##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546439_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	934199
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.476027056333823	31.0	31.0	33.0	30.0	34.0
2	31.643148836596914	31.0	31.0	34.0	30.0	34.0
3	31.664205378083256	31.0	31.0	34.0	30.0	34.0
4	35.564051128292796	37.0	35.0	37.0	33.0	37.0
5	26.997791691063682	35.0	25.0	37.0	0.0	37.0
6	31.24087052116305	35.0	26.0	37.0	17.0	37.0
7	15.106035223758536	0.0	0.0	32.0	0.0	37.0
8	25.10038332303931	17.0	17.0	35.0	17.0	37.0
9	33.053750860362726	32.0	32.0	37.0	28.0	39.0
10	36.00776494087448	37.0	35.0	39.0	32.0	39.0
11	36.97193852701619	39.0	37.0	39.0	34.0	39.0
12	36.631141758875785	39.0	35.0	39.0	33.0	39.0
13	35.90059505522913	37.0	35.0	39.0	31.0	39.0
14	37.16639388395834	39.0	35.0	41.0	32.0	41.0
15	37.53937865486904	40.0	35.0	41.0	33.0	41.0
16	37.75071050172394	40.0	35.0	41.0	33.0	41.0
17	37.767957362403514	40.0	35.0	41.0	33.0	41.0
18	37.71231611251992	39.0	35.0	41.0	33.0	41.0
19	37.66390565607542	39.0	36.0	41.0	33.0	41.0
20	37.600704988979864	39.0	35.0	41.0	33.0	41.0
21	37.44312293205195	39.0	35.0	41.0	33.0	41.0
22	37.31280273260836	39.0	35.0	41.0	33.0	41.0
23	37.200879041831556	38.0	35.0	41.0	33.0	41.0
24	37.03720727596583	38.0	35.0	41.0	33.0	41.0
25	36.88560253222279	38.0	35.0	40.0	33.0	41.0
26	36.625277911879586	38.0	35.0	40.0	32.0	41.0
27	36.349566848176885	38.0	35.0	40.0	32.0	41.0
28	36.42735220226098	38.0	35.0	40.0	32.0	41.0
29	36.43297091947219	38.0	35.0	40.0	32.0	41.0
30	36.24826402083496	38.0	35.0	40.0	31.0	41.0
31	35.97471095558869	37.0	35.0	40.0	31.0	41.0
32	35.6107702962645	37.0	34.0	40.0	30.0	41.0
33	35.362722503449476	37.0	34.0	40.0	29.0	41.0
34	35.09056100466817	37.0	34.0	40.0	27.0	41.0
35	34.91299284199619	37.0	34.0	40.0	27.0	41.0
36	34.65773673489267	37.0	34.0	40.0	25.0	41.0
37	34.419069170487234	37.0	34.0	40.0	24.0	41.0
38	34.273808899388676	36.0	33.0	40.0	24.0	40.0
39	34.25984399469492	36.0	33.0	40.0	23.0	41.0
40	34.02070757943436	36.0	33.0	40.0	23.0	40.0
41	33.90348630216902	36.0	33.0	40.0	23.0	40.0
42	33.8905500862236	36.0	33.0	40.0	23.0	40.0
43	33.770106797374005	36.0	33.0	40.0	22.0	40.0
44	33.56515581797883	35.0	33.0	40.0	22.0	40.0
45	33.25345991592798	35.0	33.0	39.0	20.0	40.0
46	33.28184144919872	35.0	33.0	39.0	20.0	40.0
47	33.14878842730511	35.0	33.0	39.0	20.0	40.0
48	33.14659724534066	35.0	33.0	39.0	20.0	40.0
49	33.08891360406081	35.0	33.0	39.0	20.0	40.0
50	33.02796941550997	35.0	33.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	7.0
14	7.0
15	17.0
16	68.0
17	183.0
18	468.0
19	979.0
20	1794.0
21	3050.0
22	4766.0
23	7233.0
24	10710.0
25	15234.0
26	19396.0
27	20332.0
28	20210.0
29	22220.0
30	26491.0
31	35494.0
32	49731.0
33	97755.0
34	89597.0
35	99753.0
36	103922.0
37	192420.0
38	93779.0
39	18583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.093375180234617	22.108672777427508	28.367189431802004	18.43076261053587
2	31.620564783306342	25.76892075457156	29.777060347955842	12.833454114166255
3	25.58641146051323	25.223961918178034	36.082140957119414	13.107485664189323
4	23.06425076455873	26.31002602229289	36.43302979343802	14.192693419710361
5	17.39918368570294	46.24485789430303	25.178468399131233	11.177490020862793
6	20.577949665970525	37.78049430581707	30.44608268687935	11.195473341333056
7	36.5601975596206	55.71500290623304	6.422079235794515	1.30272029835185
8	81.41488055542769	5.173844116724595	10.600846286497845	2.8104290413498623
9	78.39935602585744	4.678232368050062	12.129107395747587	4.793304210344905
10	59.175828704590785	16.538446305337516	15.531273315428512	8.754451674643198
11	55.36507746208249	14.919840419439542	19.171611187766207	10.543470930711765
12	46.91441545109768	18.973045357573707	23.443720235196142	10.668818956132473
13	19.203510172886077	47.66382751426623	24.172151757816053	8.960510555031636
