FastQCFastQC Report
Thu 26 May 2016
SRR1546440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546440_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1533352
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100800.6573833014206784No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93500.6097751853455697No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA207.8573066E-444.02
CGACGGT1500.044.028
TGCGCGA800.044.02
TATTACG502.7284841E-1144.01
TCGCGGA408.3164196E-944.02
CTTACGA351.446515E-744.02
CGTTTTT128500.043.4007761
AGCGATC1050.041.9047628
GCGAGGT2600.040.6153835
TAGGGCG1800.040.3333325
GTTTTTA73900.039.9810562
GAGCGAT8750.039.977147
GCGCGAC2800.039.2857139
CTATGCG452.3517714E-839.111111
CGAGACT1750.038.971436
CACGACG1700.038.82353226
CGGGAAC2500.038.726
CGTAAGA1200.038.52
TGTACGA1150.038.260872
CTAGCGA1150.038.260872
Produced by FastQC (version 0.11.5)