##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546438_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	365580
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.940551999562338	31.0	31.0	33.0	30.0	33.0
2	31.091621532906615	31.0	31.0	33.0	30.0	34.0
3	31.00700804201543	31.0	31.0	34.0	28.0	34.0
4	34.96463427977461	35.0	35.0	37.0	32.0	37.0
5	32.69764210295968	35.0	35.0	37.0	28.0	37.0
6	33.890223754034686	35.0	35.0	37.0	28.0	37.0
7	15.808474205372285	0.0	0.0	35.0	0.0	37.0
8	25.109308496088406	17.0	17.0	35.0	17.0	37.0
9	32.62038951802615	32.0	32.0	37.0	27.0	39.0
10	35.441364407243285	37.0	34.0	37.0	32.0	39.0
11	36.38176596093878	37.0	35.0	39.0	32.0	39.0
12	35.92220854532523	37.0	35.0	39.0	32.0	39.0
13	35.231284534164885	37.0	35.0	39.0	30.0	39.0
14	36.229356091689915	38.0	35.0	40.0	30.0	41.0
15	36.75562120466109	38.0	35.0	40.0	31.0	41.0
16	37.02871601291099	38.0	35.0	40.0	32.0	41.0
17	36.885729525685214	38.0	35.0	40.0	32.0	41.0
18	36.815747579189235	38.0	35.0	40.0	31.0	41.0
19	36.72092018162919	38.0	35.0	40.0	31.0	41.0
20	36.68454784178566	38.0	35.0	40.0	31.0	41.0
21	36.534843262760546	38.0	35.0	40.0	31.0	41.0
22	36.520734175830185	38.0	35.0	40.0	31.0	41.0
23	36.49577110345205	38.0	35.0	40.0	31.0	41.0
24	36.32934515017233	38.0	34.0	40.0	31.0	41.0
25	36.18437551288363	37.0	34.0	40.0	31.0	41.0
26	35.9939165162208	37.0	34.0	40.0	30.0	41.0
27	35.75666338421139	37.0	34.0	40.0	30.0	41.0
28	35.744488210514795	37.0	34.0	40.0	30.0	41.0
29	35.82168335248099	37.0	34.0	40.0	30.0	41.0
30	35.596837901416926	37.0	34.0	40.0	29.0	41.0
31	35.433133650637345	37.0	34.0	40.0	29.0	41.0
32	35.14889764210296	37.0	34.0	40.0	27.0	41.0
33	34.84810438207779	37.0	34.0	40.0	25.0	41.0
34	34.614716341156516	37.0	34.0	40.0	24.0	41.0
35	34.38080857814979	37.0	33.0	40.0	23.0	41.0
36	34.10533946058318	36.0	33.0	40.0	22.0	41.0
37	33.916146944581214	36.0	33.0	40.0	22.0	41.0
38	33.87505607527764	36.0	33.0	40.0	22.0	41.0
39	33.762145084523226	36.0	33.0	40.0	21.0	41.0
40	33.55205426992724	36.0	33.0	40.0	20.0	41.0
41	33.52402757262432	36.0	33.0	40.0	20.0	41.0
42	33.440852891296025	36.0	33.0	40.0	18.0	41.0
43	33.33815854258986	35.0	32.0	40.0	18.0	41.0
44	33.14128508124077	35.0	32.0	40.0	18.0	41.0
45	33.03312544449915	35.0	32.0	40.0	18.0	41.0
46	33.05306909568357	35.0	32.0	40.0	18.0	41.0
47	33.00895836752557	35.0	32.0	40.0	18.0	41.0
48	33.02810602330543	35.0	32.0	40.0	18.0	41.0
49	32.942773127632805	35.0	32.0	40.0	18.0	41.0
50	32.73391596914492	35.0	32.0	39.0	18.0	40.0
51	32.51778270146069	35.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	23.0
16	47.0
17	149.0
18	296.0
19	627.0
20	1136.0
21	1838.0
22	2919.0
23	3933.0
24	5818.0
25	7337.0
26	8713.0
27	9105.0
28	9203.0
29	10293.0
30	12179.0
31	15535.0
32	20349.0
33	31752.0
34	39289.0
35	36882.0
36	36009.0
37	53145.0
38	50814.0
39	8182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.74544559330379	22.424640297609276	28.3437277750424	17.486186334044532
2	30.495650746758574	26.751189890037747	29.942557032660428	12.810602330543247
3	25.51972208545325	26.371519229717162	34.98276710979813	13.125991575031456
4	23.13228294764484	26.623721210131844	36.08786038623557	14.156135455987744
5	22.58000984736583	34.01498987909623	29.86541933366158	13.53958093987636
6	20.351222714590513	39.388369166803436	29.370862738661852	10.889545379944199
7	35.311286175392524	55.66551780731987	8.08961102904973	0.9335849882378686
8	81.23228841840363	6.793314732753434	9.40724328464358	2.5671535641993546
9	78.10328792603534	4.735215274358554	10.821707970895563	6.339788828710542
10	60.42425734449368	16.733683461896163	14.401498987909623	8.440560205700532
11	56.87619672848624	14.899338038185897	18.015208709448	10.209256523879862
12	46.96810547622955	19.138629027846164	22.139066688549704	11.754198807374582
13	20.280649926144754	48.118606050659224	22.90169046446742	8.699053558728595
