##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546441_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1157362
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.551463587019445	31.0	31.0	34.0	30.0	34.0
2	31.927576678688258	31.0	31.0	34.0	30.0	34.0
3	31.990133596921275	33.0	31.0	34.0	30.0	34.0
4	35.69935940526819	37.0	35.0	37.0	33.0	37.0
5	35.65319407410991	37.0	35.0	37.0	33.0	37.0
6	35.69977414153912	37.0	35.0	37.0	33.0	37.0
7	36.053507891221585	37.0	35.0	37.0	35.0	37.0
8	18.253623326150333	26.0	0.0	37.0	0.0	37.0
9	27.82702041366487	25.0	17.0	39.0	17.0	39.0
10	33.64600963224989	32.0	32.0	39.0	27.0	39.0
11	35.833646689626924	37.0	35.0	39.0	32.0	39.0
12	36.555527138440695	37.0	35.0	39.0	33.0	39.0
13	36.740077866734865	39.0	35.0	39.0	33.0	39.0
14	37.90750430720898	40.0	37.0	41.0	33.0	41.0
15	38.14554305394509	40.0	37.0	41.0	33.0	41.0
16	38.21172718648098	40.0	37.0	41.0	33.0	41.0
17	38.16639737610186	40.0	37.0	41.0	33.0	41.0
18	38.09145798807979	40.0	37.0	41.0	33.0	41.0
19	38.05537938864418	40.0	37.0	41.0	34.0	41.0
20	37.92701937682419	40.0	36.0	41.0	33.0	41.0
21	37.79382336727834	40.0	36.0	41.0	33.0	41.0
22	37.795799412802566	40.0	36.0	41.0	33.0	41.0
23	37.63610780378136	39.0	35.0	41.0	33.0	41.0
24	37.42924858428046	39.0	35.0	41.0	33.0	41.0
25	37.28989978934854	39.0	35.0	41.0	33.0	41.0
26	37.23755056758387	39.0	35.0	41.0	33.0	41.0
27	37.09836853119422	39.0	35.0	41.0	32.0	41.0
28	37.069755184635405	39.0	35.0	41.0	32.0	41.0
29	36.953276502943766	39.0	35.0	41.0	32.0	41.0
30	36.81455499662163	39.0	35.0	40.0	31.0	41.0
31	36.73508461483961	39.0	35.0	41.0	31.0	41.0
32	36.42305778140288	39.0	35.0	41.0	30.0	41.0
33	36.19398425039011	39.0	35.0	41.0	30.0	41.0
34	35.963363234666424	39.0	35.0	41.0	28.0	41.0
35	35.735141641076865	39.0	35.0	41.0	26.0	41.0
36	35.58023677984934	39.0	35.0	41.0	25.0	41.0
37	35.50427091955672	39.0	35.0	41.0	25.0	41.0
38	35.41207850266382	39.0	35.0	41.0	24.0	41.0
39	35.328021828952394	39.0	35.0	41.0	24.0	41.0
40	35.24734871198467	39.0	35.0	40.0	24.0	41.0
41	35.21387344668306	39.0	35.0	40.0	23.0	41.0
42	35.04642886149709	38.0	35.0	40.0	23.0	41.0
43	35.047074294818735	38.0	35.0	40.0	23.0	41.0
44	34.79390285839694	38.0	34.0	40.0	23.0	41.0
45	34.70492119146818	38.0	34.0	40.0	23.0	41.0
46	34.70742688977174	38.0	34.0	40.0	23.0	41.0
47	34.590957712452976	38.0	34.0	40.0	23.0	41.0
48	34.57774663415595	38.0	34.0	40.0	23.0	41.0
49	34.53697114645202	38.0	34.0	40.0	23.0	41.0
50	33.94683858637142	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	3.0
14	14.0
15	22.0
16	73.0
17	144.0
18	397.0
19	859.0
20	1679.0
21	2799.0
22	4539.0
23	6704.0
24	10338.0
25	15795.0
26	21513.0
27	24122.0
28	23790.0
29	24041.0
30	26868.0
31	33135.0
32	41719.0
33	55178.0
34	81290.0
35	121958.0
36	117545.0
37	177795.0
38	195639.0
39	169400.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.0492637567157	22.176812440705675	26.959412871685785	18.81451093089284
2	36.328046021901535	23.40183970097515	27.891359833829	12.37875444329432
3	27.595946644178742	23.760932188891633	35.76409109682191	12.87903007010771
4	24.285832781791694	26.234402028060366	35.45675423938232	14.023010950765622
5	21.993118834038096	30.286634605248835	34.00146194535504	13.718784615358029
6	19.64337864903116	38.8709841864516	31.166393919966268	10.31924324455097
7	82.71007688173623	3.225784153963928	12.127320579040957	1.9368183852588907
8	42.14256213699776	50.719221816510306	5.8623835930331225	1.2758324534588141
9	78.43328189451529	4.867534963131673	13.549433971393565	3.1497491709594754
10	38.49927680362756	32.59058099367355	18.301966022731005	10.608176179967893