14	11.068091488002022	49.627755970622964	28.6860722394265	10.618080301948513
15	8.540257482613448	20.779405672667174	60.08002577609267	10.600311068626706
16	9.832915684987888	14.723415460731601	55.692309668496755	19.751359185783755
17	11.073657753861866	15.425835394814166	31.39577327742804	42.10473357389593
18	18.539947056248188	21.544124966950296	39.42693152101426	20.488996455787255
19	29.078386938971246	22.142284459735023	27.027110926044667	21.752217675249064
20	31.719687133041248	21.697518408818677	25.916105669134737	20.66668878900534
21	20.200942197540353	29.223109851327177	29.612641417942005	20.963306533190465
22	19.883343912806588	24.253504874229154	26.26025076027699	29.602900452687276
23	16.535020910962224	32.23553011724483	26.3432095303035	24.886239441489447
24	18.4987352801705	21.52774730009345	43.57272915085544	16.400788268880614
25	13.470470424395659	24.770632381323466	40.91505128992859	20.843845904352285
26	13.530735956685888	34.80200685292962	30.508703177802587	21.158554012581902
27	15.17096464457787	34.89984467977379	31.46952630007097	18.45966437557737
28	11.130604935351034	28.236596271244135	44.14755314445851	16.485245648946318
29	11.536514168822702	23.85294781946887	42.243140915372415	22.367397096336006
30	15.174390038953156	30.857130011913952	34.61189746510112	19.356582484031776
31	29.705769327520155	26.429379607556847	25.196130588878813	18.668720476044186
32	31.752014292458032	26.60653672290379	26.81452238762833	14.826926597009846
33	28.206517026886136	26.97851314334526	24.977547610305727	19.837422219462876
34	17.434186934475417	26.9867554985608	29.95796398840076	25.621093578563027
35	18.934937845148625	24.74997297149751	31.750622725993072	24.564466457360798
36	32.3218072380724	22.56703336227078	26.1677651121442	18.943394287512618
37	18.44125288081019	32.54745509254452	30.581064633980553	18.430227392664733
38	19.127937409481277	35.42211027843104	22.472942060524577	22.977010251563105
39	19.08148049826643	32.68768217478289	28.020582338452517	20.210254988498168
40	23.674613224805423	24.98215048399752	26.1164912400891	25.226745051107958
41	15.423480436181155	22.816873064518372	27.044666072218014	34.71498042708245
42	21.40678806121608	24.967699601476774	24.330362160524686	29.295150176782464
43	22.15694942940423	25.3961950291105	24.83988957384883	27.606965967636445
44	18.11423476154438	31.555696377324317	28.580527275237934	21.74954158589337
45	15.289461881248002	38.483556501345	22.4622377031018	23.764743914305196
46	22.5038776534764	33.15150198191178	25.71614827247728	18.628472092134547
47	21.363435413653836	25.07431500140762	28.90133686719853	24.66091271774001
48	23.23241621967054	21.768381254957454	31.57667691787296	23.422525607499047
49	19.986748005510606	21.365362197989935	34.795691282050186	23.852198514449277
50	17.97272315641528	33.736602158640714	26.604502894993466	21.686171789950535
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1859.0
1	2116.0
2	2373.0
3	8321.0
4	14269.0
5	10277.5
6	6286.0
7	6780.0
8	7274.0
9	8024.0
10	8774.0
11	8824.0
12	8874.0
13	8440.0
14	8006.0
15	7481.0
16	6956.0
17	6319.0
18	5682.0
19	5363.5
20	5045.0
21	4747.5
22	4450.0
23	4160.0
24	3870.0
25	4616.5
26	5363.0
27	5424.0
28	5485.0
29	6310.0
30	7135.0
31	8529.5
32	9924.0
33	11926.0
34	13928.0
35	15680.0
36	17432.0
37	22764.5
38	28097.0
39	44487.0
40	60877.0
41	81264.0
42	101651.0
43	112510.0
44	123369.0
45	117453.5
46	111538.0
47	98753.5
48	85969.0
49	79347.0
50	72725.0
51	65034.0
52	57343.0
53	48836.5
54	40330.0
55	34049.0
56	27768.0
57	25025.0
58	22282.0
59	20644.5
60	19007.0
61	16626.5
62	14246.0
63	12148.0
64	10050.0
65	8351.0
66	6652.0
67	5282.5
68	3913.0
69	3169.0
70	2425.0
71	1873.5
72	1322.0
73	1062.5
74	803.0
75	600.5
76	398.0
77	311.5
78	225.0
79	182.0
80	139.0
81	95.0
82	51.0
83	35.0
84	19.0
85	12.0
86	5.0
87	4.0
88	3.0
