FastQCFastQC Report
Thu 26 May 2016
SRR1546441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546441_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1157362
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82190.7101494605836377No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75540.6526912063814088No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG207.856502E-444.0000041
GAACGAT207.856502E-444.00000444
CCAACCG207.856502E-444.00000412
TATAGCG302.5277332E-644.01
CGTTTTT102100.043.202741
GCGCGAC4700.040.255329
GTTTTTA57600.039.9895862
TAGCACG501.3478711E-939.61
GCGATCG501.3478711E-939.69
ATTGCGA1000.039.62
TTTAGCG452.3510438E-839.1111151
TCACGAC850.038.82353225
TCGACGA404.1245767E-738.5000042
CCGTTAC404.1245767E-738.50000443
GCGACCT5050.037.46534711
ACGAGAT3000.037.45
CACGACC1300.037.23076627
CTTGCGA651.0913936E-1137.2307662
TTAGCGA950.037.0526282
TTATTTT64800.036.700626
Produced by FastQC (version 0.11.5)