11	32.85298808842869	22.020595111987433	30.963432357378245	14.162984442205637
12	30.062763422334587	21.199935715878006	31.740198831480555	16.997102030306856
13	22.529165464219492	27.830531847425437	32.987345359533144	16.652957328821923
14	17.45175666731757	30.909603045546685	34.27933524688041	17.35930504025534
15	16.338103376471665	24.418289178321043	42.41093106564757	16.83267637955972
16	19.426851754248023	22.378823566006144	40.69876149381093	17.49556318593491
17	19.76667628624406	22.334325820270582	33.365532996590524	24.533464896894834
18	21.506149329250484	23.832387792237867	35.2802321140663	19.381230764445352
19	24.396429120707264	25.959725651956777	30.862167584558676	18.781677642777282
20	26.21141872637947	25.066487408434007	30.08298181554259	18.639112049643934
21	22.841945735215084	26.670998356607527	32.37491813278818	18.112137775389204
22	21.751794166388734	23.04706738254755	32.69210497666244	22.509033474401267
23	19.72105529644139	27.158054264784916	31.864274099201463	21.256616339572236
24	20.136914811441883	24.500113188440608	36.60375923868246	18.759212761435055
25	19.835194174337847	25.84627800117854	34.49240600607243	19.82612181841118
26	18.51572800904125	29.53924528367097	31.680666895923657	20.26435981136412
27	19.53476958808048	26.62511815663552	33.680041335381674	20.160070919902328
28	18.47814253448791	26.262569533127923	36.67512843863891	18.584159493745258
29	18.978590968080862	24.524046927409056	36.130355066089955	20.36700703842013
30	21.145587983707777	25.076423798258453	33.752706586184786	20.025281631848983
31	24.724675598473077	24.467884724053494	30.38548008315462	20.421959594318803
32	24.63092792056418	25.730151845317195	30.202218493435932	19.43670174068269
33	23.55494650766139	25.35930849639093	30.618078008436427	20.467666987511254
34	19.624888323618713	27.38831929854272	31.513130723144528	21.473661654694038
35	20.04999300132543	26.200704706047027	32.1994328481495	21.549869444478045
36	24.146809727639234	26.99120931912401	28.786844565485993	20.075136387750764
37	20.930184332991754	28.80887742987933	29.085627487337582	21.175310749791336
38	21.72086175284829	29.42588403628251	27.369742569740495	21.483511641128704
39	21.253073800591345	27.46452708832673	28.215718159054816	23.06668095202711
40	22.859917640288863	25.340040540470483	28.97408071113446	22.825961108106192
41	19.578748913477373	24.771333428953085	29.785754154706996	25.864163502862542
42	22.15028660004389	26.744441237918647	27.425213545977833	23.680058616059625
43	21.583480363101607	26.328149705969267	28.81241996886022	23.27594996206891
44	21.228708044674008	27.948213264302783	29.94231709698435	20.88076159403886
45	20.450731923114805	28.799286653613994	28.14253448791303	22.60744693535817
46	22.191587420357674	28.011201335450792	28.78338842989488	21.013822814296653
47	21.439359508952254	26.268272156853257	29.45595241592518	22.836415918269307
48	22.052132349256325	24.477389096929052	30.661106896545764	22.80937165726886
49	21.533452800420267	24.14041587679568	31.081805001373812	23.24432632141024
50	19.93352123190497	27.089536376691132	30.44959139836974	22.527350993034158
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	457.0
1	833.0
2	1209.0
3	13346.5
4	25484.0
5	17731.5
6	9979.0
7	10045.5
8	10112.0
9	10382.0
10	10652.0
11	10634.0
12	10616.0
13	10168.5
14	9721.0
15	9067.0
16	8413.0
17	8107.0
18	7801.0
19	7553.0
20	7305.0
21	7426.0
22	7547.0
23	7986.0
24	8425.0
25	9847.0
26	11269.0
27	13191.0
28	15113.0
29	17326.5
30	19540.0
31	22449.5
32	25359.0
33	28731.5
34	32104.0
35	35481.0
36	38858.0
37	43354.0
38	47850.0
39	54866.0
40	61882.0
41	70312.0
42	78742.0
43	83685.0
44	88628.0
45	92138.0
46	95648.0
47	96672.5
48	97697.0