89	3.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	934199.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.199454623921188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.20978115492451	17.584139425194742
2	6.023765908427943	2.67448635898539
3	2.1337023899047933	1.4210108815693097
4	1.1592452351638252	1.0293844798406473
5	0.7515854214079647	0.8342393229273399
6	0.5970358893467426	0.7952322680643267
7	0.48045294566295504	0.7466055356321829
8	0.38263688264820434	0.6795464091029976
9	0.36952935953605837	0.7383015224304648
>10	6.9935598916282675	37.9064050791263
>50	1.6961763964049685	25.88122300959191
>100	0.19210426263050212	6.044616142723688
>500	0.004963934434896696	0.8102758736522574
>1k	0.0054603278783863654	2.854533691158449
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4576	0.48983139566623385	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4421	0.4732396416609309	No Hit
GAATCTATCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT	2894	0.30978410381514004	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	2629	0.2814175566447834	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2016	0.21579984564316598	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1944	0.20809270829876717	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	1713	0.1833656426521544	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	1542	0.1650611914592073	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1458	0.15606953122407538	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT	1385	0.14825535030544884	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG	1326	0.14193977942601094	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03928499174158825	0.0
2	0.0	0.0	0.0	0.17009223944791207	0.0
3	0.0	0.0	0.0	0.21494349704934387	0.0
4	0.0	0.0	0.0	0.3316209929576033	0.0
5	0.0	0.0	0.0	0.624492212044757	0.0
6	0.0	0.0	0.0	0.8516386765560657	0.0
7	0.0	0.0	0.0	1.0412128465134303	0.0
8	0.0	0.0	0.0	1.7077731832296974	0.0
9	0.0	0.0	0.0	1.9247505081893685	0.0
10	0.0	0.0	0.0	2.900452687275409	0.0
11	0.0	0.0	0.0	3.267183972579718	0.0
12	0.0	0.0	0.0	3.9076256771844116	0.0
13	0.0	0.0	0.0	4.075684088721996	0.0
14	0.0	0.0	0.0	4.136913013180275	0.0
15	0.0	0.0	0.0	4.2579792956318725	0.0
16	0.0	0.0	0.0	4.433744844513856	0.0
17	0.0	0.0	0.0	4.624068319490815	0.0
18	0.0	0.0	0.0	4.925610068090418	0.0
19	0.0	0.0	0.0	5.059414535875119	0.0
20	0.0	0.0	0.0	5.166672197251335	0.0
21	0.0	0.0	0.0	5.310431717439218	0.0
22	0.0	0.0	0.0	5.444236185223919	0.0
23	0.0	0.0	0.0	5.611759378890365	0.0
24	0.0	0.0	0.0	5.729079136243991	0.0
25	0.0	0.0	0.0	5.8164266928138435	0.0
26	0.0	0.0	0.0	5.914371563232245	0.0
27	0.0	0.0	0.0	6.015420697303251	0.0
28	0.0	0.0	0.0	6.100948513111232	0.0
29	0.0	0.0	0.0	6.193113030521334	0.0
30	0.0	0.0	0.0	6.324883670395708	0.0
31	0.0	0.0	0.0	6.454406395211299	0.0
32	0.0	0.0	0.0	6.558880923657593	0.0
33	0.0	0.0	0.0	6.6639977135492545	0.0
34	0.0	0.0	0.0	6.776607553636859	0.0
35	0.0	0.0	0.0	6.942203962967205	0.0
36	0.0	0.0	0.0	7.066802683368318	0.0
37	0.0	0.0	0.0	7.17716460839714	0.0
38	0.0	0.0	0.0	7.282923659734168	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCGA	20	7.855723E-4	44.000004	1
ACAACAC	20	7.855723E-4	44.000004	15
TTAGGTC	20	7.855723E-4	44.000004	5
TCGTTAG	40	8.309144E-9	44.000004	2
GTACCGA	20	7.855723E-4	44.000004	8
GCAAGTC	20	7.855723E-4	44.000004	15
TCACCGT	20	7.855723E-4	44.000004	16
CATATAG	20	7.855723E-4	44.000004	2
CTCCGAC	20	7.855723E-4	44.000004	28
GCGAATG	40	8.309144E-9	44.000004	1
TCATTAG	20	7.855723E-4	44.000004	2
TGAACGA	20	7.855723E-4	44.000004	27
ATTCGAT	80	0.0	44.000004	14
CGTCTAA	20	7.855723E-4	44.000004	43
ATCGTGC	20	7.855723E-4	44.000004	28
CGTTAAG	65	0.0	44.000004	2
TGCATAC	20	7.855723E-4	44.000004	24
GTTACAT	20	7.855723E-4	44.000004	29
TCGGATC	20	7.855723E-4	44.000004	22
TGACCAT	20	7.855723E-4	44.000004	19
>>END_MODULE