FastQCFastQC Report
Thu 26 May 2016
SRR1546438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546438_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences365580
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17480.4781443186169922No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG15730.4302751791673505No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.32933967941353465No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.3260572241369878No Hit
GAATCTATCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC10380.2839323814213031No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG10100.2762733191093605No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGC8150.22293342086547405No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC7760.21226544121669674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6210.16986706056129985No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGC4440.1214508452322337No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3770.10312380327151376No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAT302.161425E-645.00000414
CCTGTCA302.161425E-645.0000041
GACCCAC302.161425E-645.00000412
CTACGGG302.161425E-645.0000043
TCATGCA302.161425E-645.00000424
AGTCTAG302.161425E-645.0000042
CACAGCG302.161425E-645.00000445
GGACGAT302.161425E-645.0000048
TCGTAAG302.161425E-645.0000042
GTTATAA600.045.0000041
CGAAGGA302.161425E-645.0000044
TCACGAC502.1827873E-1145.025
GTTGATA253.8855687E-545.01
CTTGTGC207.0269685E-445.027
CTTAACA207.0269685E-445.01
ATGGGTA351.2088822E-745.05
AAGTATA207.0269685E-445.01
TTAGGCT351.2088822E-745.05
AGGTAAC207.0269685E-445.07
GGTACTT253.8855687E-545.09