49	92362.0
50	87027.0
51	77694.5
52	68362.0
53	61139.0
54	53916.0
55	50480.5
56	47045.0
57	44451.0
58	41857.0
59	39123.0
60	36389.0
61	33351.0
62	30313.0
63	25744.0
64	21175.0
65	17470.5
66	13766.0
67	11802.5
68	9839.0
69	8514.5
70	7190.0
71	5979.0
72	4768.0
73	3701.5
74	2635.0
75	1925.0
76	1215.0
77	1022.5
78	830.0
79	622.0
80	414.0
81	279.5
82	145.0
83	92.0
84	39.0
85	27.0
86	15.0
87	11.5
88	8.0
89	4.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1157362.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.332858208455065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.34075558212334	26.58029351796673
2	8.060235847586847	6.179437557445065
3	4.539910470209635	5.220832330008795
4	3.2452678250962994	4.976015655515112
5	2.5961362088029705	4.9758660590940185
6	2.083939128313421	4.793000587241389
7	1.7412048446676296	4.672175089576349
8	1.5385952600859034	4.7183003156059184
9	1.2301223103544823	4.243869369168797
>10	5.5826815390168765	30.352334072775218
>50	0.025327913538481624	0.6731535830182224
>100	0.014466807418789338	1.028697680601044
>500	9.041751901996293E-4	0.21930874151005122
>1k	0.0	0.0
>5k	4.5208759509981464E-4	1.3667154404733457
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8219	0.7101494605836377	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7554	0.6526912063814088	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02773548811866987	0.0
2	0.0	0.0	0.0	0.0896867185893437	0.0
3	0.0	0.0	0.0	0.16356161684935222	0.0
4	0.0	0.0	0.0	0.26188867441647473	0.0
5	0.0	0.0	0.0	0.4816988980111668	0.0
6	0.0	0.0	0.0	0.8984224469094372	0.0
7	0.0	0.0	0.0	1.1896882738503598	0.0
8	0.0	0.0	0.0	1.7731703650197606	0.0
9	0.0	0.0	0.0	2.458608456126951	0.0
10	0.0	0.0	0.0	3.166597831966144	0.0
11	0.0	0.0	0.0	3.5879007605226367	0.0
12	0.0	0.0	0.0	3.9514862247075677	0.0
13	0.0	0.0	0.0	4.162656109324481	0.0
14	0.0	0.0	0.0	4.275758146543605	0.0
15	0.0	0.0	0.0	4.377282129532506	0.0
16	0.0	0.0	0.0	4.525204732832078	0.0
17	0.0	0.0	0.0	4.68643345815743	0.0
18	0.0	0.0	0.0	4.942965122407682	0.0
19	0.0	0.0	0.0	5.078877654528142	0.0
20	0.0	0.0	0.0	5.237773488329494	0.0
21	0.0	0.0	0.0	5.3875105628144	0.0
22	0.0	0.0	0.0	5.556515593219753	0.0
23	0.0	0.0	0.0	5.7485039253059975	0.0
24	0.0	0.0	0.0	5.90619011165046	0.0
25	0.0	0.0	0.0	6.038128087841142	0.0
26	0.0	0.0	0.0	6.173435796233158	0.0
27	0.0	0.0	0.0	6.311854026657174	0.0
28	8.640338977778776E-5	0.0	0.0	6.45727093165319	0.0
29	8.640338977778776E-5	0.0	0.0	6.611414579016764	0.0
30	8.640338977778776E-5	0.0	0.0	6.810833602623898	0.0
31	8.640338977778776E-5	0.0	0.0	6.984245205907918	0.0
32	8.640338977778776E-5	0.0	0.0	7.172777402403051	0.0
33	8.640338977778776E-5	0.0	0.0	7.343424097214182	0.0
34	8.640338977778776E-5	0.0	0.0	7.519946222530202	0.0
35	8.640338977778776E-5	0.0	0.0	7.712712185124447	0.0
36	8.640338977778776E-5	0.0	0.0	7.890962378236023	0.0
37	8.640338977778776E-5	0.0	0.0	8.082950710322267	0.0
38	8.640338977778776E-5	0.0	0.0	8.29645348646318	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCG	20	7.856502E-4	44.000004	1
GAACGAT	20	7.856502E-4	44.000004	44
CCAACCG	20	7.856502E-4	44.000004	12
TATAGCG	30	2.5277332E-6	44.0	1
CGTTTTT	10210	0.0	43.20274	1
GCGCGAC	470	0.0	40.25532	9
GTTTTTA	5760	0.0	39.989586	2
TAGCACG	50	1.3478711E-9	39.6	1
GCGATCG	50	1.3478711E-9	39.6	9
ATTGCGA	100	0.0	39.6	2
TTTAGCG	45	2.3510438E-8	39.111115	1
TCACGAC	85	0.0	38.823532	25
TCGACGA	40	4.1245767E-7	38.500004	2
CCGTTAC	40	4.1245767E-7	38.500004	43
GCGACCT	505	0.0	37.465347	11
ACGAGAT	300	0.0	37.4	5
CACGACC	130	0.0	37.230766	27
CTTGCGA	65	1.0913936E-11	37.230766	2
TTAGCGA	95	0.0	37.052628	2
TTATTTT	6480	0.0	36.70062	6
>>END_MODULE