14	10.898845669894415	51.17293068548607	27.453361781279064	10.474861863340445
15	8.415941791126428	20.64062585480606	59.239838065539686	11.70359428852782
16	9.74451556430877	14.58805186279337	54.76475737184747	20.902675201050386
17	10.989660265878877	16.572296077465946	30.51233656108102	41.925707095574154
18	18.3303244159965	21.331856228458886	38.93429618688112	21.403523168663494
19	29.541550413042287	21.81246238853329	26.107828655834563	22.538158542589855
20	33.212976639859946	21.44099786640407	25.138410197494395	20.20761529624159
21	20.200503309809072	30.809398763608513	28.35029268559549	20.639805240986924
22	19.62826193993107	24.211937195689043	26.08047486186334	30.07932600251655
23	17.30264237649762	32.611193172493024	25.483068001531812	24.603096449477544
24	18.053777558947427	22.388533289567263	43.430986377810605	16.126702773674708
25	13.124897423272607	25.96914492040046	40.64281415832376	20.26314349800317
26	13.260298703430164	35.263143498003174	30.82717872968981	20.649379068876854
27	15.752229334208657	35.91607856009629	30.349581486952243	17.98211061874282
28	10.860003282455278	29.423108485146887	43.64489304666557	16.07199518573226
29	11.509929427211555	23.789321078833634	42.69653700968325	22.00421248427157
30	15.134307128398708	32.6754745883254	33.510859456206575	18.679358827069315
31	30.230318945237705	26.111931724930248	24.215493188905302	19.442256140926748
32	33.59127961048197	26.132720608348375	25.98008643798895	14.2959133431807
33	29.889490672356256	26.489687619672846	23.919798676076372	19.701023031894525
34	17.594507358170578	26.610864926965373	29.00678374090486	26.787843973959184
35	19.514470157010777	24.375786421576674	31.616335685759616	24.493407735652937
36	32.65687400842497	23.323485967503693	25.33125444499152	18.68838557907982
37	18.08714918759232	33.52207451173478	29.971552054269928	18.419224246402976
38	18.823786859237376	36.24897423272608	21.706056129985228	23.221182778051315
39	18.914054379342417	33.54368400897204	27.375129930521364	20.16713168116418
40	24.115378302970623	24.98304064773784	25.207068220362167	25.694512828929373
41	15.180261502270366	22.675748126265113	26.332129766398598	35.811860605065924
42	21.127249849554133	24.647136057771213	24.064226708244433	30.16138738443022
43	21.710159199080913	25.020515345478415	24.492587121833797	28.776738333606872
44	17.871874829038788	31.175666064883202	29.05547349417364	21.89698561190437
45	15.183817495486624	39.08118606050659	22.350237978007552	23.384758465999234
46	22.48728048580338	34.090212812517095	24.8457246020023	18.576782099677224
47	20.936867443514416	25.736364133705347	28.244980578806278	25.081787843973956
48	22.856009628535478	22.188850593577328	31.88358225285847	23.071557525028723
49	20.328519065594396	21.79823841566825	34.77405766179769	23.099184856939658
50	17.928497182559223	34.83505662235352	25.785327424913834	21.451118770173423
51	17.336561081021937	34.507084632638545	23.14459215493189	25.01176213140763
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	766.0
1	788.5
2	811.0
3	2756.5
4	4702.0
5	3500.5
6	2299.0
7	2431.5
8	2564.0
9	2722.0
10	2880.0
11	2820.5
12	2761.0
13	2687.5
14	2614.0
15	2495.0
16	2376.0
17	2244.5
18	2113.0
19	1927.5
20	1742.0
21	1707.5
22	1673.0
23	1632.0
24	1591.0
25	1641.5
26	2034.0
27	2376.0
28	2517.5
29	2659.0
30	3111.0
31	3563.0
32	4289.0
33	5015.0
34	5716.5
35	6418.0
36	8114.5
37	9811.0
38	14466.0
39	19121.0
40	26961.0
41	34801.0
42	39451.0
43	44101.0
44	42549.5
45	40998.0
46	40746.0
47	40494.0
48	34819.5
49	29145.0
50	26650.5
51	24156.0
52	21761.5
53	19367.0
54	16923.0
55	14479.0
56	12628.5
57	10778.0
58	9600.5
59	8423.0
60	7614.0
61	6805.0
62	5597.5
63	4390.0
64	3758.0
65	3126.0
66	2525.0
67	1924.0
68	1566.5
69	1209.0
70	995.5
71	782.0
72	614.5
73	447.0
74	351.5
75	213.0
76	170.0
77	139.0
78	108.0
79	75.0
80	42.0
81	32.0
82	22.0
83	14.5
84	7.0
