FastQCFastQC Report
Thu 26 May 2016
SRR1546439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546439_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences934199
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45760.48983139566623385No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44210.4732396416609309No Hit
GAATCTATCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT28940.30978410381514004No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC26290.2814175566447834No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20160.21579984564316598No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19440.20809270829876717No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC17130.1833656426521544No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC15420.1650611914592073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14580.15606953122407538No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT13850.14825535030544884No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG13260.14193977942601094No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGA207.855723E-444.0000041
ACAACAC207.855723E-444.00000415
TTAGGTC207.855723E-444.0000045
TCGTTAG408.309144E-944.0000042
GTACCGA207.855723E-444.0000048
GCAAGTC207.855723E-444.00000415
TCACCGT207.855723E-444.00000416
CATATAG207.855723E-444.0000042
CTCCGAC207.855723E-444.00000428
GCGAATG408.309144E-944.0000041
TCATTAG207.855723E-444.0000042
TGAACGA207.855723E-444.00000427
ATTCGAT800.044.00000414
CGTCTAA207.855723E-444.00000443
ATCGTGC207.855723E-444.00000428
CGTTAAG650.044.0000042
TGCATAC207.855723E-444.00000424
GTTACAT207.855723E-444.00000429
TCGGATC207.855723E-444.00000422
TGACCAT207.855723E-444.00000419
Produced by FastQC (version 0.11.5)