925865730275806	0.0
25	7.128432251023464E-5	0.0	0.0	6.080267572832974	0.0
26	7.128432251023464E-5	0.0	0.0	6.227398414494099	0.0
27	1.4256864502046928E-4	0.0	0.0	6.3878594244646365	0.0
28	1.4256864502046928E-4	0.0	0.0	6.55302519972085	0.0
29	1.4256864502046928E-4	0.0	0.0	6.731378574641457	0.0
30	1.4256864502046928E-4	0.0	0.0	6.9730324279511535	0.0
31	1.4256864502046928E-4	0.0	0.0	7.1725572466573	0.0
32	1.4256864502046928E-4	0.0	0.0	7.3563282300886845	0.0
33	1.4256864502046928E-4	0.0	0.0	7.544518841515704	0.0
34	1.4256864502046928E-4	0.0	0.0	7.750459249247772	0.0
35	1.4256864502046928E-4	0.0	0.0	7.9920418182349575	0.0
36	1.4256864502046928E-4	0.0	0.0	8.194631862809045	0.0
37	1.4256864502046928E-4	0.0	0.0	8.397150623060622	0.0
38	1.4256864502046928E-4	0.0	0.0	8.604374148597874	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAT	20	7.857076E-4	44.0	16
TAGCCGT	25	4.4433444E-5	44.0	44
TTAACGA	30	2.5281188E-6	44.0	2
TATACGA	35	1.4463876E-7	44.0	2
CCCGATA	20	7.857076E-4	44.0	24
CGGTCTA	235	0.0	43.063828	31
CGTTTTT	5710	0.0	42.57443	1
AGCGATA	180	0.0	40.333332	8
ACGAGAT	345	0.0	40.173916	5
GAGCGAT	840	0.0	40.07143	7
TATAGCG	100	0.0	39.6	1
TGCGCGA	95	0.0	39.36842	2
CGTTAGG	185	0.0	39.243244	2
AGGGGAA	12330	0.0	39.111115	8
CGACGGT	260	0.0	38.923077	28
GAGGGGA	12045	0.0	38.684933	7
TCACGAC	280	0.0	38.5	25
TTAGCGA	150	0.0	38.13333	2
GTTTTTA	3420	0.0	38.017544	2
CGAGGTT	105	0.0	37.71429	6
>>END_MODULE