85	4.0
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	365580.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.81425970482101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.703216271948	20.778146581733722
2	6.009486064477411	3.3429881217975495
3	2.772984257054238	2.3138551284926017
4	1.7156371872218836	1.9087671313855237
5	1.2767141734042289	1.7755429793945554
6	1.0547630747253276	1.7602472452479474
7	0.9397888583832429	1.829767196233814
8	0.8578072747923364	1.9087419454207044
9	0.7608290629489882	1.9045707433054897
>10	9.667827908856736	54.53929781560999
>50	0.1989553002960071	3.502975274225103
>100	0.03299258748627253	1.6606944778655486
>500	0.002999326135115685	0.6151132258939372
>1k	0.00599865227023137	2.1592921333935005
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1748	0.4781443186169922	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG	1573	0.4302751791673505	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1204	0.32933967941353465	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1192	0.3260572241369878	No Hit
GAATCTATCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	1038	0.2839323814213031	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG	1010	0.2762733191093605	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGC	815	0.22293342086547405	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	776	0.21226544121669674	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	621	0.16986706056129985	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGC	444	0.1214508452322337	No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	377	0.10312380327151376	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04267191859510914	0.0
2	0.0	0.0	0.0	0.19585316483396248	0.0
3	0.0	0.0	0.0	0.23743093167022267	0.0
4	0.0	0.0	0.0	0.38678264675310464	0.0
5	0.0	0.0	0.0	0.7486733409923957	0.0
6	0.0	0.0	0.0	0.9040428907489468	0.0
7	0.0	0.0	0.0	1.0542152196509655	0.0
8	0.0	0.0	0.0	1.6387657968160183	0.0
9	0.0	0.0	0.0	1.8168389955686854	0.0
10	0.0	0.0	0.0	2.8576508561737515	0.0
11	0.0	0.0	0.0	3.130641720006565	0.0
12	0.0	0.0	0.0	3.762514360741835	0.0
13	0.0	0.0	0.0	3.9137808414027027	0.0
14	0.0	0.0	0.0	3.9723179605011216	0.0
15	0.0	0.0	0.0	4.091854040155369	0.0
16	0.0	0.0	0.0	4.2466765140324965	0.0
17	0.0	0.0	0.0	4.413534657256961	0.0
18	0.0	0.0	0.0	4.6651895617922206	0.0
19	0.0	0.0	0.0	4.835603698232945	0.0
20	0.0	0.0	0.0	4.937086273866186	0.0
21	0.0	0.0	0.0	5.060725422616117	0.0
22	0.0	0.0	0.0	5.175064281415833	0.0
23	0.0	0.0	0.0	5.315662782427923	0.0
24	0.0	0.0	0.0	5.431642868865912	0.0
25	0.0	0.0	0.0	5.511242409322173	0.0
26	0.0	0.0	0.0	5.592483177416708	0.0
27	0.0	0.0	0.0	5.6898626839542645	0.0
28	0.0	0.0	0.0	5.765906231194267	0.0
29	0.0	0.0	0.0	5.854258985721319	0.0
30	0.0	0.0	0.0	5.973795065375567	0.0
31	0.0	0.0	0.0	6.078286558345643	0.0
32	0.0	0.0	0.0	6.181410361617156	0.0
33	0.0	0.0	0.0	6.278516330215001	0.0
34	0.0	0.0	0.0	6.383007823185076	0.0
35	0.0	0.0	0.0	6.536736145303354	0.0
36	0.0	0.0	0.0	6.648066086766234	0.0
37	0.0	0.0	0.0	6.751736965917173	0.0
38	0.0	0.0	0.0	6.834618961649981	0.0
39	0.0	0.0	0.0	6.913124350347394	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAT	30	2.161425E-6	45.000004	14
CCTGTCA	30	2.161425E-6	45.000004	1
GACCCAC	30	2.161425E-6	45.000004	12
CTACGGG	30	2.161425E-6	45.000004	3
TCATGCA	30	2.161425E-6	45.000004	24
AGTCTAG	30	2.161425E-6	45.000004	2
CACAGCG	30	2.161425E-6	45.000004	45
GGACGAT	30	2.161425E-6	45.000004	8
TCGTAAG	30	2.161425E-6	45.000004	2
GTTATAA	60	0.0	45.000004	1
CGAAGGA	30	2.161425E-6	45.000004	4
TCACGAC	50	2.1827873E-11	45.0	25
GTTGATA	25	3.8855687E-5	45.0	1
CTTGTGC	20	7.0269685E-4	45.0	27
CTTAACA	20	7.0269685E-4	45.0	1
ATGGGTA	35	1.2088822E-7	45.0	5
AAGTATA	20	7.0269685E-4	45.0	1
TTAGGCT	35	1.2088822E-7	45.0	5
AGGTAAC	20	7.0269685E-4	45.0	7
GGTACTT	25	3.8855687E-5	45.0	9
>>END_MODULE